目的探讨神经纤毛蛋白-1(NRP-1)在皮肤黑色素瘤中细胞株和肿瘤组织中的表达及NRP-1单克隆抗体对皮肤黑色素瘤中细胞株增殖和凋亡的影响。方法 Western blot检测各细的NRP-1表达情况。免疫组化法检测NRP-1蛋白在人正常皮肤组织和皮肤黑...目的探讨神经纤毛蛋白-1(NRP-1)在皮肤黑色素瘤中细胞株和肿瘤组织中的表达及NRP-1单克隆抗体对皮肤黑色素瘤中细胞株增殖和凋亡的影响。方法 Western blot检测各细的NRP-1表达情况。免疫组化法检测NRP-1蛋白在人正常皮肤组织和皮肤黑色素瘤中的表达;CCK-8试剂盒检测NRP-1单克隆抗体对SK-MEL-1细胞株增殖的影响;流式细胞术检测NRP-1单克隆抗体对SK-MEL-1细胞株凋亡的影响。结果 Western blot检测结果显示,NRP-1在4种人黑色素瘤细胞株中的表达量均明显高于人正常皮肤黑色素细胞株(P<0.05)。免疫组化结果提示,NRP-1在6例正常皮肤组织平均表达指数为0.33±0.52;12例皮肤黑色素瘤组织平均表达指数为7.32±1.68(P <0.05)。细胞增殖实验结果提示,低、中、高浓度组的细胞相对增殖活性均<100%,即低、中、高浓度的NRP-1单克隆抗体均对细胞增殖有抑制作用。流式细胞术结果提示PBS组的细胞凋亡率为(1.43±0.15)%,低浓度组细胞凋亡率为(5.57±1.44)%;终浓度组细胞凋亡率为(13.86±3.84)%,高浓度组细胞凋亡率为(36.03±7.51)%。抗体处理组的细胞凋亡率均较PBS组高(P<0.05)。结论 NRP-1在人皮肤黑色素瘤细胞株和肿瘤组织中均有高表达。NRP-1单克隆抗体可显著抑制人皮肤黑色素瘤细胞株的增殖和促进细胞凋亡。展开更多
Background: There is a dearth of information regarding the occurrence of neurocutaneous melanocytosis (NCM) in a large cohort of persons with large congenital melanocytic nevi (LMCN) or multiple congenital melanocytic...Background: There is a dearth of information regarding the occurrence of neurocutaneous melanocytosis (NCM) in a large cohort of persons with large congenital melanocytic nevi (LMCN) or multiple congenital melanocytic nevi (MCMN). Objective: The purpose of this article is to report occurrence of NCM and other complications in 1008 persons having LCMN or MCMN. Methods: Evaluation of information obtained from a database of persons with LCMN or MCMN voluntarily submitted by the affected persons to a nevus support group, the Nevus Network. Results: Of those with truncal LCMN, 6.8% developed significant complications, 4.8% developed symptomatic NCM, and 2.3% died from either benign or malignant NCM or cutaneous melanoma. Of the 4.8% of persons with a truncal nevus who developed symptomatic NCM, 34% died. Of those with head or extremity LCMN, 0.8% developed symptomatic NCM, and, to date, none have died from any cause. Of the small number with MCMN without a giant nevus, 71% developed symptomatic NCM, and 41% died of it. Limitations: Attending physician confirmation of submitted information was unavailable. Conclusions: LCMN of the trunk were associated with a relatively low occurrence of medical complications and death in our group, considering the large nevomelanocytic burden present. If symptomatic NCM developed in those with truncal nevi, the occurrence of death rose to a third. LCMN of the head or extremity were associated with minimal medical complications and no deaths. In contrast, most of the rare persons (N = 17) with MCMN developed symptomatic NCM, and more than a third died.展开更多
We report two atypical cases of membranous aplasia cutis surrounded by a rim of hairs, one case associated with dense dermal melanocytosis and the other with naevus flammeus, with characteristic clinical features. A r...We report two atypical cases of membranous aplasia cutis surrounded by a rim of hairs, one case associated with dense dermal melanocytosis and the other with naevus flammeus, with characteristic clinical features. A rim of hypertrichosis, ‘hair collar’sign, is proposed to have a close association with neuroectodermal defects. A failure of the normal closure of the cranial neural tube might have affected foetal skin development, including melanoblast migration and capillary network formation. The changes in the present cases, as well as the hair collar sign may suggest a complex hamartomatous nature of membranous aplasia cutis.展开更多
Background: Neurofibromatosis, type 1, is associated with cutaneous melanin pigmentation, but an association with ordinary melanocytic nevi has not been described. Methods: This retrospective case-control study was de...Background: Neurofibromatosis, type 1, is associated with cutaneous melanin pigmentation, but an association with ordinary melanocytic nevi has not been described. Methods: This retrospective case-control study was designed to see if neurofibromas in patients with neurofibromatosis, type 1 (NF-1) differ from sporadic neurofibromas (SN) in their incidence of associated melanocytic nevi and other histologic features. Slides from 114 NF-1 were compared with 112 SN and 300 intradermal melanocytic nevi (IDN). Results: Small lentiginous melanocytic nevi were identified over 13 NF-1 (11%) but no SN (P=0.0002). Compared with other NF-1, NF-1 with nevi were more frequently associated with melanocytic hyperplasia, giantmelanosomes and diffuse neurofibroma (P < 0.03). Compared with SN, NF-1 were also more frequently assoc iated with melanocytic hyperplasia, lentigo simplex-like changes, diffuse neuro fibroma and plexiform neurofibroma (P < 0.001). Sebaceous hyperplasia (14%), de rmal elastosis (9%), lipomatous change (8%), epithelial cysts (4%) and kerati n granulomas or folliculitis (3%) were not significantly different in prevalenc e between NF-1, SN and the control group of IDN. Conclusions: This study sugges ts that there is a difference in the potential for melanocytic proliferation in NF-1 compared with SN. NF-1, SN and IDN are associated with a similar range of incidental histologic changes.展开更多
