[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek ...[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.展开更多
Through 5 years of phenological observations on Larix principis-rupprechtii Mayr. in primary seed orchard and studies on population and individuals of clones, the annual periodic phenological laws were revealed and th...Through 5 years of phenological observations on Larix principis-rupprechtii Mayr. in primary seed orchard and studies on population and individuals of clones, the annual periodic phenological laws were revealed and the annual phe-nological periodic table was drawn up. The correlation between various phenophases, the air temperature and active accumu-lated temperature were analyzed and expounded. The authors also analyzed the similarities and differences of phenophases among clonal individuals as well as the blooming properties of male and female flowers at the same time. This study could pro-vide theoretical reference for working out the production plan of improved varieties and other management measures in seed orchard of Larix principis-rupprechtii.展开更多
Using the commercial seeds of two hybrid rice varieties including Lu- liangyou 996 and Liangyoupeijiu as the materials, four specific gravity-based seed grading treatments, Le., the specific gravity of 〈1.0 （T1）, 1...Using the commercial seeds of two hybrid rice varieties including Lu- liangyou 996 and Liangyoupeijiu as the materials, four specific gravity-based seed grading treatments, Le., the specific gravity of 〈1.0 （T1）, 1.0-1.09 （T2）, 1.1-1.19（T3） and ≥1.2 （T4）, by selection with different saline solutions, and the control without seed grading （CK） were designed to study the effects of seed grading on seed germination, seedling emergence, seedling quality and grain yield. The results showed that the treatments of T2, T3 and T4 had higher or significantly higher seed germination rate, germination index and vigor index, seedling emergence rate and adult seedling rate than the CK, while T1 had significantly lower values of these traits than the CK. Compared with the CK, the number of spikelets per pani- cle was found to be the main reason for the yield increase of these treatments with high seed viability.展开更多
In order to investigate the effects of seed rate on root twining power and seedling quality of machine-transplanted super rice cultivars, Yongyou 12 and Zhongzheyou No.l, total 6 seed rates were arranged, and the grow...In order to investigate the effects of seed rate on root twining power and seedling quality of machine-transplanted super rice cultivars, Yongyou 12 and Zhongzheyou No.l, total 6 seed rates were arranged, and the growth characteris- tics, quality and root twining power of machine-transplanted rice seedlings were studied. The results showed that seed rate had great effects on root twining power and qualityof rice seedlings; with the increase of seed rate, the root twining power of rice seedlings was increased, but the quality of rice seedlings was significantly reduced; under the condition of high seed rate, rice seedlings were weak with less accumulate dry matter and nonuniform growth, and the proportion of weak seedlings was higher. When the seed rate of both rice cultivars was reduced by 10 g/tray, the average seedling height was increased by 0.5 cm, leaf age was increased by 0.1, stem base diameter was increased by 0.1 cm, and root number per plant was increased by 0.3. When the seed rate was increased by 10 g/tray, the accumulated dry matter per plant was reduced by 5.1 mg. With the increase of seed rate, the seedling quality of Zhongzheyou No.1 was decreased rapidly, and the proportion of weak seedlings was increased rapidly. However, Yongyou 12 has a larger elasticity in seed rate. When the seed rate reached to 50 g/tray, blanket seedlings of Yongy- ou 12 were nursed with uniform growth and higher quality, but the shoots were sparse with lower leaf area index. However, when the seed rate was up to 50 g/tray, blanket seedlings of Zhongzheyou No.1 were nursed with uniform growth and higher quality, as well as higher-density shoots. From a comprehensive point of view, the optimum seed rates of Yongyou 12 and Zhongzheyou No.1 were 65 and 50 g/tray, respectively.展开更多
The research conducted investigation and survey on 186 farm households in 2014 and carried out economic evaluation on ＂planting three to produce four＂ highyielding project of super hybrid rice. The results showed th...The research conducted investigation and survey on 186 farm households in 2014 and carried out economic evaluation on ＂planting three to produce four＂ highyielding project of super hybrid rice. The results showed that the project of ＂planting three to produce four＂ performed significantly in terms of increasing yields and incomes. For example, per unit area yield of one-season late hybrid rice and middleseason hybrid rice proved the highest, and net income from middle-season rice in Longhui was the highest. It is obvious that to increase per unit yield is key for improving net incomes and to reduce machine fees and worker fees is crucial for decreasing fees.展开更多
Allelopathy was defined in 1996 by IAS （international allelopathy society） as any process involving secondary metabolites produced by plant, algae, bacteria and fungi that influences the growth and development of ag...Allelopathy was defined in 1996 by IAS （international allelopathy society） as any process involving secondary metabolites produced by plant, algae, bacteria and fungi that influences the growth and development of agricultural and biological systems. Rice allelopathy against weeds was reported since 1989, which offers an integrated weed management with substantially reduced herbicide usage. Application of allelopathic rice cultivars is thought a resources conservation and environmental friendly way of weed bio-control, and could promote the sustainable development of agriculture. Screening or evaluating the allelopathic potential rice variety is the first step. In this paper, a new bioassay method was set up by the allelopathic potential of 9 rice lines on the target weed barnyardgrass （Echinochloa crusgalli）, and comparing with bioassay methods such as relay seeding in filter paper （RSF） and relay seeding in agar （RSA）. The results indicated that three methods had a same tendency in evaluating the allelopathic potential of rice; there existed a significant difference among different bioassay methods, and an interaction between bioassay methods and rice lines. The method of root exudates （RE） with the highest value and a correlation efficiency of 0.98 was considered as ideal bioassay method for evaluation of allelopathic potential.展开更多
基金Supported by Natural Science Foundation of Hebei Province(C2006000147)Zhengzhou Science and Technology Program(10PTGN449-6)~~
文摘[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.
