In order to improve the in vitro fertilization rate of bovine, the effects of different sperm capacitation methods on fertilization were investigated. Total two treatments were designed： two-time centrifugation （C）...In order to improve the in vitro fertilization rate of bovine, the effects of different sperm capacitation methods on fertilization were investigated. Total two treatments were designed： two-time centrifugation （C）, and one-time centrifugation and swim-up method （CS）. The results showed that the cleavage rate in the C treatment group was （75.6±4.5）%, which showed no difference compared with that （（76.4±1.9）%） in the CS treatment group （P〉0.05）; there was also no significant dif- ference in blastocyst rate between the two treatment groups （（35.7±4.1）% vs. （36.3± 2.7）%, P〉0.05）. However, the CS treatment significantly saved the capacitation time in vitro.展开更多
基金Supported by Earmarked Fund for China Agriculture(Beef and Yak)Research System(NYCYTX-38)Key Agriculture Research and Development Program of Qiqihar(NYGG-201524)~~
文摘In order to improve the in vitro fertilization rate of bovine, the effects of different sperm capacitation methods on fertilization were investigated. Total two treatments were designed： two-time centrifugation （C）, and one-time centrifugation and swim-up method （CS）. The results showed that the cleavage rate in the C treatment group was （75.6±4.5）%, which showed no difference compared with that （（76.4±1.9）%） in the CS treatment group （P〉0.05）; there was also no significant dif- ference in blastocyst rate between the two treatment groups （（35.7±4.1）% vs. （36.3± 2.7）%, P〉0.05）. However, the CS treatment significantly saved the capacitation time in vitro.
