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《莺莺传》研究百年回顾 被引量:14
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作者 胥洪泉 《涪陵师范学院学报》 2006年第1期50-55,共6页
上个世纪的前五十年,《莺莺传》研究较为冷寂,研究不够广泛、深入,方法单一,主要是鲁迅赞同的北宋王铚所谓“张生即元稹自寓”的观点,影响较大。后五十年,《莺莺传》研究热闹起来,研究的视野拓宽、方法多样,对《莺莺传》的创作时间、张... 上个世纪的前五十年,《莺莺传》研究较为冷寂,研究不够广泛、深入,方法单一,主要是鲁迅赞同的北宋王铚所谓“张生即元稹自寓”的观点,影响较大。后五十年,《莺莺传》研究热闹起来,研究的视野拓宽、方法多样,对《莺莺传》的创作时间、张生和崔莺莺的原型、崔张离异的原因等问题,进行了深入的探讨和激烈的争论,取得了可喜的成绩。 展开更多
关键词 《莺莺传》 创作时间 崔张原型 离异原因
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Isolation and identification of a sulfate reducing bacteria and sequence analysis of its dissimilatory sulfite reductase gene 被引量:1
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作者 魏利 马放 +3 位作者 魏继承 李艳萍 SHAIK FIRDOZ 吕晓磊 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2009年第6期854-858,共5页
A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and... A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and nitrogen source. The sequence analysis of 16S rDNA illustrated that the similarity of F8 and Desulfovibrio desulfuricans (AF192153) was 99%, and the similarity sequence of dissimilatory sulfite reductase gene (DSR) cloned from the strain and Desulfovibrio desulfuricans (AF273034) was 98%. Their phylogenitic analysis was basically anastomosed, and thus temporarily named as Desulfovibrio desulfuricans F8. The DSR cloned from F8 strain was 2740 bp in length consisting of three ORF, DSRA, DSRB and DSRD as a single operon (DSRABD) regulated by the same operator. DSRA contained typical conservative box of sulfate—sulfite reducing enzyme (SiteⅠand SiteⅡ), which could bind siroheme and [Fe4S4]. DSRB retained a [Fe4S4] binding site, with an uncomplimentary structure for siroheme binding. There was no conservative box in DSRD. Sequence analysis of DSR will provide a theoretical basis for quantitative detection, metabolic pathway modification through gene engineering, and sulfate reducing bacteria (SRB) suppression. 展开更多
关键词 sulfate reducing bacteria DSR 16S rDNA sequence DSRABD zene sequence analysis
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