目的 探讨大鼠福尔马林致痛的可能机制。方法 雄性 SD大鼠 64只 ,随机分为对照组 ( NS组、福尔马林对照组 )和实验组 ( L a F组、L n F组 )。 NS组为正常对照组 ,L a F组、L n F组在同福尔马林对照组处理前分别给予 L -精氨酸 ( L - A...目的 探讨大鼠福尔马林致痛的可能机制。方法 雄性 SD大鼠 64只 ,随机分为对照组 ( NS组、福尔马林对照组 )和实验组 ( L a F组、L n F组 )。 NS组为正常对照组 ,L a F组、L n F组在同福尔马林对照组处理前分别给予 L -精氨酸 ( L - Arg) ,L - NG-硝基精氨酸甲酯 ( L - NAME)鞘内注射 ,在福尔马林处理后 1/ 2 h、0～ 1h分别检测大鼠脊髓 NOS及 Fos的表达及观察大鼠的行为学表现。结果 福尔马林对照组的缩腿舔爪时间长于 NS组、脊髓的NOS及 Fos表达显著强于 NS组 ,预先给予 L - Arg或 L - NAME分别能加强或抑制以上作用。结论 福尔马林致痛能引起大鼠脊髓背角 NO的释放 ,诱导展开更多
目的：探讨坐骨神经旁注射右美托咪啶对福尔马林致痛小鼠痛行为学及脊髓c-fos表达的影响。方法雄性成年昆明小鼠32只，体质量20～25 g ，随机分为4组，每组8只。A组在小鼠右侧坐骨神经旁注射生理盐水；B组在小鼠右足底皮下注射5％福尔...目的：探讨坐骨神经旁注射右美托咪啶对福尔马林致痛小鼠痛行为学及脊髓c-fos表达的影响。方法雄性成年昆明小鼠32只，体质量20～25 g ，随机分为4组，每组8只。A组在小鼠右侧坐骨神经旁注射生理盐水；B组在小鼠右足底皮下注射5％福尔马林（10μL ），建立急性炎性痛模型；C组在小鼠致痛前15 min预先在右侧坐骨神经旁注射右美托咪啶0．1μg ；D组在小鼠右侧坐骨神经旁注射右美托咪啶0．1μg。观察各组小鼠Ⅰ、Ⅱ时相舔咬足时间，在2 h时间点处死小鼠，取腰L4～5段脊髓，行免疫荧光检测c-fos的表达。结果与A组比较，B组小鼠在Ⅰ、Ⅱ时相出现明显的舔咬足行为，疼痛评分明显增高，脊髓c-fos的表达明显增多（P＜0．05）；与B组比较，C组舔咬足时间明显缩短，疼痛评分明显降低、脊髓c-fos的表达明显减少（P＜0．05）；A、C、D组疼痛评分和脊髓c-fos的表达差异无统计学意义。结论坐骨神经旁注射右美托咪啶能够减轻小鼠足底注射福尔马林引起的急性炎性痛，并且降低脊髓c-fos的表达。展开更多
目的 探讨新斯的明 (Neo)对福尔马林致痛大鼠镇痛机理。方法 雄性 SD大鼠 4 0只 ,随机分为对照组和实验组 ,对照组又分为 NS组和 F组 ,实验组又细分为 Ne F组、L a Ne F组和 L n Ne F组。 NS组为正常对照组 ,F组给予 5 %福尔马林 10 0...目的 探讨新斯的明 (Neo)对福尔马林致痛大鼠镇痛机理。方法 雄性 SD大鼠 4 0只 ,随机分为对照组和实验组 ,对照组又分为 NS组和 F组 ,实验组又细分为 Ne F组、L a Ne F组和 L n Ne F组。 NS组为正常对照组 ,F组给予 5 %福尔马林 10 0 μl,足底注射 ,Ne F组、L a Ne F组及 L n Ne F组在同 F组处理前分别给予 Neo、L-精氨酸(L- Arg) +Neo鞘内注射 (ith)、L- NG-硝基精氨酸甲酯 (L- NAME,NOS抑制剂 ) +Neo ith,在福尔马林处理后分别检测大鼠脊髓 Fos的表达及观察大鼠的行为学表现。结果 Ne F组的缩腿舔爪时间显著短于 F组 ,脊髓的 Fos表达显著弱于 F组 ,预先给予 L- Arg或 L- NAME分别能加强及抑制以上作用。结论 新斯的明能引起大鼠脊髓背角 NO的释放 ,抑制 Fos表达可能是其产生抗伤害作用的机制之一。展开更多
Aim To study the effects of tetrodotoxin （TTX） combined with acetylsalicylic acid （ASA） on nociceptive stimulus in mice. Methods To assess the antinociceptive effects of TTX, ASA or TTX plus ASA, the acetic acid-i...Aim To study the effects of tetrodotoxin （TTX） combined with acetylsalicylic acid （ASA） on nociceptive stimulus in mice. Methods To assess the antinociceptive effects of TTX, ASA or TTX plus ASA, the acetic acid-induced abdominal constriction test and formalin pain test were used. Results TTX （0.5 - 4.0 μg· kg^-1 ） or ASA （25 - 200 mg· kg^-1 ） im produced a significant inhibition of acetic acid-induced abdominal constriction. The median inhibitory doses （ID508） were 2.1 μg· kg^-1 for TTX（ and 64 mg· kg^-1 for ASA. TTX and ASA also showed a dose-dependent inhibition of the second phase response in the formalin pain model, the ID508, being 2.3μg·kg^-1 and 74.2 mg· kg^-1, respectively. The ihteraction between TTX and ASA was synergistic, as evidenced by the fact that （1） when ASA alone compared with the combination of TTX （0.79 μg · kg^-1 or 0.39μg· kg^-1 ） and ASA, the ID508, of ASA reduced from 64.0 mg· kg^-1 to 5.8 mg· kg^-1 or 12.6 mg· kg^-1, and from 74.2 mg· kg^-1 to 7.4 mg· kg^-1 or 13.0 mg· kg^-1 on tile two models of nociceptive tests, respectively; and that （2） synergism in the analgesic effects was shown by isobiolographic analysis. Conclusion TTX, ASA and the combination of the two drags produce analgesic effects in acetic acid-induced abdominal constriction test and formalin-induced pain test. The interactions between TTX and ASA may be useful in developing novel analgesic agents.展开更多