文摘目的探讨神经纤毛蛋白-1(NRP-1)在皮肤黑色素瘤中细胞株和肿瘤组织中的表达及NRP-1单克隆抗体对皮肤黑色素瘤中细胞株增殖和凋亡的影响。方法 Western blot检测各细的NRP-1表达情况。免疫组化法检测NRP-1蛋白在人正常皮肤组织和皮肤黑色素瘤中的表达;CCK-8试剂盒检测NRP-1单克隆抗体对SK-MEL-1细胞株增殖的影响;流式细胞术检测NRP-1单克隆抗体对SK-MEL-1细胞株凋亡的影响。结果 Western blot检测结果显示,NRP-1在4种人黑色素瘤细胞株中的表达量均明显高于人正常皮肤黑色素细胞株(P<0.05)。免疫组化结果提示,NRP-1在6例正常皮肤组织平均表达指数为0.33±0.52;12例皮肤黑色素瘤组织平均表达指数为7.32±1.68(P <0.05)。细胞增殖实验结果提示,低、中、高浓度组的细胞相对增殖活性均<100%,即低、中、高浓度的NRP-1单克隆抗体均对细胞增殖有抑制作用。流式细胞术结果提示PBS组的细胞凋亡率为(1.43±0.15)%,低浓度组细胞凋亡率为(5.57±1.44)%;终浓度组细胞凋亡率为(13.86±3.84)%,高浓度组细胞凋亡率为(36.03±7.51)%。抗体处理组的细胞凋亡率均较PBS组高(P<0.05)。结论 NRP-1在人皮肤黑色素瘤细胞株和肿瘤组织中均有高表达。NRP-1单克隆抗体可显著抑制人皮肤黑色素瘤细胞株的增殖和促进细胞凋亡。
文摘Background: There is a dearth of information regarding the occurrence of neurocutaneous melanocytosis (NCM) in a large cohort of persons with large congenital melanocytic nevi (LMCN) or multiple congenital melanocytic nevi (MCMN). Objective: The purpose of this article is to report occurrence of NCM and other complications in 1008 persons having LCMN or MCMN. Methods: Evaluation of information obtained from a database of persons with LCMN or MCMN voluntarily submitted by the affected persons to a nevus support group, the Nevus Network. Results: Of those with truncal LCMN, 6.8% developed significant complications, 4.8% developed symptomatic NCM, and 2.3% died from either benign or malignant NCM or cutaneous melanoma. Of the 4.8% of persons with a truncal nevus who developed symptomatic NCM, 34% died. Of those with head or extremity LCMN, 0.8% developed symptomatic NCM, and, to date, none have died from any cause. Of the small number with MCMN without a giant nevus, 71% developed symptomatic NCM, and 41% died of it. Limitations: Attending physician confirmation of submitted information was unavailable. Conclusions: LCMN of the trunk were associated with a relatively low occurrence of medical complications and death in our group, considering the large nevomelanocytic burden present. If symptomatic NCM developed in those with truncal nevi, the occurrence of death rose to a third. LCMN of the head or extremity were associated with minimal medical complications and no deaths. In contrast, most of the rare persons (N = 17) with MCMN developed symptomatic NCM, and more than a third died.
文摘We report two atypical cases of membranous aplasia cutis surrounded by a rim of hairs, one case associated with dense dermal melanocytosis and the other with naevus flammeus, with characteristic clinical features. A rim of hypertrichosis, ‘hair collar’sign, is proposed to have a close association with neuroectodermal defects. A failure of the normal closure of the cranial neural tube might have affected foetal skin development, including melanoblast migration and capillary network formation. The changes in the present cases, as well as the hair collar sign may suggest a complex hamartomatous nature of membranous aplasia cutis.
文摘Background: Neurofibromatosis, type 1, is associated with cutaneous melanin pigmentation, but an association with ordinary melanocytic nevi has not been described. Methods: This retrospective case-control study was designed to see if neurofibromas in patients with neurofibromatosis, type 1 (NF-1) differ from sporadic neurofibromas (SN) in their incidence of associated melanocytic nevi and other histologic features. Slides from 114 NF-1 were compared with 112 SN and 300 intradermal melanocytic nevi (IDN). Results: Small lentiginous melanocytic nevi were identified over 13 NF-1 (11%) but no SN (P=0.0002). Compared with other NF-1, NF-1 with nevi were more frequently associated with melanocytic hyperplasia, giantmelanosomes and diffuse neurofibroma (P < 0.03). Compared with SN, NF-1 were also more frequently assoc iated with melanocytic hyperplasia, lentigo simplex-like changes, diffuse neuro fibroma and plexiform neurofibroma (P < 0.001). Sebaceous hyperplasia (14%), de rmal elastosis (9%), lipomatous change (8%), epithelial cysts (4%) and kerati n granulomas or folliculitis (3%) were not significantly different in prevalenc e between NF-1, SN and the control group of IDN. Conclusions: This study sugges ts that there is a difference in the potential for melanocytic proliferation in NF-1 compared with SN. NF-1, SN and IDN are associated with a similar range of incidental histologic changes.
