RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. olig...RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. oligospermatangia . Among the total 69 fragments generated by 6 selected primers (among 50 primers), 67 appeared to be polymorphic (97.1%). Cluster analysis based on the RAPD results was performed. The 15 Porphyra lines were divided into 3 groups. This result was consistent with that from taxonomy analysis. A DNA fingerprinting based on 8 bands amplified with OPN_02 and OPJ_18 was constructed and might be used in Porphyra variety identification. Five specific RAPD fragments of 5 Porphyra lines were isolated and cloned into pGEM_T easy vector. These five RAPD fragments may be useful in germplasm identification and property protection of Porphyra .展开更多
[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers se...[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.展开更多
The development of genetics has entered into new genetic markers application ysage which developed one after the other from Mendelian morphological marker, cytogenetics marker, allozyme marker to DNA markers in algal ...The development of genetics has entered into new genetic markers application ysage which developed one after the other from Mendelian morphological marker, cytogenetics marker, allozyme marker to DNA markers in algal research.Based on morphological features such as the colours and seed sizes of pea, Mendel discovered the famous genetic laws. The cytogenetics markers included chromosome morphology, number and structure, which can be used for gene localization. Allozymes are allelic variants of proteins produced by a single gene locus, and are of interest as markers because of the existing polymorphism. Since the 1970s, it has been used widespread for population genetics and other fields in biology. Since the last decade of the 20th century, various DNA marker techniques have been invented, such as RFLP, RAPD, AFLP, SSR and SNP. With DNA markers, it is conveniently and easily to observe and exploit genetic variation in the entire genome. However, the application of molecular markers is still in its primary level. Here the principles and characters of kinds of molecular markers were reviewed including their applications in algae research, respectively.展开更多
文摘RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. oligospermatangia . Among the total 69 fragments generated by 6 selected primers (among 50 primers), 67 appeared to be polymorphic (97.1%). Cluster analysis based on the RAPD results was performed. The 15 Porphyra lines were divided into 3 groups. This result was consistent with that from taxonomy analysis. A DNA fingerprinting based on 8 bands amplified with OPN_02 and OPJ_18 was constructed and might be used in Porphyra variety identification. Five specific RAPD fragments of 5 Porphyra lines were isolated and cloned into pGEM_T easy vector. These five RAPD fragments may be useful in germplasm identification and property protection of Porphyra .
基金Supported by Hunan Provincial Natural Science Foundation of China(2008JJ3035)~~
文摘[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.
文摘The development of genetics has entered into new genetic markers application ysage which developed one after the other from Mendelian morphological marker, cytogenetics marker, allozyme marker to DNA markers in algal research.Based on morphological features such as the colours and seed sizes of pea, Mendel discovered the famous genetic laws. The cytogenetics markers included chromosome morphology, number and structure, which can be used for gene localization. Allozymes are allelic variants of proteins produced by a single gene locus, and are of interest as markers because of the existing polymorphism. Since the 1970s, it has been used widespread for population genetics and other fields in biology. Since the last decade of the 20th century, various DNA marker techniques have been invented, such as RFLP, RAPD, AFLP, SSR and SNP. With DNA markers, it is conveniently and easily to observe and exploit genetic variation in the entire genome. However, the application of molecular markers is still in its primary level. Here the principles and characters of kinds of molecular markers were reviewed including their applications in algae research, respectively.
