In July 2006, the diurnal activity rhythms of 13 heads of captive Qinling golden takin were observed in Shaanxi Provincial Centre for the Breeding and Conservation of Rare Wildlife (SPCBCRW) at daylight (from 9:00...In July 2006, the diurnal activity rhythms of 13 heads of captive Qinling golden takin were observed in Shaanxi Provincial Centre for the Breeding and Conservation of Rare Wildlife (SPCBCRW) at daylight (from 9:00 am to 4:00 pm in July). The behavioral ethnogram was identified through observation at the sampling site, with the behaviors of golden takin recorded at 5 min interval using instantaneous scannable sampling method. The results indicated that the resting of golden takin took an important part in the daylight activities. Meanwhile, drinking and urinating and defecating were 1.92 and 0.54 and 0.92 times per head per day. Ambient temperature had a strong effect on the diurnal activity rhythms of golden takin. The diurnal activity rhymes were affected by ages of the animals effectively, which was expressed through differentiation of the time budgets. Moreover, different individuals in the same population showed some non-synchronously activity rhythms.展开更多
[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evalua...[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evaluation.[Method] The cpDNA psbA-trnH and nrDNA ITS sequences of five Chinese herb Qingjiao plants,including Gentiana macrophylla pall.,Gentiana straminea Maxim.,Gentiana crassicaulis Duthie ex Burk.,Gentiana dahurica Fisch and Gentiana officinalis H.Smith,were amplified with PCR,and then sequenced by direct PCR sequencing method for homologous analysis.[Results] The length of cpDNA psbA-trnH of five plants was 316-318 bp;there were seven different haplotypes and seven variable sites;the GC content of the sequence was 21.2%;the phylogenetic clustering showed the same result as haplotype analysis.The length of nrDNA ITS sequence of five plants was 624-625 bp,there were five different haplotypes and 13 variable sites;the GC content of the sequence was 59.3%.The result of phylogenetic clustering suggested that G.dahurica and G.straminea,G.macrophylla and G.officinalis clustered together as sister clades,respectively.[Conclusion] The nucleotide differences of nrDNA ITS regions could be used for distinguishing botanical origin in Chinese herb Qingjiao.展开更多
Distribution of vegetation is closely coupled with climate; the climate controls distribution of vegetation and the vegetation type reflects regional climates. To reveal vegetation_climate relationships is the foundat...Distribution of vegetation is closely coupled with climate; the climate controls distribution of vegetation and the vegetation type reflects regional climates. To reveal vegetation_climate relationships is the foundation for understanding the vegetation distribution and theoretically serving vegetation regionalization. Vegetation regionalization is a theoretical integration of vegetation studies and provides a base for physiogeographical regionalization as well as agriculture and forestry regionalization. Based on a brief historical overview on studies of vegetation_climate relationships and vegetation regionalization conducted in China, we review the principles, bases and major schemes of previous vegetation regionalization and discuss on several contentious boundaries of vegetation zones in the present paper. We proposed that, under the circumstances that the primary vegetation has been destroyed in most parts of China, the division of vegetation zones/regions should be based on the distribution of primary and its secondary vegetation types and climatic indices that delimit distribution of the vegetation types. This not only reveals the closed relationship between vegetation and climate, but also is feasible practically. Although there still are divergence of views on the name and their boundaries of the several vegetation zones, it is commonly accepted that there are eight major vegetation regions in China, i.e. cold temperate needleleaf forest region, temperate needleleaf and broadleaf mixed forest region, warm temperate deciduous broadleaf forest region, subtropical evergreen broadleaf forest region, tropical monsoon forest and rain forest region, temperate steppe region, temperate desert region, and Qinghai_Xizang (Tibetan) Plateau high_cold vegetation region. Analyzing characteristics of vegetation and climate of major vegetation boundaries, we suggested that: 1) Qinling Mountain_Huaihe River line is an important arid/humid climatic, but not a thermal climatic boundary, and thus can not also be regarded as the northern limit of the subtropical vegetation zone; 2) the northern limit of subtropical vegetation zone in China is along the northern coast of the Yangtze River, from Hangzhou Bay, via Taihu Lake, Xuancheng and Tongling in Anhui Province, through by southern slope of the Dabie Mountains, to Wuhan and its west, coinciding with a warmth index ( WI ) value of 130-140 ℃·month; 3) the tropical region is limited in a very small area in southeastern Hainan Island and southern edge of Taiwan Island; and 4) considering a significant difference in climates between the southern and northern parts of the warm temperate zone, we suggested that the warm temperate zone in China is divided into two vegetation regions, deciduous broadleaf woodland region and deciduous and evergreen broadleaf mixed forest region, the Qinling Mountain_Huaihe River line being as their boundary. We also claimed that the zonal vegetation in North China is deciduous broadleaf woodland. Finally, we emphasized the importance of dynamic vegetation regionalization linked to climate changes.展开更多
