通过对人、猪和鸡血清中的抗禽戊型肝炎病毒（hepatitis E virus,HEV）ORF2抗体的检测以及对阳性血清抗体特异性的鉴定,本研究结果表明,人、猪和禽血清中抗禽HEVORF2抗体阳性率为0.91%~62.5%。因为禽与哺乳动物HEV ORF2抗原具有共有的...通过对人、猪和鸡血清中的抗禽戊型肝炎病毒（hepatitis E virus,HEV）ORF2抗体的检测以及对阳性血清抗体特异性的鉴定,本研究结果表明,人、猪和禽血清中抗禽HEVORF2抗体阳性率为0.91%~62.5%。因为禽与哺乳动物HEV ORF2抗原具有共有的抗原表位,而阳性血清中抗禽HEV ORF2特异性抗原表位抗体的阳性率为41.3%~92.1%,提示人、猪和鸡都有感染禽HEV的可能性,这为进一步验证禽HEV可能造成的人兽共感染提供新的科学依据。展开更多
Objectives -Hepatitis E virus (HEV) is responsible for acute hepatitis predominantly in developping countries. In Western Europe and in the US, cases of acute HEV infection are uncommon and occur primarily in traveler...Objectives -Hepatitis E virus (HEV) is responsible for acute hepatitis predominantly in developping countries. In Western Europe and in the US, cases of acute HEV infection are uncommon and occur primarily in travelers returning from endemic countries. The aim of this study was to describe patients with acute hepatitis E in South West France and compare them with patients with acute hepatitis A. Methods -23 consecutive patients over 13 months were analysed. Acute hepatitis E was diagnosed on the presence of specific serum antibodies or viral RNA detection in serum or stools. Real time PCR products from viraemic patients were sequenced. Results -All the HEV sequences belonged to genotype 3. Two patients (8%) died during their hospital stay, both suffered from severe underlying disease. Only 3 patients (13%) had travelled outside of Europe, within 3 months of the onset of disease. When compared to 23 patients with acute hepatitis A at the same hospital and during the same time frame, HEV-infected patients were older (54.4 ±16.6 vs 24.5 ±16.6, P < 0.05), had lower ALT levels (55.4 ×upper normal limit±48.6 vs 107.8 ×upper normal limit ±82.8, P < 0.05) and had lower incidence of recent travel outside of Europe (13%in the hepatitis E group vs 60%in the hepatitis A group, P < 0.05). Conclusions -Hepatitis E can be considered an autochthonous infection in South West France. All strains sequenced were related to genotype Ⅲ. When compared to hepatitis A, HEV-infected patients were older, had lower ALT levels and had a lower incidence of travel outside of Europe.展开更多
戊型肝炎病毒（Hepatitis E virus,HEV）感染是包括我国在内发展中国家成人急性肝炎的主要原因,在发达国家戊型肝炎的发病率也在不断升高,且在免疫抑制患者中可引起慢性感染,因此对HEV感染的诊断受到广泛重视。血清学检测,包括抗-HEV Ig...戊型肝炎病毒（Hepatitis E virus,HEV）感染是包括我国在内发展中国家成人急性肝炎的主要原因,在发达国家戊型肝炎的发病率也在不断升高,且在免疫抑制患者中可引起慢性感染,因此对HEV感染的诊断受到广泛重视。血清学检测,包括抗-HEV IgM和IgG,仍是诊断HEV感染的主要手段,但目前存在的最大问题是检测抗-HEV抗体的试剂之间的敏感性和特异性差异很大,检测结果的符合率差。本文着重综述了国内外常用抗-HEV抗体检测试剂的组成以及检测结果,提出了今后可能有利于提高检测敏感性和特异性的研究构思。展开更多
