To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic a...To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.展开更多
The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It al...The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It allows one to eliminate the plant materials （initial breeding materials and cultivars） which do not tolerate extreme temperatures and temperature changes during germination, have low water use efficiency and is intolerant to abiotic stressors all right at the seed level. It was confirmed that these genotypes also have poor t^eld emergence and initial root growth implications for further vegetation periods, mainly for over wintering and spring regeneration which has significant influence on the yield. The method also represents the tool for screening genetic resources with the resistance to the abiotic stressors and this technology process is also acceptable for other crops. The results confirm the importance of the seed and root characteristics for crop production. The deteriorating quality of soil in recent years, increasing variability of weather and long periods of drought directly support the need to intensify activities in this research. Obtained results also show the influence of seed quality characteristics on roots and above ground parts of the plant. A relationship exists also vice versa.展开更多
In Mediterranean countries forage crops and temporary grasslands are the most important supply even if severe moisture stress is common. In Italy, forage systems are various and differently located from North to South...In Mediterranean countries forage crops and temporary grasslands are the most important supply even if severe moisture stress is common. In Italy, forage systems are various and differently located from North to South of the mainland due to strong influence by rainfall distribution. Grasses and grazing cover 3.4 million ha of Italian utilized agricultural area （UAA） while alternated grassland and grass meadows cover 1.9 million ha. Most of grasslands are located in hilly and mountainous areas and are important for reducing erosion. Italy has a great longitudinal extension which accounts for a great variety of climate systems and soils： the northern regions have a humid subtropical climate and differ greatly from the south part that fits the Mediterranean climate profile. During the last 100/150 years the Italian climate has become warmer and drier showing an increase of erratic precipitation intensity. The future of breeding of forage grasses and legumes should be focused on higher nutrient use efficiencies and increased sustainability. New applications of genomics and bioinformatics will allow advanced breeding strategies. Over the past 15 years breeders have displayed a constant interest in forage species while a greater interest has risen in turfgrass varieties. Seed production of Italian herbages does not cover the requirements of the market. More specific value for cultivation and use （VCU） tests might be an effective means to improve the screening of candidate varieties. The goal is the selection of varieties able to withstand the stress of climate change, have better water and nitrogen use efficiency and resilience of vegetation cover.展开更多
文摘To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
文摘The presented work is based on laboratory testing of seed germination speed, emergence and seedling growth under different stress conditions simulated by subnormal water revel, extreme high and low temperatures. It allows one to eliminate the plant materials （initial breeding materials and cultivars） which do not tolerate extreme temperatures and temperature changes during germination, have low water use efficiency and is intolerant to abiotic stressors all right at the seed level. It was confirmed that these genotypes also have poor t^eld emergence and initial root growth implications for further vegetation periods, mainly for over wintering and spring regeneration which has significant influence on the yield. The method also represents the tool for screening genetic resources with the resistance to the abiotic stressors and this technology process is also acceptable for other crops. The results confirm the importance of the seed and root characteristics for crop production. The deteriorating quality of soil in recent years, increasing variability of weather and long periods of drought directly support the need to intensify activities in this research. Obtained results also show the influence of seed quality characteristics on roots and above ground parts of the plant. A relationship exists also vice versa.
文摘In Mediterranean countries forage crops and temporary grasslands are the most important supply even if severe moisture stress is common. In Italy, forage systems are various and differently located from North to South of the mainland due to strong influence by rainfall distribution. Grasses and grazing cover 3.4 million ha of Italian utilized agricultural area （UAA） while alternated grassland and grass meadows cover 1.9 million ha. Most of grasslands are located in hilly and mountainous areas and are important for reducing erosion. Italy has a great longitudinal extension which accounts for a great variety of climate systems and soils： the northern regions have a humid subtropical climate and differ greatly from the south part that fits the Mediterranean climate profile. During the last 100/150 years the Italian climate has become warmer and drier showing an increase of erratic precipitation intensity. The future of breeding of forage grasses and legumes should be focused on higher nutrient use efficiencies and increased sustainability. New applications of genomics and bioinformatics will allow advanced breeding strategies. Over the past 15 years breeders have displayed a constant interest in forage species while a greater interest has risen in turfgrass varieties. Seed production of Italian herbages does not cover the requirements of the market. More specific value for cultivation and use （VCU） tests might be an effective means to improve the screening of candidate varieties. The goal is the selection of varieties able to withstand the stress of climate change, have better water and nitrogen use efficiency and resilience of vegetation cover.
