Bhutanese maize farmers grow different open-pollinated traditional varieties and improved high yielding varieties recommended by the national maize program. All most every maize farmer uses the recycled seeds from the...Bhutanese maize farmers grow different open-pollinated traditional varieties and improved high yielding varieties recommended by the national maize program. All most every maize farmer uses the recycled seeds from their farms for planting in the next season. Farmers traditional or informal seed system was found to be poorly organized and unscientific that has resulted in the deterioration and contamination of seed quality. Bhutanese maize farmers living in remote areas are highly constrained by the inconsistent supply and poor access to good quality maize seed. The formal public seed sectors lack adequate resources to produce and supply good quality seeds. This called for an urgency to identify and adapt an innovative and a sustainable seed production approach to service the subsistence needs of Bhutanese farmers in a cross-pollinated crop, like maize. The community based seed production (CBSP) approach was introduced and evaluated under the Bhutanese maize production system as an alternative farmer based seed production model. A total of seven farmers CBSP groups were formed and promoted as a smallholder seed enterprise. The technical skills of these CBSP group members on maize seed production were improved through practical demonstrations and trainings. The CBSP groups were supplied with high quality source seed of new maize varieties by the national maize program. For long term, the sustainability of CBSP groups were linked to the formal seed sector in the country for marketing the seeds. The CBSP approach has proven to be a successful small scale maize seed production model under highland maize production ecosystem that has improved the production and supply of good quality maize seeds. The Bhutanese experiences with CBSP in maize, its merits for small holder Bhutanese maize farmers and lessons learnt from the CBSP model are discussed in this paper.展开更多
Coptis chinensis is a medicinal herb used in traditional Chinese medicine.This research features a case study in Shizhu County,China.Participatory Rural Appraisal(PRA) is used as the main tool for data collection.The ...Coptis chinensis is a medicinal herb used in traditional Chinese medicine.This research features a case study in Shizhu County,China.Participatory Rural Appraisal(PRA) is used as the main tool for data collection.The study demonstrates that the current method of Coptis planting leads to forest destruction.Even with reforestation measures,it leads to the loss of biodiversity in the forest.It also shows that farmers cannot get satisfactory economic returns from Coptis planting,as the return is unstable and unpredictable.One alternative to the current Coptis planting mode is to incorporate it into the agroforestry system.Water shield planting also serves as a good alternative to Coptis planting itself.Improved methods of management,post-harvest processing,and marketing of Coptis are also proposed and analyzed.The intended contribution of this paper to the theoretical debate of sustainability shall be the provision of an example showing how agricultural production affects forest conservation.It also presents redressable measures to the negative consequences.Besides,one alternative livelihood pathway for the rural community in this case study is suggested.展开更多
A pot experiment was performed to determine the effects of arbuscular mycorrhizM fungi (AMF) communities on soil properties and the growth of cucumber seedlings in a degraded soil that had been used for continuous c...A pot experiment was performed to determine the effects of arbuscular mycorrhizM fungi (AMF) communities on soil properties and the growth of cucumber seedlings in a degraded soil that had been used for continuous cucumber monoculture in a greenhouse for 15 years. In the experiment, AMF communities (created by combining various AMF species that were found to be dominant in natural farm soil) were inoculated into the degraded soil, and then the soil was planted with cucumber. Inoculation with AMF communities did not affect soil pH but increased soil aggregate stability and decreased the concentrations of salt ions and electrical conductivity (EC) in the soil. Inoculation with AMF communities increased the numbers of culturable bacteria and actinomycetes but reduced the number of fungi. AMF communities increased plant growth, soluble sugar content, chlorophyll content, and root activity compared to non-mycorrhizal or a single AMF species treatments. Improvements of soil quality and plant growth were greatest with the following two communities: Glomus etunicatum + G. mosseae + Gigaspora margarita + Acaulospora lacunosa and G. aggregatum + G. etunicatum + G. mosseae + G. versiforme + G. margarita + A. lacunosa. The results suggested that certain AMF communities could substantially improve the quality of degraded soil.展开更多