戊型肝炎是一种人畜共患病，猪为主要存储宿主和传染源。人类可能通过摄入生猪肝脏而感染戊型肝炎病毒（hepatitis E virus，HEV）。明确HEV在养猪场传播的精细机制有助于理解HEV感染，特别是在屠宰之前感染的年龄别模式。本文对日本养...戊型肝炎是一种人畜共患病，猪为主要存储宿主和传染源。人类可能通过摄入生猪肝脏而感染戊型肝炎病毒（hepatitis E virus，HEV）。明确HEV在养猪场传播的精细机制有助于理解HEV感染，特别是在屠宰之前感染的年龄别模式。本文对日本养猪场的一项大规模血清流行病学调查数据进行再分析，探讨HEV在猪群中的传播动力学。感染力（force of infection）以年龄别血清特异性抗体流行率表示，基本再生数（basic reproduction number）定义为一个初级感染个体引起的次级感染个体的平均数。展开更多
文摘通过对人、猪和鸡血清中的抗禽戊型肝炎病毒（hepatitis E virus,HEV）ORF2抗体的检测以及对阳性血清抗体特异性的鉴定,本研究结果表明,人、猪和禽血清中抗禽HEVORF2抗体阳性率为0.91%~62.5%。因为禽与哺乳动物HEV ORF2抗原具有共有的抗原表位,而阳性血清中抗禽HEV ORF2特异性抗原表位抗体的阳性率为41.3%~92.1%,提示人、猪和鸡都有感染禽HEV的可能性,这为进一步验证禽HEV可能造成的人兽共感染提供新的科学依据。
文摘Objectives -Hepatitis E virus (HEV) is responsible for acute hepatitis predominantly in developping countries. In Western Europe and in the US, cases of acute HEV infection are uncommon and occur primarily in travelers returning from endemic countries. The aim of this study was to describe patients with acute hepatitis E in South West France and compare them with patients with acute hepatitis A. Methods -23 consecutive patients over 13 months were analysed. Acute hepatitis E was diagnosed on the presence of specific serum antibodies or viral RNA detection in serum or stools. Real time PCR products from viraemic patients were sequenced. Results -All the HEV sequences belonged to genotype 3. Two patients (8%) died during their hospital stay, both suffered from severe underlying disease. Only 3 patients (13%) had travelled outside of Europe, within 3 months of the onset of disease. When compared to 23 patients with acute hepatitis A at the same hospital and during the same time frame, HEV-infected patients were older (54.4 ±16.6 vs 24.5 ±16.6, P < 0.05), had lower ALT levels (55.4 ×upper normal limit±48.6 vs 107.8 ×upper normal limit ±82.8, P < 0.05) and had lower incidence of recent travel outside of Europe (13%in the hepatitis E group vs 60%in the hepatitis A group, P < 0.05). Conclusions -Hepatitis E can be considered an autochthonous infection in South West France. All strains sequenced were related to genotype Ⅲ. When compared to hepatitis A, HEV-infected patients were older, had lower ALT levels and had a lower incidence of travel outside of Europe.
文摘戊型肝炎病毒（Hepatitis E virus,HEV）感染是包括我国在内发展中国家成人急性肝炎的主要原因,在发达国家戊型肝炎的发病率也在不断升高,且在免疫抑制患者中可引起慢性感染,因此对HEV感染的诊断受到广泛重视。血清学检测,包括抗-HEV IgM和IgG,仍是诊断HEV感染的主要手段,但目前存在的最大问题是检测抗-HEV抗体的试剂之间的敏感性和特异性差异很大,检测结果的符合率差。本文着重综述了国内外常用抗-HEV抗体检测试剂的组成以及检测结果,提出了今后可能有利于提高检测敏感性和特异性的研究构思。
文摘戊型肝炎是一种人畜共患病，猪为主要存储宿主和传染源。人类可能通过摄入生猪肝脏而感染戊型肝炎病毒（hepatitis E virus，HEV）。明确HEV在养猪场传播的精细机制有助于理解HEV感染，特别是在屠宰之前感染的年龄别模式。本文对日本养猪场的一项大规模血清流行病学调查数据进行再分析，探讨HEV在猪群中的传播动力学。感染力（force of infection）以年龄别血清特异性抗体流行率表示，基本再生数（basic reproduction number）定义为一个初级感染个体引起的次级感染个体的平均数。
