The genetic diversity of 79 accessions regenerated from ＂miniature seeds＂ of loquat （Eriobotrya japonica Lindl. cv. ＇Dawuxing＇） was analyzed based on 13 morphological characteristics of leaves in this study, in ...The genetic diversity of 79 accessions regenerated from ＂miniature seeds＂ of loquat （Eriobotrya japonica Lindl. cv. ＇Dawuxing＇） was analyzed based on 13 morphological characteristics of leaves in this study, in order to provide important morphological basis for breeding new varieties and materials through ＂miniature seed＂ breeding method in loquat. The results showed that there was abundant genetic diversity in leaf morphological characteristics of regenerated plants （D=0.785）, especially indicated by diversity indexes of leaf length （D=0.991）, leaf width （D=0.994）, petiole length （D=0.982）, petiole thickness （D=0.995） and leaf thickness （D=0.987）. And all the characteristic values varied broadly with coefficients of variation ranging from 9.84% to 40.09%; 79 accessions were divided into 6 groups by UPGMA method, there were significant differences in leaf morphological traits among the 6 groups; Group I and group VI were quite different from other groups and parent plants （cv. ＂Dawuxing＂）. The leaves of group I were darkgreen, large, hard and thick with long and thick petioles; while those of group VI were yellowgreen, small, thin and soft with slender petioles. These two groups were important excellent germplasm. Based on the abundant genetic diversity in leaf morphological characteristics, the breeding via ＂miniature seeds＂ was considered as an efficient method for broadening the germplasm resources and breeding new varieties of loquat.展开更多
Fifty two accessions of Festuca pratensis Huds. were analysed of which 18 had been collected in Switzerland and 32 in Bulgaria. SDS-PAGE of seed proteins was implemented to detect genetic variation among ecotype popul...Fifty two accessions of Festuca pratensis Huds. were analysed of which 18 had been collected in Switzerland and 32 in Bulgaria. SDS-PAGE of seed proteins was implemented to detect genetic variation among ecotype populations using a modification of UPOV method for barley. The modification concerns mainly protein extracting procedure as detailed described in the protocol. The two cultivars Preval and Cosmolit were used as standards. Cluster analyses and correspondence analyses/scatter plot were used as statistic approaches for determining genetic diversity among individual ecotypes and groups of ecotypes. Electrophoretic spectra of proteins show clear differences among local accessions in relation to their origin. In Swiss ecotypes 32 protein fragments were determined whereas in Bulgarian local populations their number was 68. Each of the two eco-groups possesses fragments that appear in all accessions of the group. The number of monomorphic bands within Bulgarian local ecotypes is four whereas their number in Swiss ecotypes is 12. Four monomorphic bands appearing in all proteinograms no difference of eco-groups was identified with Rm values of： 0.43, 0.55, 0.58, 0.82. A higher level of protein band polymorphism was proven in Bulgarian ecotypes in comparison with Swiss ecotypes. Thirty seven polymorphic bands occurred exclusively in the Bulgarian local ecotypes and had a frequency of 0.03 or higher whereas within Swiss ecotypes was detected one unique protein fragment. SDS-PAGE ＂fingerprinting＂ is suggested as a fast and easy approach to differentiate F. pratensis ecotypes by their origin as well for detection of foreign germplasm for inclusion in breeding programs.展开更多
文摘The genetic diversity of 79 accessions regenerated from ＂miniature seeds＂ of loquat （Eriobotrya japonica Lindl. cv. ＇Dawuxing＇） was analyzed based on 13 morphological characteristics of leaves in this study, in order to provide important morphological basis for breeding new varieties and materials through ＂miniature seed＂ breeding method in loquat. The results showed that there was abundant genetic diversity in leaf morphological characteristics of regenerated plants （D=0.785）, especially indicated by diversity indexes of leaf length （D=0.991）, leaf width （D=0.994）, petiole length （D=0.982）, petiole thickness （D=0.995） and leaf thickness （D=0.987）. And all the characteristic values varied broadly with coefficients of variation ranging from 9.84% to 40.09%; 79 accessions were divided into 6 groups by UPGMA method, there were significant differences in leaf morphological traits among the 6 groups; Group I and group VI were quite different from other groups and parent plants （cv. ＂Dawuxing＂）. The leaves of group I were darkgreen, large, hard and thick with long and thick petioles; while those of group VI were yellowgreen, small, thin and soft with slender petioles. These two groups were important excellent germplasm. Based on the abundant genetic diversity in leaf morphological characteristics, the breeding via ＂miniature seeds＂ was considered as an efficient method for broadening the germplasm resources and breeding new varieties of loquat.
文摘Fifty two accessions of Festuca pratensis Huds. were analysed of which 18 had been collected in Switzerland and 32 in Bulgaria. SDS-PAGE of seed proteins was implemented to detect genetic variation among ecotype populations using a modification of UPOV method for barley. The modification concerns mainly protein extracting procedure as detailed described in the protocol. The two cultivars Preval and Cosmolit were used as standards. Cluster analyses and correspondence analyses/scatter plot were used as statistic approaches for determining genetic diversity among individual ecotypes and groups of ecotypes. Electrophoretic spectra of proteins show clear differences among local accessions in relation to their origin. In Swiss ecotypes 32 protein fragments were determined whereas in Bulgarian local populations their number was 68. Each of the two eco-groups possesses fragments that appear in all accessions of the group. The number of monomorphic bands within Bulgarian local ecotypes is four whereas their number in Swiss ecotypes is 12. Four monomorphic bands appearing in all proteinograms no difference of eco-groups was identified with Rm values of： 0.43, 0.55, 0.58, 0.82. A higher level of protein band polymorphism was proven in Bulgarian ecotypes in comparison with Swiss ecotypes. Thirty seven polymorphic bands occurred exclusively in the Bulgarian local ecotypes and had a frequency of 0.03 or higher whereas within Swiss ecotypes was detected one unique protein fragment. SDS-PAGE ＂fingerprinting＂ is suggested as a fast and easy approach to differentiate F. pratensis ecotypes by their origin as well for detection of foreign germplasm for inclusion in breeding programs.