There is an increasing concern that the continuous use of chemical fertilizers might lead to harmful effects on soil ecosystem.Accordingly, a biocompatible approach involving inoculation of beneficial microorganisms i...There is an increasing concern that the continuous use of chemical fertilizers might lead to harmful effects on soil ecosystem.Accordingly, a biocompatible approach involving inoculation of beneficial microorganisms is presented to promote plant growth and simultaneously minimize the negative effect of chemical fertilizers. In this study, Rhodopseudomonas palustris, a plant growth-promoting rhizobacterium(PGPR), was inoculated into both fertilized and unfertilized soils to assess its influence on Stevia rebaudiana plant growth and microbial community in rhizosphere soils in a 122-d field experiment. Soil enzyme assays(dehydrogenase, urease, invertase, and phosphomonoesterase), real-time quantitative polymerase chain reaction(RT-_qPCR), and a high-throughput sequencing technique were employed to determine the microbial activity and characterize the bacterial community. Results showed that the R.palustris inoculation did not significantly influence Stevia yields and root biomass in either the fertilized or unfertilized soil. Chemical fertilization had strong negative effects on soil bacterial community properties, especially on dehydrogenase and urease activities.However, R. palustris inoculation counteracted the effect of chemical fertilizer on dehydrogenase and urease activities, and increased the abundances of some bacterial lineages(including Bacteroidia, Nitrospirae, Planctomycetacia, Myxococcales, and Legionellales). In contrast, inoculation into the unfertilized soil did not significantly change the soil enzyme activities or the soil bacterial community structure. For both the fertilized and unfertilized soils, R. palustris inoculation decreased the relative abundances of some bacterial lineages possessing photosynthetic ability, such as Cyanobacteria, Rhodobacter, Sphingomonadales, and Burkholderiales. Taken together, our observations stress the potential utilization of R. palustris as PGPR in agriculture, which might further ameliorate the soil microbial properties in the long run.展开更多
文摘Bhutanese maize farmers grow different open-pollinated traditional varieties and improved high yielding varieties recommended by the national maize program. All most every maize farmer uses the recycled seeds from their farms for planting in the next season. Farmers traditional or informal seed system was found to be poorly organized and unscientific that has resulted in the deterioration and contamination of seed quality. Bhutanese maize farmers living in remote areas are highly constrained by the inconsistent supply and poor access to good quality maize seed. The formal public seed sectors lack adequate resources to produce and supply good quality seeds. This called for an urgency to identify and adapt an innovative and a sustainable seed production approach to service the subsistence needs of Bhutanese farmers in a cross-pollinated crop, like maize. The community based seed production (CBSP) approach was introduced and evaluated under the Bhutanese maize production system as an alternative farmer based seed production model. A total of seven farmers CBSP groups were formed and promoted as a smallholder seed enterprise. The technical skills of these CBSP group members on maize seed production were improved through practical demonstrations and trainings. The CBSP groups were supplied with high quality source seed of new maize varieties by the national maize program. For long term, the sustainability of CBSP groups were linked to the formal seed sector in the country for marketing the seeds. The CBSP approach has proven to be a successful small scale maize seed production model under highland maize production ecosystem that has improved the production and supply of good quality maize seeds. The Bhutanese experiences with CBSP in maize, its merits for small holder Bhutanese maize farmers and lessons learnt from the CBSP model are discussed in this paper.
