快律宁胶囊对外源性自由基所致心律失常的影响

目的:研究快律宁胶囊(KLN)对外源性自由基所致心律失常的影响,对比研究KLN与维拉帕米对其的保护作用。方法:将SPF级SD大鼠分为对照组,维拉帕米组和KLN胶囊高、中、低剂量组,采用Langendoff灌流装置对大鼠离体心脏灌注硫酸亚铁/抗坏血酸的方法,复制外源性自由基所致大鼠心律失常模型,对比研究KLN胶囊与维拉帕米对其的保护作用。结果:与对照组相比,KLN胶囊可以剂量依赖地减少对外源性自由基所诱发室性早搏(VEB)、室速(VT)的发生次数(P<0.01);KLN胶囊3个剂量组及维拉帕米组均未出现室颤(VF),而对照组则诱发出VF,但差异无统计学意义(P>0.05);与对照组相比,KLN胶囊高剂量组心律失常的出现时间较晚(P<0.05),而KLN胶囊中低剂量组、维拉帕米组心律失常的出现也有推迟的趋势,但差异无统计学意义(P>0.05);与对照组相比,KLN胶囊3个剂量组的心率明显减慢,而且呈剂量相关性;与对照组性比,KLN胶囊高、中剂量组的心律失常评分明显降低(P<0.05),而KLN胶囊低剂量组和维拉帕米组则与对照组比较差异无统计学意义(P>0.05)。结论:KLN胶囊对外源性自由基诱发的心律失常可能具有预防和治疗作用,可以明显减慢心率,并可能对恶性心律失常(VT、VF)的发生具有一定的预防作用,其疗效优于维拉帕米。

A pharmacodynamic model of portal hypertension in isolated perfused rat liver

AIM： To develop a pharmacodynamic model of porta hypertension from chronic hepatitis. METHODS： Pathological changes and collagen depositions were analyzed using morphometry to confirm CCI4-induced chronic hepatitis. At do, d28, ds6 and d84 of the process, the portal perfused velocities （μL/min） in isolated rat livers were exactly controlled with a quanti-fied pump. The pressure （mmHg） was monitored with a Physiological System. The geometric concentrations of phenylephrine or acetylcholine were added to a fixed volume （300 mL） of the circulating perfusate. The equation, the median effective concentration and its 95% confidence intervals of phenylephrine or acetyl- choline were regressed with Prism-4 software in non-linear fit and various slopes. In the isolated perfused rat livers with chronic hepatitis, both median effective concentrations were defined as the pharmacodynamic model of portal hypertension.CONCLUSION： A pharmacodynamic model of portal hypertension in isolated perfused rat livers with chronic hepatitis was defined as the median effective concen- trations of phenylephrine and acetylcholine.