离体鼠皮苯甲酸雌二醇凝胶透皮吸收试验

目的 :验证苯甲酸雌二醇凝胶透皮吸收性能。方法 :采用自制实验装置 ,用离体鼠皮 ,高效液相色谱法测定释放液及接受液中苯甲酸雌二醇含量。结果 :3 0min时释放液中苯甲酸雌二醇含量即有明显下降 ,随后下降率变化趋缓 ;60min时开始在接受液中测出苯甲酸雌二醇 ,增加率随时间不断增长。释放液中苯甲酸雌二醇含量下降率变化趋势与接受液中其含量增加率的变化趋势不呈正相关。结论 :本试验方法简便易行 。

咪康唑莫米松皮肤成膜凝胶的体外透皮研究

目的：观察咪康唑莫米松皮肤成膜凝胶的体外透皮性能。方法：采用HPLC-DAD法测定咪康唑莫米松的含量,用Franz扩散池以离体鼠皮为透皮屏障进行体外透皮扩散试验,并计算咪康唑莫米松药物累积透皮量Q（Q咪和Q莫）和透皮速率常数J（J咪和J莫）。结果：咪康唑与莫米松的色谱峰能有效分离,进样量分别在1.0~400.0μg·ml-1和0.1~40.0μg·ml-1范围与各自峰面积呈良好线性关系,r值分别为0.9988和0.9982（n=6）,咪康唑与莫米松的J值分别为（17.810±0.558）μg·cm-2和（1.821±0.941）μg·cm-2·h-1,r值分别为0.9913和0.9908,无显著差异（P≥0.05）,咪康唑莫米松的透皮吸收行为均符合零级动力学方程。结论：咪康唑莫米松皮肤成膜凝胶,可开发为经皮给药制剂。

The Effect of pH on the Permeation of Lidocaine Hydrochloride Across Excised Rat Skin

The effect of pH on the permeation of Lidocaine hydrochloride (LH) across excised rat skin was studied, the steady state flux (JSS) at different pH being determined using improved Valia-Chien diffusion cells. JSS increased substantially when pH was close to the pKa of LH. The profile of JSS versus pH showed an 慡?shaped curve. JSS of Lidocaine free base (LFB) was fourteen times that of LH. The pH of vehicle influences the permeation of LH significantly and should be considered as an important factor when a formulation is developed.