基金supported by EU-China Biodiversity Programme Chongqing Office
文摘Coptis chinensis is a medicinal herb used in traditional Chinese medicine.This research features a case study in Shizhu County,China.Participatory Rural Appraisal(PRA) is used as the main tool for data collection.The study demonstrates that the current method of Coptis planting leads to forest destruction.Even with reforestation measures,it leads to the loss of biodiversity in the forest.It also shows that farmers cannot get satisfactory economic returns from Coptis planting,as the return is unstable and unpredictable.One alternative to the current Coptis planting mode is to incorporate it into the agroforestry system.Water shield planting also serves as a good alternative to Coptis planting itself.Improved methods of management,post-harvest processing,and marketing of Coptis are also proposed and analyzed.The intended contribution of this paper to the theoretical debate of sustainability shall be the provision of an example showing how agricultural production affects forest conservation.It also presents redressable measures to the negative consequences.Besides,one alternative livelihood pathway for the rural community in this case study is suggested.
基金Supported by the National Natural Science Foundation of China (No. 30871737)the 2010 Open Foundation of State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences(No. Y052010038)
文摘A pot experiment was performed to determine the effects of arbuscular mycorrhizM fungi (AMF) communities on soil properties and the growth of cucumber seedlings in a degraded soil that had been used for continuous cucumber monoculture in a greenhouse for 15 years. In the experiment, AMF communities (created by combining various AMF species that were found to be dominant in natural farm soil) were inoculated into the degraded soil, and then the soil was planted with cucumber. Inoculation with AMF communities did not affect soil pH but increased soil aggregate stability and decreased the concentrations of salt ions and electrical conductivity (EC) in the soil. Inoculation with AMF communities increased the numbers of culturable bacteria and actinomycetes but reduced the number of fungi. AMF communities increased plant growth, soluble sugar content, chlorophyll content, and root activity compared to non-mycorrhizal or a single AMF species treatments. Improvements of soil quality and plant growth were greatest with the following two communities: Glomus etunicatum + G. mosseae + Gigaspora margarita + Acaulospora lacunosa and G. aggregatum + G. etunicatum + G. mosseae + G. versiforme + G. margarita + A. lacunosa. The results suggested that certain AMF communities could substantially improve the quality of degraded soil.
基金supported by the National Natural Science Foundation of China (No. 41501264)the Natural Science Foundation of Jiangsu Province, China (No. BK20140991)the Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Nanjing Institute of Soil Science, Chinese Academy of Sciences (No. Y412201441)
文摘There is an increasing concern that the continuous use of chemical fertilizers might lead to harmful effects on soil ecosystem.Accordingly, a biocompatible approach involving inoculation of beneficial microorganisms is presented to promote plant growth and simultaneously minimize the negative effect of chemical fertilizers. In this study, Rhodopseudomonas palustris, a plant growth-promoting rhizobacterium(PGPR), was inoculated into both fertilized and unfertilized soils to assess its influence on Stevia rebaudiana plant growth and microbial community in rhizosphere soils in a 122-d field experiment. Soil enzyme assays(dehydrogenase, urease, invertase, and phosphomonoesterase), real-time quantitative polymerase chain reaction(RT-_qPCR), and a high-throughput sequencing technique were employed to determine the microbial activity and characterize the bacterial community. Results showed that the R.palustris inoculation did not significantly influence Stevia yields and root biomass in either the fertilized or unfertilized soil. Chemical fertilization had strong negative effects on soil bacterial community properties, especially on dehydrogenase and urease activities.However, R. palustris inoculation counteracted the effect of chemical fertilizer on dehydrogenase and urease activities, and increased the abundances of some bacterial lineages(including Bacteroidia, Nitrospirae, Planctomycetacia, Myxococcales, and Legionellales). In contrast, inoculation into the unfertilized soil did not significantly change the soil enzyme activities or the soil bacterial community structure. For both the fertilized and unfertilized soils, R. palustris inoculation decreased the relative abundances of some bacterial lineages possessing photosynthetic ability, such as Cyanobacteria, Rhodobacter, Sphingomonadales, and Burkholderiales. Taken together, our observations stress the potential utilization of R. palustris as PGPR in agriculture, which might further ameliorate the soil microbial properties in the long run.