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一条新的可诱导PC12细胞转化为交感神经原表型的小分子肽链
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作者 李贤慧 胡孝国 +1 位作者 王斌 朱辉 《中国生化药物杂志》 CAS CSCD 2002年第5期217-220,共4页
目的根据神经生长因子 (NGF)的氨基酸序列及其晶体构象资料 ,对 β NGF进行限制性酶解 ,从而获得关键的功能区域片段。方法选择溴化氰在 9位Met处 ,Trypsin在Arg或Lys处裂解 β NGF ,用SephadexG 5 0色谱、DE5 2离子交换色谱和反相高效... 目的根据神经生长因子 (NGF)的氨基酸序列及其晶体构象资料 ,对 β NGF进行限制性酶解 ,从而获得关键的功能区域片段。方法选择溴化氰在 9位Met处 ,Trypsin在Arg或Lys处裂解 β NGF ,用SephadexG 5 0色谱、DE5 2离子交换色谱和反相高效液相色谱进行分离纯化。所得片段用PC12细胞测定活性 ,对活性片段进行氨基酸组成及序列分析。结果从 β NGF裂解片段中获得一可诱导PC12细胞分化的活性片段 ,氨基酸组成及序列分析表明 ,此片段由 16肽GEFSVCDSVSVWVGDK与 14肽HWNSYCTTTHTFVK通过一对二硫键连接而成 ,与 β NGF肽链的10~ 2 5和 75~ 88氨基酸残基序列相对应。生物活性分析表明其最佳作用浓度为 0 .0 0 1~ 0 .1ng/ml。结论此实验获得了 β NGF关键的功能区域片段 ,虽然其空间结构和功能的关系尚需研究探讨 。 展开更多
关键词 交感神经表型 寡聚肽 PC12细胞 生物活性 神经生长因子 小分子神经营养物质
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基于光镜表型的促神经元亚型分化药物初筛法 被引量:2
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作者 高益宁 王丹音 +4 位作者 潘宗富 梅宇钦 王志强 朱丹雁 楼宜嘉 《浙江大学学报(医学版)》 CAS CSCD 北大核心 2012年第4期373-380,共8页
目的:建立基于光镜表型的促胚胎干(embryonic stem,ES)细胞神经元亚型分化药物筛选法,供早期发现促神经再生药初筛用。方法:采用悬滴培养4-/4+法,形成拟胚体,继而贴壁培养向神经细胞分化,光镜确认表型呈神经元样的基础上进一步评价各类... 目的:建立基于光镜表型的促胚胎干(embryonic stem,ES)细胞神经元亚型分化药物筛选法,供早期发现促神经再生药初筛用。方法:采用悬滴培养4-/4+法,形成拟胚体,继而贴壁培养向神经细胞分化,光镜确认表型呈神经元样的基础上进一步评价各类亚型。以10-7mol/L异补骨脂甲素(Isobavachin,IBA)和10-6mol/L淫羊藿苷(Icariin,ICA)为例,分化终点以光镜下轴突为胞体三倍长以上者为神经元样细胞,继续以免疫荧光成像法确认神经元(β-tubulinⅢ阳染),并进一步以特异性抗体(ChAT、GABA)阳染共定位鉴别相应神经元亚型。结果:基于光镜表型评价,在分化终点即可剔除轴突分化不全的细胞,取代了以往免疫荧光成像所需的人力、物力与时间;并避免进一步ELISA或流式细胞术评价的假阳性。光镜显示IBA处理者大部分显示神经元样表型,而ICA处理的细胞却不然,免疫荧光成像结果与此相一致。因此,仅对IBA处理的神经元做亚型鉴定,显示胆碱能神经元以及γ-氨基丁酸能神经元亚型。结论:基于光镜表型的促神经元分化初筛法在化合物促神经元亚型分化上具有简便易行、节约物力与时间,避免假阳性的独到之处;经该法初筛后供后续研究,可见IBA具有促胆碱能和γ-氨基丁酸能神经元分化活性。 展开更多
关键词 胚胎干细胞 神经元/细胞学 神经表型 异补骨脂甲素 淫羊藿苷 乙酰胆碱能神经 Γ-氨基丁酸能神经
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七氟醚麻醉对发育期脑组织的神经毒性作用研究进展 被引量:4
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作者 李帅蓉 陶利军 +1 位作者 江波 王奋珍 《山东医药》 CAS 北大核心 2017年第43期107-110,共4页
七氟醚因麻醉诱导、苏醒快,气道刺激性小,麻醉平稳等优点被广泛应用于产科和儿科麻醉中。发育期脑组织因解剖结构和生理功能不成熟,敏感且易受损。动物研究和临床研究均提示七氟醚麻醉对发育期脑组织有一定的神经毒性,其作用可能与诱导... 七氟醚因麻醉诱导、苏醒快,气道刺激性小,麻醉平稳等优点被广泛应用于产科和儿科麻醉中。发育期脑组织因解剖结构和生理功能不成熟,敏感且易受损。动物研究和临床研究均提示七氟醚麻醉对发育期脑组织有一定的神经毒性,其作用可能与诱导神经元异常凋亡、降低突触可塑性、引发兴奋性/抑制性神经递质失衡、造成神经元表型缺失有关。 展开更多
关键词 七氟醚 七氟醚神经毒性 神经元凋亡 突触可塑性 兴奋性经递质 抑制性神经递质失衡 神经表型缺失 脑组织 发育期脑组织 麻醉
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C型尼曼-匹克病与细胞胆固醇转运
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作者 宋天保 《解剖科学进展》 CAS 2001年第2期167-171,188,共6页
C型尼曼 -匹克病 (NPC)是一种致命的常染色体隐性遗传性疾病 ,游离胆固醇等脂类沉积于溶酶体内 ,导致神经系统退行性变。本文综述了NPC的细胞生化表型 ,神经表型 ,NPC1基因结构及其突变 ,以及NPC1蛋白的分子结构。
关键词 C型尼曼-匹克病 细胞胆固醇 NPC 常染色本隐性遗传性疾病 神经表型
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CgA、CD56、Syn表达对小细胞肺癌预后的影响 被引量:5
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作者 沈剑 钱璐 《中外医疗》 2016年第29期31-33,共3页
目的探讨40例小细胞肺癌中传统神经内分泌(NE)标记物Cg A、CD56、Syn表达对小细胞肺癌(SCLC)的预后影响。方法方便收集该院2013年1月—2015年1月收治的小细胞肺癌住院患者40例。Cg A、CD56、Syn的表达情况依据免疫组化报告,随访所有病... 目的探讨40例小细胞肺癌中传统神经内分泌(NE)标记物Cg A、CD56、Syn表达对小细胞肺癌(SCLC)的预后影响。方法方便收集该院2013年1月—2015年1月收治的小细胞肺癌住院患者40例。Cg A、CD56、Syn的表达情况依据免疫组化报告,随访所有病例至少12个月,最后一次随访日期截止到2016年4月。采用SPSS 20.0统计学软件作统计分析。结果小细胞肺癌中,Cg A阳性率30.0%,CD56阳性率87.5%,Syn阳性率62.5%。Cg A、CD56、Syn的表达均与临床病理参数无关(P>0.05),单因素生存分析(Log.rank检验)显示,NE表型与SCLC的无进展生存相关(P<0.05)。结论 SCLC的预后的影响因素之一是神经内分泌表型,Cg A、CD56、Syn的共同表达,是其无进展生存期较长的影响因素。 展开更多
