禽/人流感防控项目县目标人群相关核心知识知晓情况分析

目的:了解禽/人流感防控项目县目标人群相关核心知识知晓情况,为针对性开展禽/人流感干预活动提供依据。方法:在两个项目县随机抽取9个村作为样本点,并整群抽取12-65岁的禽类养殖人员和附近居民作为目标人群进行问卷调查,所有数据采用SPSS12.0进行统计分析。结果:874名被调查者的核心知识总知晓率为59.10%,但"发现病死的家禽要深埋并上报"和"禽/人流感有多种传播途径"的回答正确率低于10.00%;影响目标人群核心知识知晓情况的因素有年龄、文化程度和学习禽/人流感知识的态度;目标人群知识来源主要为电视,而宣传材料和人际传播覆盖率较低。结论:项目县目标人群对禽/人流感知识有一定了解,但其知识仍缺乏系统性和全面性,应通过多种途径对目标人群进行有针对性的健康教育。

Conservation of T cell epitopes between seasonal influenza viruses and the novel influenza A H7N9 virus

A novel avian influenza A(H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as H5N1 and H9N2. While there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved T-cell epitopes between endemic H1N1 and H3N2 influenza viruses and the novel H7N9 virus contributes to this observation. Here we demonstrate that a number of T cell epitopes are conserved between endemic H1N1 and H3N2 viruses and H7N9 virus. Most of these conserved epitopes are from viral internal proteins. The extent of conservation between endemic human seasonal influenza and avian influenza H7N9 was comparable to that with the highly pathogenic avian influenza H5N1. Thus, the ease of inter-species transmission of H7N9 viruses(compared with avian H5N1 viruses) cannot be attributed to the lack of conservation of such T cell epitopes. On the contrary, our findings predict significant T-cell based cross-reactions in the human population to the novel H7N9 virus. Our findings also have implications for H7N9 virus vaccine design.

The spreading frontiers of avian-human influenza described by the free boundary

In this paper,a reaction-diffusion system is proposed to investigate avian-human influenza.Two free boundaries are introduced to describe the spreading frontiers of the avian influenza.The basic reproduction numbers rF0(t)and RF0(t)are defined for the bird with the avian influenza and for the human with the mutant avian influenza of the free boundary problem,respectively.Properties of these two time-dependent basic reproduction numbers are obtained.Sufficient conditions both for spreading and for vanishing of the avian influenza are given.It is shown that if rF0(0)<1 and the initial number of the infected birds is small,the avian influenza vanishes in the bird world.Furthermore,if rF0(0)<1 and RF0(0)<1,the avian influenza vanishes in the bird and human worlds.In the case that rF0(0)<1 and RF0(0)>1,spreading of the mutant avian influenza in the human world is possible.It is also shown that if rF0(t0)>1 for any t0>0,the avian influenza spreads in the bird world.

Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human

A novel H7N9 influenza A virus has been discovered as the causative identity of the emerging acute respiratory infection cases in Shanghai,China.This virus has also been identified in cases of infection in the neighboring area Hangzhou City in Zhejiang Province.In this study,epidemiologic,clinical,and virological data from three patients in Hangzhou who were confirmed to be infected by the novel H7N9 influenza A virus were collected and analyzed.Human respiratory specimens and chicken feces from a contacted free market were tested for influenza virus by real-time reverse transcription PCR(RT-PCR) and sequencing.The clinical features of the three cases were similar featured with high fever and severe respiratory symptoms;however,only one of the patients died.A certain degree of diversity was observed among the three Hangzhou viruses sequenced from human samples compared with other reported H7N9 influenza A viruses.The sequences of the novel avian-origin H7N9 influenza viruses from Hangzhou City contained important amino acid substitutions related to human adaptation.One of the Hangzhou viruses had gained a novel amino acid substitution(Q226I) in the receptor binding region of hemagglutinin.More importantly,the virus sequenced from the chicken feces had a 627E substitution in the PB2 protein instead of the mammalian-adapted 627K substitution that was found in the PB2 proteins from the Hangzhou viruses from the three patients.Therefore,the newly-emerging H7N9 virus might be under adaptation pressure that will help it "jump" from avian to human hosts.The significance of these substitutions needs further exploration,with both laboratory experiments and extensive field surveillance.

Dynamical analysis of the avian-human influenza epidemic model using multistage analytical method

In this paper, analytical result of avian-human influenza epidemic model has been inves- tigated by applying homotopy analysis method （HAM） and by expanding it to hybrid numeric-analytic method which is known as multistage HAM （MSHAM）. HAM is an algorithm which gives us the approximate solution of the problem in an arrangement of time interims and by modifying it to multistage one. Some advantages such as flexibility of picking the auxiliary linear operator and the auxiliary parameter are emerged, that leads us to achieve some excellent results in this work. Furthermore, in this analyti- cal work, obtained results are compared and reported with numerical ones which were obtained previously from methods such as the Runge-Kutta （RK4） method.