This study aimed to screen drought stress indicators and analyze the drought resistance in 105 cultivated rice germplasm resources in Guangxi Zhuang Autonomous Region under drought stress in the whole growth period ba...This study aimed to screen drought stress indicators and analyze the drought resistance in 105 cultivated rice germplasm resources in Guangxi Zhuang Autonomous Region under drought stress in the whole growth period based on D value of seven morphological traits including panicle neck thickness,effective panicle number per plant,1 000-grain weight,filled grain number per panicle,total grain number,seed setting rate and yield per plant.According to the results,under drought stress,yield per plant exhibited significant positive correlation with panicle neck thickness and exhibited extremely significant positive correlation with other five morphological traits.Based on the identification and evaluation of comprehensive drought-resistance index D value,seven highly drought-resistant cultivated rice varieties were screened,accounting for 6.67％.Upland rice germplasm resources in Guangxi Zhuang Autonomous Region possess strong drought-resistance capacity,which could be used to screen highly drought-resistant rice germplasm materials.This study provided material basis and theoretical reference for breeding drought resistant rice cultivars in Guangxi Zhuang Autonomous Region.展开更多
Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank ...Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province.展开更多
For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination ind...For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination index and salt-injury rate, 128 peanut germplasms were selected for salt-tolerant identification and estimation under 2.5% NaCI. We found significant but various depressing levels of germination rate under salt stress among different germplasms, and only 5% of 128 germplasms were highly resistant to salinity. We also found that the relative germination index was a useful evaluation index for salt tolerance besides salt damage rate and rela- tive germination rate. After all, we comprehensively screened out 7 materials （JS011, JS024, JS125, JS491, JS523, JS524 and JS525） as salt tolerance germplasms for further research. Key展开更多
[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European sa...[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.展开更多
Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains...Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA （RAPD）. A single 10-based primer was used to generate randomly amplified polymorphic DNA （RAPD） in V. volvacea and differences were noted in band size （bp） ranging from 1,800 bp to 550 bp. Principal component analysis （PCA） of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.展开更多
Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic...Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic research which is good for developing and expanding tea well-bred varieties in tea field, especially in quality selection and biotechnology research. Furthermore, excellent tea germplasm is primary condition for tea breeding and quality. Therefore, identification and screening excellent tea germplasm become particularly important. We need accelerate develop high-quality tea varieties, and it need to base on exploring and studying tea germplasm. This paper reviews the research progress of tea germplasm on identification from morphological characteristic, biological characteristic, quality characteristic, resistance and heredity. However, there are still some problems on identification and screening of tea germplasm, no effective methods have been found so far which apply on early identification and genetic engineering, so we should make intensive and long lasting study tea germplasm and analyze systematically various identification, which are benefit for breeding high-quality seeds, shortening the breeding period and summing the new way of breeding. We expect them to play the key roles and provide the theory basis for genetic improvement, classification and breeding in tea plant.展开更多
基金Supported by Special Fund for Bagui Scholars of Guangxi Zhuang Autonomous RegionNational Science and Technology Support Program of China (2012BAD40B04,2013BAD01B02-17)+3 种基金Project for Scientific Research and Technological Development of Guangxi Zhuang Autonomous Region (GKG1123001-3C)Youth Fund of Guangxi Zhuang Autonomous Region(2013GXNSFBA019052,2013GXNSFAA019054)Fund for Science and Technology Development of Guangxi Academy of Agricultural Sciences(GNK2014JQ13)Special Fund for Basic Scientific Research of Guangxi Academy of Agricultural Sciences(2013YT05)~~
文摘This study aimed to screen drought stress indicators and analyze the drought resistance in 105 cultivated rice germplasm resources in Guangxi Zhuang Autonomous Region under drought stress in the whole growth period based on D value of seven morphological traits including panicle neck thickness,effective panicle number per plant,1 000-grain weight,filled grain number per panicle,total grain number,seed setting rate and yield per plant.According to the results,under drought stress,yield per plant exhibited significant positive correlation with panicle neck thickness and exhibited extremely significant positive correlation with other five morphological traits.Based on the identification and evaluation of comprehensive drought-resistance index D value,seven highly drought-resistant cultivated rice varieties were screened,accounting for 6.67％.Upland rice germplasm resources in Guangxi Zhuang Autonomous Region possess strong drought-resistance capacity,which could be used to screen highly drought-resistant rice germplasm materials.This study provided material basis and theoretical reference for breeding drought resistant rice cultivars in Guangxi Zhuang Autonomous Region.
文摘Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province.
基金Supported by National Peanut Industry Technology System"Nanjing Comprehensive Experimental Station"(CARS-14)the Fund of Independent Innovation of Agricultural Science and Technology in Jiangsu Province"On the Collection,Identification and Shared Service of Germplasm Resources of Main Field Crops"[CX(14)2001]~~
文摘For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination index and salt-injury rate, 128 peanut germplasms were selected for salt-tolerant identification and estimation under 2.5% NaCI. We found significant but various depressing levels of germination rate under salt stress among different germplasms, and only 5% of 128 germplasms were highly resistant to salinity. We also found that the relative germination index was a useful evaluation index for salt tolerance besides salt damage rate and rela- tive germination rate. After all, we comprehensively screened out 7 materials （JS011, JS024, JS125, JS491, JS523, JS524 and JS525） as salt tolerance germplasms for further research. Key
基金Supported by National Peach Industrial Technology System (nycytx-31-zs-10 )National Science and Technology Support Program (2008BAD98B03-08)+1 种基金National Peach Commonweal Science (Agriculture) Research Projects (3-37)Chengdu Technology Application and Promotion Program (09YTZD986NC-012)
文摘[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.
文摘Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA （RAPD）. A single 10-based primer was used to generate randomly amplified polymorphic DNA （RAPD） in V. volvacea and differences were noted in band size （bp） ranging from 1,800 bp to 550 bp. Principal component analysis （PCA） of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.
文摘Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic research which is good for developing and expanding tea well-bred varieties in tea field, especially in quality selection and biotechnology research. Furthermore, excellent tea germplasm is primary condition for tea breeding and quality. Therefore, identification and screening excellent tea germplasm become particularly important. We need accelerate develop high-quality tea varieties, and it need to base on exploring and studying tea germplasm. This paper reviews the research progress of tea germplasm on identification from morphological characteristic, biological characteristic, quality characteristic, resistance and heredity. However, there are still some problems on identification and screening of tea germplasm, no effective methods have been found so far which apply on early identification and genetic engineering, so we should make intensive and long lasting study tea germplasm and analyze systematically various identification, which are benefit for breeding high-quality seeds, shortening the breeding period and summing the new way of breeding. We expect them to play the key roles and provide the theory basis for genetic improvement, classification and breeding in tea plant.