关键词 神经内分泌表型 CgA CD56 SYN 小细胞肺癌
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论肿瘤转分化
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作者 张众 谢丰培 李连宏 《怀化医专学报》 2004年第2期1-4,共4页
转分化是指原本委任为特殊专能性细胞系的细胞改变为另一类型细胞。在胚胎正常发育过程中的某些阶段,不同类型细胞的转变,是个体发生的重要步骤,化生实质上就是病理条件下的一种转分化现象。转分化可使肿瘤细胞形态表型不同于所来源... 转分化是指原本委任为特殊专能性细胞系的细胞改变为另一类型细胞。在胚胎正常发育过程中的某些阶段,不同类型细胞的转变,是个体发生的重要步骤,化生实质上就是病理条件下的一种转分化现象。转分化可使肿瘤细胞形态表型不同于所来源的正常细胞。肿瘤性转分化见于肿瘤性上皮和神经内分泌细胞表型转变、肿瘤性上皮和间叶表型转变及非神经外胚叶和神经外胚叶表型转变。肿瘤细胞的演进,从最初的单克隆细胞群变为异质性细胞亚群,从原位发展至侵袭性生长以至转移,也是肿瘤细胞分化降低与一系列表型转分化的结果。鉴于这些事实,我们提出肿瘤的分化障碍不只是分化降低未分化与去分化,还应该包括转分化。 展开更多
关键词 肿瘤转分化 上皮表型 间叶表型 神经外胚叶表型 癌侵袭转移
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FANN-Based Surface Water Quality Evaluation Model and Its Application in the Shaoguan Area 被引量:15
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作者 YANG Meini LI Dingfang YANG Jinbo XlONG Wei 《Geo-Spatial Information Science》 2007年第4期303-310,共8页
A fuzzy neural network model is proposed to evaluate water quality. The model contains two parts: first, fuzzy mathematics theory is used to standardize the samples; second, the RBF neural network and the BP neural n... A fuzzy neural network model is proposed to evaluate water quality. The model contains two parts: first, fuzzy mathematics theory is used to standardize the samples; second, the RBF neural network and the BP neural network are used to train the standardized samples. The proposed model was applied to assess the water quality of 16 sections in 9 rivers in the Shaoguan area in 2005. The evaluation result was compared with that of the RBF neural network method and the reported results in the Shaoguan area in 2005. It indicated that the performance of the proposed fuzzy neural network model is practically feasible in the application of water quality assessment and its operation is simple. 展开更多
关键词 fuzzy neural network RBF neural networks BP neural networks water quality evaluation
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Non-linear Chemical Process Modelling and Application in Epichlorhydrine Production Plant Using Wavelet Networks 被引量:3
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作者 黄德先 金以慧 +1 位作者 张杰 A.J.Morris 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2002年第4期435-443,共9页
A type of wavelet neural network, in which the scale function isadopted only, is proposed in this paper for non-linear dynamicprocess modelling. Its network size is decreased significantly andthe weight coefficients c... A type of wavelet neural network, in which the scale function isadopted only, is proposed in this paper for non-linear dynamicprocess modelling. Its network size is decreased significantly andthe weight coefficients can be estimated by a linear algorithm. Thewavelet neural network holds some advantages superior to other typesof neural networks. First, its network structure is easy to specifybased on its theoretical analysis and intuition. Secondly, networktraining does not rely on stochastic gradient type techniques andavoids the problem of poor convergence or undesirable local minima. 展开更多