Natural seedling regeneration and tree establishment are affected by various environmental factors. In this study, we established eight, eight, six, and four independent forest stands (each stand was further divided ...Natural seedling regeneration and tree establishment are affected by various environmental factors. In this study, we established eight, eight, six, and four independent forest stands (each stand was further divided into five subplots) respectively based on the altitudinal gradient, stand density, slope location, and slope aspect to investigate the effects of environmental factors on tree seedling regeneration in a pine-oak mixed forest. The results indicated that the seedling density was significantly higher at altitudes of 1,283 m to 1,665 m, whereas the sapling density did not differ with altitudes. The seedling and sapling density decreased significantly at 1,835 m. The seedling densities on the upper slopes were much higher than those on the middle and lower slopes, whereas the sapling density had no difference. The sapling density decreased southwest (20°-75°) whereas gradually from the it increased on the shady slopes to the northeast (40°). The seedling density increased from southwest (20°) to northeast (40°The seedling and sapling densities increased with the stand density (850 trees ha-1 to 1,525 trees ha-1) whereas the sapling density was significantly lower in stands (1,9oo trees ha-O. Principal components analysis showed that the slope aspect and stand density had more important roles in tree regeneration in this study region compared with the other two factors. Therefore, our findings suggest that it will be beneficial to keep stands at a moderate density on shady slopes. Appropriate thinning of higher density stands is also expected to promote the natural regeneration of pine-oak mixed forest.展开更多
AIM: To observe the biotransformation process of a Chinese compound, aesculin, by human gut bacteria, and to identify its metabolites in rat urine.METHODS: Representative human gut bacteria were collected from 20 he...AIM: To observe the biotransformation process of a Chinese compound, aesculin, by human gut bacteria, and to identify its metabolites in rat urine.METHODS: Representative human gut bacteria were collected from 20 healthy volunteers, and then utilized in vitro to biotransform aesculin under anaerobic conditions. At 0, 2, 4, 8, 12, 16, 24, 48 and 72 h postincubation, 10 mL of culture medium was collected. Metabolites of aesculin were extracted 3 × from rat urine with methanol and analyzed by HPLC. For in vivo metabolite analysis, aesculetin (100 mg/kg) was administered to rats via stomach gavage, rat urine was collected from 6 to 48 h post-administration, and metabolite analysis was performed by LC/ESI-MS and MS/MS in the positive and negative modes.RESULTS: Human gut bacteria could completely convert aesculin into aesculetin in vitro. The biotransformation process occurred from 8 to 24 h post-incubation, with its highest activity was seen from 8 to 12 h. The in vitro process was much slower than the in vivo process. In contrast to the in vitro model, six aesculetin metabolites were identified in rat urine, including 6-hydroxy-7-glucocoumarin(M1), 6-hydroxy-7-sulf-coumarin (M2), 6, 7-digluco-coumarin (M3), 6-glc-7-gluco-coumarin (M4), 6-O-methyl-7-gluco-coumarin (MS) and 6-O-methyl-7- sulf-coumarin (M6). Of which, M2 and M6 were novel metabolites.CONCLUSION: Aesculin can be transferred into aesculetin by human gut bacteria and is further modified by the host in vivo. The diverse metabolites of aesculin may explain its pleiotropic pharmaceutical effects.展开更多
基金The project was sponsored by the Scientific Re-search Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (1999-363).
文摘In July 2006, the diurnal activity rhythms of 13 heads of captive Qinling golden takin were observed in Shaanxi Provincial Centre for the Breeding and Conservation of Rare Wildlife (SPCBCRW) at daylight (from 9:00 am to 4:00 pm in July). The behavioral ethnogram was identified through observation at the sampling site, with the behaviors of golden takin recorded at 5 min interval using instantaneous scannable sampling method. The results indicated that the resting of golden takin took an important part in the daylight activities. Meanwhile, drinking and urinating and defecating were 1.92 and 0.54 and 0.92 times per head per day. Ambient temperature had a strong effect on the diurnal activity rhythms of golden takin. The diurnal activity rhymes were affected by ages of the animals effectively, which was expressed through differentiation of the time budgets. Moreover, different individuals in the same population showed some non-synchronously activity rhythms.
基金Supported by Science Foundation for the Excellent Youth and Middle-aged Scholars in Qinghai University(2009-QY-19)~~
文摘[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evaluation.[Method] The cpDNA psbA-trnH and nrDNA ITS sequences of five Chinese herb Qingjiao plants,including Gentiana macrophylla pall.,Gentiana straminea Maxim.,Gentiana crassicaulis Duthie ex Burk.,Gentiana dahurica Fisch and Gentiana officinalis H.Smith,were amplified with PCR,and then sequenced by direct PCR sequencing method for homologous analysis.[Results] The length of cpDNA psbA-trnH of five plants was 316-318 bp;there were seven different haplotypes and seven variable sites;the GC content of the sequence was 21.2%;the phylogenetic clustering showed the same result as haplotype analysis.The length of nrDNA ITS sequence of five plants was 624-625 bp,there were five different haplotypes and 13 variable sites;the GC content of the sequence was 59.3%.The result of phylogenetic clustering suggested that G.dahurica and G.straminea,G.macrophylla and G.officinalis clustered together as sister clades,respectively.[Conclusion] The nucleotide differences of nrDNA ITS regions could be used for distinguishing botanical origin in Chinese herb Qingjiao.
