以常规粳稻秀水134为材料,设置清水浸种(CK)和咪酰胺浸种(Soak)两个处理,采用16S r RNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水...以常规粳稻秀水134为材料,设置清水浸种(CK)和咪酰胺浸种(Soak)两个处理,采用16S r RNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水稻种子内细菌群落多样性和组成,包括细菌群落Shannon指数、Chao1指数、Observed OTUs、β多样性以及优势菌群相对丰度没有显著影响。可见,在农业生产中,咪酰胺浸种作为防治水稻种传病害的措施对种子内生菌群没有显著影响。展开更多
The genetic diversity of 79 accessions regenerated from ＂miniature seeds＂ of loquat （Eriobotrya japonica Lindl. cv. ＇Dawuxing＇） was analyzed based on 13 morphological characteristics of leaves in this study, in ...The genetic diversity of 79 accessions regenerated from ＂miniature seeds＂ of loquat （Eriobotrya japonica Lindl. cv. ＇Dawuxing＇） was analyzed based on 13 morphological characteristics of leaves in this study, in order to provide important morphological basis for breeding new varieties and materials through ＂miniature seed＂ breeding method in loquat. The results showed that there was abundant genetic diversity in leaf morphological characteristics of regenerated plants （D=0.785）, especially indicated by diversity indexes of leaf length （D=0.991）, leaf width （D=0.994）, petiole length （D=0.982）, petiole thickness （D=0.995） and leaf thickness （D=0.987）. And all the characteristic values varied broadly with coefficients of variation ranging from 9.84% to 40.09%; 79 accessions were divided into 6 groups by UPGMA method, there were significant differences in leaf morphological traits among the 6 groups; Group I and group VI were quite different from other groups and parent plants （cv. ＂Dawuxing＂）. The leaves of group I were darkgreen, large, hard and thick with long and thick petioles; while those of group VI were yellowgreen, small, thin and soft with slender petioles. These two groups were important excellent germplasm. Based on the abundant genetic diversity in leaf morphological characteristics, the breeding via ＂miniature seeds＂ was considered as an efficient method for broadening the germplasm resources and breeding new varieties of loquat.展开更多
文摘以常规粳稻秀水134为材料,设置清水浸种(CK)和咪酰胺浸种(Soak)两个处理,采用16S r RNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水稻种子内细菌群落多样性和组成,包括细菌群落Shannon指数、Chao1指数、Observed OTUs、β多样性以及优势菌群相对丰度没有显著影响。可见,在农业生产中,咪酰胺浸种作为防治水稻种传病害的措施对种子内生菌群没有显著影响。
文摘The genetic diversity of 79 accessions regenerated from ＂miniature seeds＂ of loquat （Eriobotrya japonica Lindl. cv. ＇Dawuxing＇） was analyzed based on 13 morphological characteristics of leaves in this study, in order to provide important morphological basis for breeding new varieties and materials through ＂miniature seed＂ breeding method in loquat. The results showed that there was abundant genetic diversity in leaf morphological characteristics of regenerated plants （D=0.785）, especially indicated by diversity indexes of leaf length （D=0.991）, leaf width （D=0.994）, petiole length （D=0.982）, petiole thickness （D=0.995） and leaf thickness （D=0.987）. And all the characteristic values varied broadly with coefficients of variation ranging from 9.84% to 40.09%; 79 accessions were divided into 6 groups by UPGMA method, there were significant differences in leaf morphological traits among the 6 groups; Group I and group VI were quite different from other groups and parent plants （cv. ＂Dawuxing＂）. The leaves of group I were darkgreen, large, hard and thick with long and thick petioles; while those of group VI were yellowgreen, small, thin and soft with slender petioles. These two groups were important excellent germplasm. Based on the abundant genetic diversity in leaf morphological characteristics, the breeding via ＂miniature seeds＂ was considered as an efficient method for broadening the germplasm resources and breeding new varieties of loquat.