关键词 WAVELET neural network non-linear system identification hybrid neuralnetwork
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Study on resource quantity of surface water based on phase space reconstruction and neural network 被引量:5
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作者 曹连海 郝仕龙 陈南祥 《Journal of Coal Science & Engineering(China)》 2006年第1期39-42,共4页
Proposed a new method to disclose the complicated non-linearity structure of the water-resource system, introducing chaos theory into the hydrology and water resources field, and combined with the chaos theory and art... Proposed a new method to disclose the complicated non-linearity structure of the water-resource system, introducing chaos theory into the hydrology and water resources field, and combined with the chaos theory and artificial neural networks. Training data construction and networks structure were determined by the phase space reconstruction, and establishing nonlinear relationship of phase points with neural networks, the forecasting model of the resource quantity of the surface water was brought forward. The keystone of the way and the detailed arithmetic of the network training were given. The example shows that the model has highly forecasting precision. 展开更多
关键词 phase space reconstruction neural network resource quantity of the surface water forecasting model
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Mountain ground movement prediction caused by mining based on BP-neural network 被引量:3
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作者 ZHANG He-sheng LIU Li-juan LIU Hong-fu 《Journal of Coal Science & Engineering(China)》 2011年第1期12-15,共4页
Six main influencing factors: slope, aspect, distance, angle, angle of coal seam, and the ratio of depth and thickness, were selected by Grey correlation theory and Grey relational analysis procedure programmed by th... Six main influencing factors: slope, aspect, distance, angle, angle of coal seam, and the ratio of depth and thickness, were selected by Grey correlation theory and Grey relational analysis procedure programmed by the MATLAB software package to select the surface movement and deformation parameters. On this basis, the paper built a BP neural network model that takes the six main influencing factors as input data and corresponding value of ground subsidence as output data. Ground subsidence of the 3406 mining face in Haoyu Coal was predicted by the trained BP neural network. By comparing the prediction and the practices, the research shows that it is feasible to use the 13P neural network to predict mountain mining subsidence. 展开更多
关键词 BP neural network mountain regions mining subsidence Grey theory
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Plasticity as panacea? Nerves, hormones, and the currencies of trade-offs 被引量:1
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作者 Elizabeth BASTIAANS Elizabeth SWANGER 《Current Zoology》 SCIE CAS CSCD 2015年第2期251-264,共14页