文摘Through 5 years of phenological observations on Larix principis-rupprechtii Mayr. in primary seed orchard and studies on population and individuals of clones, the annual periodic phenological laws were revealed and the annual phe-nological periodic table was drawn up. The correlation between various phenophases, the air temperature and active accumu-lated temperature were analyzed and expounded. The authors also analyzed the similarities and differences of phenophases among clonal individuals as well as the blooming properties of male and female flowers at the same time. This study could pro-vide theoretical reference for working out the production plan of improved varieties and other management measures in seed orchard of Larix principis-rupprechtii.
基金Supported by Special Scientific Research Fund of Agricultural Public Welfare Profession of China(201303002,201203052)Key Project of Education Department of Hunan Province(14A073)~~
文摘Using the commercial seeds of two hybrid rice varieties including Lu- liangyou 996 and Liangyoupeijiu as the materials, four specific gravity-based seed grading treatments, Le., the specific gravity of 〈1.0 （T1）, 1.0-1.09 （T2）, 1.1-1.19（T3） and ≥1.2 （T4）, by selection with different saline solutions, and the control without seed grading （CK） were designed to study the effects of seed grading on seed germination, seedling emergence, seedling quality and grain yield. The results showed that the treatments of T2, T3 and T4 had higher or significantly higher seed germination rate, germination index and vigor index, seedling emergence rate and adult seedling rate than the CK, while T1 had significantly lower values of these traits than the CK. Compared with the CK, the number of spikelets per pani- cle was found to be the main reason for the yield increase of these treatments with high seed viability.
基金Supported by National Rice Industry Technology System(CARS-01-04A)National Key Technology Research and Development Program(2012BAD07B02)+1 种基金Ministry of Science and Technology’s Special Fund for Technology Development and Research of Research Institutes(2013EG134237)National Nonprofit Institute Research Grant of CATAS-TCGRI(2012RG005-3)~~
文摘In order to investigate the effects of seed rate on root twining power and seedling quality of machine-transplanted super rice cultivars, Yongyou 12 and Zhongzheyou No.l, total 6 seed rates were arranged, and the growth characteris- tics, quality and root twining power of machine-transplanted rice seedlings were studied. The results showed that seed rate had great effects on root twining power and qualityof rice seedlings; with the increase of seed rate, the root twining power of rice seedlings was increased, but the quality of rice seedlings was significantly reduced; under the condition of high seed rate, rice seedlings were weak with less accumulate dry matter and nonuniform growth, and the proportion of weak seedlings was higher. When the seed rate of both rice cultivars was reduced by 10 g/tray, the average seedling height was increased by 0.5 cm, leaf age was increased by 0.1, stem base diameter was increased by 0.1 cm, and root number per plant was increased by 0.3. When the seed rate was increased by 10 g/tray, the accumulated dry matter per plant was reduced by 5.1 mg. With the increase of seed rate, the seedling quality of Zhongzheyou No.1 was decreased rapidly, and the proportion of weak seedlings was increased rapidly. However, Yongyou 12 has a larger elasticity in seed rate. When the seed rate reached to 50 g/tray, blanket seedlings of Yongy- ou 12 were nursed with uniform growth and higher quality, but the shoots were sparse with lower leaf area index. However, when the seed rate was up to 50 g/tray, blanket seedlings of Zhongzheyou No.1 were nursed with uniform growth and higher quality, as well as higher-density shoots. From a comprehensive point of view, the optimum seed rates of Yongyou 12 and Zhongzheyou No.1 were 65 and 50 g/tray, respectively.
文摘The research conducted investigation and survey on 186 farm households in 2014 and carried out economic evaluation on ＂planting three to produce four＂ highyielding project of super hybrid rice. The results showed that the project of ＂planting three to produce four＂ performed significantly in terms of increasing yields and incomes. For example, per unit area yield of one-season late hybrid rice and middleseason hybrid rice proved the highest, and net income from middle-season rice in Longhui was the highest. It is obvious that to increase per unit yield is key for improving net incomes and to reduce machine fees and worker fees is crucial for decreasing fees.
文摘Allelopathy was defined in 1996 by IAS （international allelopathy society） as any process involving secondary metabolites produced by plant, algae, bacteria and fungi that influences the growth and development of agricultural and biological systems. Rice allelopathy against weeds was reported since 1989, which offers an integrated weed management with substantially reduced herbicide usage. Application of allelopathic rice cultivars is thought a resources conservation and environmental friendly way of weed bio-control, and could promote the sustainable development of agriculture. Screening or evaluating the allelopathic potential rice variety is the first step. In this paper, a new bioassay method was set up by the allelopathic potential of 9 rice lines on the target weed barnyardgrass （Echinochloa crusgalli）, and comparing with bioassay methods such as relay seeding in filter paper （RSF） and relay seeding in agar （RSA）. The results indicated that three methods had a same tendency in evaluating the allelopathic potential of rice; there existed a significant difference among different bioassay methods, and an interaction between bioassay methods and rice lines. The method of root exudates （RE） with the highest value and a correlation efficiency of 0.98 was considered as ideal bioassay method for evaluation of allelopathic potential.
