Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegr...Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.展开更多
Objective： The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed （EVn-50） on the growth of human cervical carcinoma HeLa cells xenograft...Objective： The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed （EVn-50） on the growth of human cervical carcinoma HeLa cells xenografts in nude mica and its possible molecular mechanism. Methods： Models of human cervical cancer HeLa cells xenografts transplanted subcuta- neously in nude mice were established and randomly divided into 7 groups （each group including 5 nude mice）： saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe- nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels ofLDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blot- ting. Results： EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant effect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion： EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its pro- motion on xenografts cells apoptosis through down-regulation of Bcl-2 expressionPand activation of P53 expression.展开更多
Plant extracts could turn out to be herbicides used in weed control. We evaluated the herbicidal effect of seven plants from the Al-Qassim Region of Saudi Arabia on germination and growth of Phalaris minor, Echinochlo...Plant extracts could turn out to be herbicides used in weed control. We evaluated the herbicidal effect of seven plants from the Al-Qassim Region of Saudi Arabia on germination and growth of Phalaris minor, Echinochloa crusgalli, Portulaca oleracea and Lactuca sativa. In the laboratory, plant extracts at 5, 10, 20 and 40 g/L inhibited the germination and seedling growth of test seeds. P. minor and P. oleracea were more sensitive to the extracts than L. sativa and E. crusgalli. The germination of either P. minor or P. oleracea were completely inhibited by Prosopisjuliflora at 10 g/L and by Rumex vesicarius and Teucrium baccatus at 20 g/L. Extract of Pulicaria undulate completely inhibited germination of target seeds at concentrations ranged from l 0 g/L to 40 g/L. The rank order of bioactivity was Prosopis jul（17ora 〉 Pulicaria undulata 〉 Rumex vesicarius 〉 Withania somnifera 〉 Teucrium baccatus 〉 Artemisia monosperma 〉 Anvillea garcinii. In the greenhouse trial, the extracts of seven plant species at 30, 60 and 90 g/L reduced growth of six-weeks-old target plants. Anvillea garcinii and Prosopisjuliflora showed the greatest effects. At 90 g/L, the reduction in the growth of target species reached between 20% and 30% for Anvillea garcinii and between I 1% and 25% for Prosopis juliflora. Overall, Prosopis juliflora, Pulicaria undulata, Anvillea garcinii and Rumex vesicarius had potential for use as natural herbicides or for development as novel plant-derived herbicides.展开更多
基金Supported by National Natural Science Foundation of China(30471274)~~
文摘Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.
基金Supported by a grant from the Hengyang Municipal Science and Technology Programme(No.2011KJ36)
文摘Objective： The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed （EVn-50） on the growth of human cervical carcinoma HeLa cells xenografts in nude mica and its possible molecular mechanism. Methods： Models of human cervical cancer HeLa cells xenografts transplanted subcuta- neously in nude mice were established and randomly divided into 7 groups （each group including 5 nude mice）： saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe- nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels ofLDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blot- ting. Results： EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant effect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion： EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its pro- motion on xenografts cells apoptosis through down-regulation of Bcl-2 expressionPand activation of P53 expression.
文摘Plant extracts could turn out to be herbicides used in weed control. We evaluated the herbicidal effect of seven plants from the Al-Qassim Region of Saudi Arabia on germination and growth of Phalaris minor, Echinochloa crusgalli, Portulaca oleracea and Lactuca sativa. In the laboratory, plant extracts at 5, 10, 20 and 40 g/L inhibited the germination and seedling growth of test seeds. P. minor and P. oleracea were more sensitive to the extracts than L. sativa and E. crusgalli. The germination of either P. minor or P. oleracea were completely inhibited by Prosopisjuliflora at 10 g/L and by Rumex vesicarius and Teucrium baccatus at 20 g/L. Extract of Pulicaria undulate completely inhibited germination of target seeds at concentrations ranged from l 0 g/L to 40 g/L. The rank order of bioactivity was Prosopis jul（17ora 〉 Pulicaria undulata 〉 Rumex vesicarius 〉 Withania somnifera 〉 Teucrium baccatus 〉 Artemisia monosperma 〉 Anvillea garcinii. In the greenhouse trial, the extracts of seven plant species at 30, 60 and 90 g/L reduced growth of six-weeks-old target plants. Anvillea garcinii and Prosopisjuliflora showed the greatest effects. At 90 g/L, the reduction in the growth of target species reached between 20% and 30% for Anvillea garcinii and between I 1% and 25% for Prosopis juliflora. Overall, Prosopis juliflora, Pulicaria undulata, Anvillea garcinii and Rumex vesicarius had potential for use as natural herbicides or for development as novel plant-derived herbicides.