The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （MEOC） using animal models of nociception and inflam...The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （MEOC） using animal models of nociception and inflammation. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Oral administration of MEOC （500 mg/kg） produced significant （t7 〈 0.05） antinociceptive effects when tested in mice using acetic acid-induced abdominal writhing test and on the inflammatory phase of the formalin test. It was also demonstrated that MEOC had no significant effect on the response latency time to the heat stimulus in the thermal model of the hot plate test. The anti-inflammatory activity of the extract was assessed using carrageenan, histamine and serotonin induced oedema in rat paw. The oral administration of MEOC showed maximum inhibition （64.29%） at 1 h on carrageenan edema, but it did not modify the edema induced by histamine and serotonin. The present results suggest that MEOC has a peripheral antinoeiceptive and anti-inflammatory action.展开更多
文摘Aim To study the effects of tetrodotoxin （TTX） combined with acetylsalicylic acid （ASA） on nociceptive stimulus in mice. Methods To assess the antinociceptive effects of TTX, ASA or TTX plus ASA, the acetic acid-induced abdominal constriction test and formalin pain test were used. Results TTX （0.5 - 4.0 μg· kg^-1 ） or ASA （25 - 200 mg· kg^-1 ） im produced a significant inhibition of acetic acid-induced abdominal constriction. The median inhibitory doses （ID508） were 2.1 μg· kg^-1 for TTX（ and 64 mg· kg^-1 for ASA. TTX and ASA also showed a dose-dependent inhibition of the second phase response in the formalin pain model, the ID508, being 2.3μg·kg^-1 and 74.2 mg· kg^-1, respectively. The ihteraction between TTX and ASA was synergistic, as evidenced by the fact that （1） when ASA alone compared with the combination of TTX （0.79 μg · kg^-1 or 0.39μg· kg^-1 ） and ASA, the ID508, of ASA reduced from 64.0 mg· kg^-1 to 5.8 mg· kg^-1 or 12.6 mg· kg^-1, and from 74.2 mg· kg^-1 to 7.4 mg· kg^-1 or 13.0 mg· kg^-1 on tile two models of nociceptive tests, respectively; and that （2） synergism in the analgesic effects was shown by isobiolographic analysis. Conclusion TTX, ASA and the combination of the two drags produce analgesic effects in acetic acid-induced abdominal constriction test and formalin-induced pain test. The interactions between TTX and ASA may be useful in developing novel analgesic agents.
文摘The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （MEOC） using animal models of nociception and inflammation. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Oral administration of MEOC （500 mg/kg） produced significant （t7 〈 0.05） antinociceptive effects when tested in mice using acetic acid-induced abdominal writhing test and on the inflammatory phase of the formalin test. It was also demonstrated that MEOC had no significant effect on the response latency time to the heat stimulus in the thermal model of the hot plate test. The anti-inflammatory activity of the extract was assessed using carrageenan, histamine and serotonin induced oedema in rat paw. The oral administration of MEOC showed maximum inhibition （64.29%） at 1 h on carrageenan edema, but it did not modify the edema induced by histamine and serotonin. The present results suggest that MEOC has a peripheral antinoeiceptive and anti-inflammatory action.
