Root zone N management can reduce N fertilizer input and avoid nitrate pollution in greenhouse tomato production; however, little information is available on how reduced N affects soil microbes, especially dominant ba...Root zone N management can reduce N fertilizer input and avoid nitrate pollution in greenhouse tomato production; however, little information is available on how reduced N affects soil microbes, especially dominant bacteria populations in long-term continuous greenhouse systems. This study investigated the effects of different combinations of N and straw on the population size of soil total bacteria and two dominant bacteria （Pseudomonas spp. andBacilusspp.）, and microbial community structure in a 6-year greenhouse tomato trial. The treatments were basal fertilizer （10 t ha-1 chicken manure; control）, conventional high N treatment [600 kg N ha-1], conventional high N treatment with wheat straw, reduced N treatment [300 kg N ha-1 less than conventional], and reduced N treatment with wheat straw. Six years of reduced mineral N fertilization did not lead to a decrease in fruit yield. Both mineral N fertilizer and straw increased the numbers of soil bacteria （Bacilus spp. andPseudomonas spp.） compared with the control, but the numbers were not affected by the amount of mineral N fertilizer （highvs reduced）. Different denaturing gradient gel electrophoresis patterns were evidence that soil bacterial communities were changed by N treatments and straw application, but little difference in bacterial diversity was detected between high N and reduced N treatments. These results indicated that a reduction in N fertilizer input was possible in greenhouse tomato production and was beneifcial in sustainable agriculture.展开更多
基金National High-tech Research and Development Program of China(2013AA102903)
文摘Root zone N management can reduce N fertilizer input and avoid nitrate pollution in greenhouse tomato production; however, little information is available on how reduced N affects soil microbes, especially dominant bacteria populations in long-term continuous greenhouse systems. This study investigated the effects of different combinations of N and straw on the population size of soil total bacteria and two dominant bacteria （Pseudomonas spp. andBacilusspp.）, and microbial community structure in a 6-year greenhouse tomato trial. The treatments were basal fertilizer （10 t ha-1 chicken manure; control）, conventional high N treatment [600 kg N ha-1], conventional high N treatment with wheat straw, reduced N treatment [300 kg N ha-1 less than conventional], and reduced N treatment with wheat straw. Six years of reduced mineral N fertilization did not lead to a decrease in fruit yield. Both mineral N fertilizer and straw increased the numbers of soil bacteria （Bacilus spp. andPseudomonas spp.） compared with the control, but the numbers were not affected by the amount of mineral N fertilizer （highvs reduced）. Different denaturing gradient gel electrophoresis patterns were evidence that soil bacterial communities were changed by N treatments and straw application, but little difference in bacterial diversity was detected between high N and reduced N treatments. These results indicated that a reduction in N fertilizer input was possible in greenhouse tomato production and was beneifcial in sustainable agriculture.
