ISSR Analysis of Genetic Relationship of Germplasm Resource in Prunus mume Sibe. et Zucc

Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.

TBP分子标记技术的原理及应用研究进展

基于微管蛋白多态性(tubulin-based polymorphism,简称TBP)是一种新型的分子标记技术,通过对植物β微管蛋白基因编码区的第1个内含子来进行遗传多样性分析,目前已被广泛应用于研究多种植物。概述TBP标记的原理、特点、引物设计方法、PCR反应体系及其产物检测方法,并介绍其在种质资源遗传多样性与亲缘关系分析等方面应用进展,同时,对其存在的问题进行讨论,并展望该分子标记的应用前景。