Phenotypic plasticity is nearly universal among organisms, and evidence indicates that plasticity can exhibit additive genetic variation and respond to selection. These findings have important implications for our und... Phenotypic plasticity is nearly universal among organisms, and evidence indicates that plasticity can exhibit additive genetic variation and respond to selection. These findings have important implications for our understanding of how plasticity may be constrained and how its mechanistic structure may affect its evolution. Many life history trade-offs may be conceptua- lized as plastic traits, with individuals varying in their position along trade-off axes due to genetic differences, developmental plasticity, or short-term plasticity occurring throughout an individual's lifetime. Behavioral plasticity is key to understanding when organisms are likely to encounter trade-offs, whether those trade-offs can be mitigated, and how the tradc-offs affect the ecology and evolution of populations. In this review, we discuss hormonal and neural mechanisms that may influence how plastic behavioral traits are expressed and evolve. We also outline a classification of life history trade-offs and their mechanistic bases and discuss the currencies most likely to mediate each category of trade-off and bow they are tied to the mechanisms by which animals express their behaviors. 展开更多
关键词 Phenotypic plasticity Life history trade-offs HORMONES Neural tissue
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Single-cell atlas of domestic pig cerebral cortex and hypothalamus 被引量:1
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作者 Jiacheng Zhu Fang Chen +30 位作者 Lihua Luo Weiying Wu Jinxia Dai Jixing Zhong Xiumei Lin Chaochao Chai Peiwen Ding Langchao Liang Shiyou Wang Xiangning Ding Yin Chen Haoyu Wang Jiaying Qiu Feiyue Wang Chengcheng Sun Yuying Zeng Jian Fang Xiaosen Jiang Ping Liu Gen Tang Xin Qiu Xingliang Zhang Yetian Ruan Sanjie Jiang Jiankang Li Shida Zhu Xun Xu Fang Li Zhongmin Liu Gang Cao Dongsheng Chen 《Science Bulletin》 SCIE EI CSCD 2021年第14期1448-1461,M0004,共15页
The brain of the domestic pig(Sus scrofa domesticus)has drawn considerable attention due to its high similarities to that of humans.However,the cellular compositions of the pig brain(PB)remain elusive.Here we investig... The brain of the domestic pig(Sus scrofa domesticus)has drawn considerable attention due to its high similarities to that of humans.However,the cellular compositions of the pig brain(PB)remain elusive.Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB(frontal lobe,parietal lobe,temporal lobe,occipital lobe,and hypothalamus)and identified 21 cell subpopulations.The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution.Furthermore,we identified cell types and molecular pathways closely associated with neurological disorders,bridging the gap between gene mutations and pathogenesis.We reported,to our knowledge,the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species,providing extensive resources for future research regarding neural science,evolutionary developmental biology,and regenerative medicine. 展开更多
关键词 Domestic pig Brain cell atlas Single-cell transcriptome Neural science
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