建立了稻虾种养系统土壤环境中稻瘟灵残留量的检测方法。采用改进的QuEChERS法处理样品,样品溶液经过在线凝胶色谱(GPC)进一步净化后直接进入气相色谱-三重四极杆质谱(gas chromatographytandem mass spectrometry,GC-MS/MS)联用仪检测...建立了稻虾种养系统土壤环境中稻瘟灵残留量的检测方法。采用改进的QuEChERS法处理样品,样品溶液经过在线凝胶色谱(GPC)进一步净化后直接进入气相色谱-三重四极杆质谱(gas chromatographytandem mass spectrometry,GC-MS/MS)联用仪检测,采集模式为多反应监测,内标法定量。结果表明:稻瘟灵化合物在10~100 ng/mL浓度范围内线性关系良好,相关系数>0.99;回收率范围为96%~109%,相对标准偏差(relative standard deviation,RSD)为2.0%。该方法操作简单,快速准确,在线GPC能有效去除基质干扰,提高分析灵敏度,满足农药残留定量分析的要求。展开更多
The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, ...The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan展开更多
Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed)....Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed). In seaweed-based integrated aquaculture, seaweeds have the capacity to reduce the environmental impact of nitrogen-rich effluents on coastal ecosystems. Thus, selection of optimal species for such aquaculture is of great importance. The present study aimed to develop a seaweed species-selection index for selecting suitable species in seaweed-based integrated aquaculture system. The index was synthesized using available literature-based information, reference data, and physiological seaweed experiments to identify and prioritize the desired species. Undaria pinnatifida, Porphyra yezoensis and Ulva compressa scored the highest according to a seaweed-based integrated aquaculture suitability index (SASI). Seaweed species with the highest scores were adjudged to fit the integrated aquaculture systems. Despite the application of this model limited by local aquaculture environment, it is considered to be a useful tool for selecting seaweed species in IMTA.展开更多
Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carri...Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.展开更多
文摘建立了稻虾种养系统土壤环境中稻瘟灵残留量的检测方法。采用改进的QuEChERS法处理样品,样品溶液经过在线凝胶色谱(GPC)进一步净化后直接进入气相色谱-三重四极杆质谱(gas chromatographytandem mass spectrometry,GC-MS/MS)联用仪检测,采集模式为多反应监测,内标法定量。结果表明:稻瘟灵化合物在10~100 ng/mL浓度范围内线性关系良好,相关系数>0.99;回收率范围为96%~109%,相对标准偏差(relative standard deviation,RSD)为2.0%。该方法操作简单,快速准确,在线GPC能有效去除基质干扰,提高分析灵敏度,满足农药残留定量分析的要求。
基金Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.
文摘The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan
基金supported by the ‘Greenhouse Gas Emissions Reduction Using Seaweeds’ programthe Jeju Sea Grant College Program funded by the Korean Ministry of Land, Transport and Maritime Affairs, Republic of Korea
文摘Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed). In seaweed-based integrated aquaculture, seaweeds have the capacity to reduce the environmental impact of nitrogen-rich effluents on coastal ecosystems. Thus, selection of optimal species for such aquaculture is of great importance. The present study aimed to develop a seaweed species-selection index for selecting suitable species in seaweed-based integrated aquaculture system. The index was synthesized using available literature-based information, reference data, and physiological seaweed experiments to identify and prioritize the desired species. Undaria pinnatifida, Porphyra yezoensis and Ulva compressa scored the highest according to a seaweed-based integrated aquaculture suitability index (SASI). Seaweed species with the highest scores were adjudged to fit the integrated aquaculture systems. Despite the application of this model limited by local aquaculture environment, it is considered to be a useful tool for selecting seaweed species in IMTA.
基金Supported by the National Natural Science Foundation of China Granted Project(81173484)
文摘Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.
