The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology （RSM）. Central composite design （CCD） was used to study the interactive effect of culture conditi...The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology （RSM）. Central composite design （CCD） was used to study the interactive effect of culture conditions （temperature, pH, and inoculum） on cellulase activity. Results suggested that temperature and pH all have significant impact on cellulase production. The use of RSM resulted in a 96% increase in the cellulase activity over the control of non-optimized basal medium. Optimum cellulase production of 13 U/mL was obtained at a temperature of 42.24 ℃, pH of 5.25, and inoculum size of 4.95% （v/v） in a fermentation medium containing wheat bran, soybean meal and malt dextrin as major nutritional factors.展开更多
Ten seed borne fungi （Alternaria sp., Aspergillus sp., Aureobasidium sp., Cladosporium sp., Dreschslera sp., Penicillium sp., Rhizoctonia sp., Stemphylium sp., Mueor sp. and Rhizopus sp.） were isolated and identifie...Ten seed borne fungi （Alternaria sp., Aspergillus sp., Aureobasidium sp., Cladosporium sp., Dreschslera sp., Penicillium sp., Rhizoctonia sp., Stemphylium sp., Mueor sp. and Rhizopus sp.） were isolated and identified from two wheat varieties, the highest frequency of seed borne fungi was observed on wheat cultivar site Moll4 Alternaria sp.. Their mean and standard deviation was （5.5 ~ 1.69） while the lowest frequency fungal isolated was Dreschslera sp. and Rhizopus sp.. Their mean and standard deviation was （0.1 ~ 0.64）. The aflatoxin-producing isolates appeared as gray or black colonies in the UV photographs, whereas nonproducing isolates appeared as white colonies, the plate five colony four （P5CO4） showed the positive results which means the presence of aflatoxin as compaired to the control which showed the ngative results. Ammonium Hydroxide Vapor-Induced Color Change method used which the dish was inverted and 1 or 2 drops of concentrated ammonium hydroxide solution are placed on the inside of the lid. The undersides of aflatoxin-producing colonies quickly turn plum-red after the bottom of the Petri dish has been inverted over the lid containing the ammonium hydroxide aspositive result in （P5CO4） and （P7CO4） observed. Essentially no color change occurs on the undersides of colonies that are not producing aflatoxinsthis indicate to the negative results （control）. The main objective of this study is to isolation, identification and rapid detection of aflatoxin from wheat seed borne fungi.展开更多
A pot experiment has been conducted in Faculty of Agricultural and Forestry, Lath House of Horticultural Department, School of Plant Production, Duhok University during the growing season 2010-2011. The study consiste...A pot experiment has been conducted in Faculty of Agricultural and Forestry, Lath House of Horticultural Department, School of Plant Production, Duhok University during the growing season 2010-2011. The study consisted of testing the effects of four different growth media （Clay, Loam, Clay ＋ sheep manure （1：1） and Loam ＋ sheep manure （1：1） as volumetric rates on vegetative and flowering growth characters of five different cultivars of Hyacinths plant （Blue Giant, City of Haarlem, Jon Bos, Delf Blue and Fondante）. The experiment was laid out in randomized complete block design （RCBD） of two factors in three replicates. Media mostly affected the parameters studied during the experiment. Early emergence （22.53 days） and flowering （73.13 days） was recorded in medium consisting of loam and sheep manure, while maximum plant height （19.75 cm）, leaves number （6.13）, leaf area （31.47 cm2） were recorded in clay medium. Maximum number of florets plant-l （31.93） and spike length （7.95 cm） were recorded in medium consist of clay and sheep manure. Maximum length of spike stem （9.61 cm） and total chlorophyll percentage （53.45%） were recorded for loam medium. Among the cultivars, some parameters showed significant variation. Early emergence （20.42 days）, maximum plant height （20.35 cm）, leaf area per plant （33.01 cm2） and length of spike stem （15.10 cm） were given by cv. Fondante. Maximum number of florets plantl （35.58） and spike lengths （8.63 cm） were recorded by cv. Blue Giant. High percentage of total chlorophyll （58.15%） was recorded in cv. Jon Bos..展开更多
文摘The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized using response surface methodology （RSM）. Central composite design （CCD） was used to study the interactive effect of culture conditions （temperature, pH, and inoculum） on cellulase activity. Results suggested that temperature and pH all have significant impact on cellulase production. The use of RSM resulted in a 96% increase in the cellulase activity over the control of non-optimized basal medium. Optimum cellulase production of 13 U/mL was obtained at a temperature of 42.24 ℃, pH of 5.25, and inoculum size of 4.95% （v/v） in a fermentation medium containing wheat bran, soybean meal and malt dextrin as major nutritional factors.
文摘Ten seed borne fungi （Alternaria sp., Aspergillus sp., Aureobasidium sp., Cladosporium sp., Dreschslera sp., Penicillium sp., Rhizoctonia sp., Stemphylium sp., Mueor sp. and Rhizopus sp.） were isolated and identified from two wheat varieties, the highest frequency of seed borne fungi was observed on wheat cultivar site Moll4 Alternaria sp.. Their mean and standard deviation was （5.5 ~ 1.69） while the lowest frequency fungal isolated was Dreschslera sp. and Rhizopus sp.. Their mean and standard deviation was （0.1 ~ 0.64）. The aflatoxin-producing isolates appeared as gray or black colonies in the UV photographs, whereas nonproducing isolates appeared as white colonies, the plate five colony four （P5CO4） showed the positive results which means the presence of aflatoxin as compaired to the control which showed the ngative results. Ammonium Hydroxide Vapor-Induced Color Change method used which the dish was inverted and 1 or 2 drops of concentrated ammonium hydroxide solution are placed on the inside of the lid. The undersides of aflatoxin-producing colonies quickly turn plum-red after the bottom of the Petri dish has been inverted over the lid containing the ammonium hydroxide aspositive result in （P5CO4） and （P7CO4） observed. Essentially no color change occurs on the undersides of colonies that are not producing aflatoxinsthis indicate to the negative results （control）. The main objective of this study is to isolation, identification and rapid detection of aflatoxin from wheat seed borne fungi.
文摘A pot experiment has been conducted in Faculty of Agricultural and Forestry, Lath House of Horticultural Department, School of Plant Production, Duhok University during the growing season 2010-2011. The study consisted of testing the effects of four different growth media （Clay, Loam, Clay ＋ sheep manure （1：1） and Loam ＋ sheep manure （1：1） as volumetric rates on vegetative and flowering growth characters of five different cultivars of Hyacinths plant （Blue Giant, City of Haarlem, Jon Bos, Delf Blue and Fondante）. The experiment was laid out in randomized complete block design （RCBD） of two factors in three replicates. Media mostly affected the parameters studied during the experiment. Early emergence （22.53 days） and flowering （73.13 days） was recorded in medium consisting of loam and sheep manure, while maximum plant height （19.75 cm）, leaves number （6.13）, leaf area （31.47 cm2） were recorded in clay medium. Maximum number of florets plant-l （31.93） and spike length （7.95 cm） were recorded in medium consist of clay and sheep manure. Maximum length of spike stem （9.61 cm） and total chlorophyll percentage （53.45%） were recorded for loam medium. Among the cultivars, some parameters showed significant variation. Early emergence （20.42 days）, maximum plant height （20.35 cm）, leaf area per plant （33.01 cm2） and length of spike stem （15.10 cm） were given by cv. Fondante. Maximum number of florets plantl （35.58） and spike lengths （8.63 cm） were recorded by cv. Blue Giant. High percentage of total chlorophyll （58.15%） was recorded in cv. Jon Bos..