文摘Distribution of vegetation is closely coupled with climate; the climate controls distribution of vegetation and the vegetation type reflects regional climates. To reveal vegetation_climate relationships is the foundation for understanding the vegetation distribution and theoretically serving vegetation regionalization. Vegetation regionalization is a theoretical integration of vegetation studies and provides a base for physiogeographical regionalization as well as agriculture and forestry regionalization. Based on a brief historical overview on studies of vegetation_climate relationships and vegetation regionalization conducted in China, we review the principles, bases and major schemes of previous vegetation regionalization and discuss on several contentious boundaries of vegetation zones in the present paper. We proposed that, under the circumstances that the primary vegetation has been destroyed in most parts of China, the division of vegetation zones/regions should be based on the distribution of primary and its secondary vegetation types and climatic indices that delimit distribution of the vegetation types. This not only reveals the closed relationship between vegetation and climate, but also is feasible practically. Although there still are divergence of views on the name and their boundaries of the several vegetation zones, it is commonly accepted that there are eight major vegetation regions in China, i.e. cold temperate needleleaf forest region, temperate needleleaf and broadleaf mixed forest region, warm temperate deciduous broadleaf forest region, subtropical evergreen broadleaf forest region, tropical monsoon forest and rain forest region, temperate steppe region, temperate desert region, and Qinghai_Xizang (Tibetan) Plateau high_cold vegetation region. Analyzing characteristics of vegetation and climate of major vegetation boundaries, we suggested that: 1) Qinling Mountain_Huaihe River line is an important arid/humid climatic, but not a thermal climatic boundary, and thus can not also be regarded as the northern limit of the subtropical vegetation zone; 2) the northern limit of subtropical vegetation zone in China is along the northern coast of the Yangtze River, from Hangzhou Bay, via Taihu Lake, Xuancheng and Tongling in Anhui Province, through by southern slope of the Dabie Mountains, to Wuhan and its west, coinciding with a warmth index ( WI ) value of 130-140 ℃·month; 3) the tropical region is limited in a very small area in southeastern Hainan Island and southern edge of Taiwan Island; and 4) considering a significant difference in climates between the southern and northern parts of the warm temperate zone, we suggested that the warm temperate zone in China is divided into two vegetation regions, deciduous broadleaf woodland region and deciduous and evergreen broadleaf mixed forest region, the Qinling Mountain_Huaihe River line being as their boundary. We also claimed that the zonal vegetation in North China is deciduous broadleaf woodland. Finally, we emphasized the importance of dynamic vegetation regionalization linked to climate changes.
基金funded by the Special Research Program for Public-Welfare Forestry of State Forestry Administration of China (Grant No. 20100400206)National Natural Science Funds of China (Grant No. 31070570)CFERN & GENE Award Funds on Ecological Paper
文摘Natural seedling regeneration and tree establishment are affected by various environmental factors. In this study, we established eight, eight, six, and four independent forest stands (each stand was further divided into five subplots) respectively based on the altitudinal gradient, stand density, slope location, and slope aspect to investigate the effects of environmental factors on tree seedling regeneration in a pine-oak mixed forest. The results indicated that the seedling density was significantly higher at altitudes of 1,283 m to 1,665 m, whereas the sapling density did not differ with altitudes. The seedling and sapling density decreased significantly at 1,835 m. The seedling densities on the upper slopes were much higher than those on the middle and lower slopes, whereas the sapling density had no difference. The sapling density decreased southwest (20°-75°) whereas gradually from the it increased on the shady slopes to the northeast (40°). The seedling density increased from southwest (20°) to northeast (40°The seedling and sapling densities increased with the stand density (850 trees ha-1 to 1,525 trees ha-1) whereas the sapling density was significantly lower in stands (1,9oo trees ha-O. Principal components analysis showed that the slope aspect and stand density had more important roles in tree regeneration in this study region compared with the other two factors. Therefore, our findings suggest that it will be beneficial to keep stands at a moderate density on shady slopes. Appropriate thinning of higher density stands is also expected to promote the natural regeneration of pine-oak mixed forest.
基金Supported by Department of Traditional Chinese Medicine,Sichuan Province,No.03JY-002
文摘AIM: To observe the biotransformation process of a Chinese compound, aesculin, by human gut bacteria, and to identify its metabolites in rat urine.METHODS: Representative human gut bacteria were collected from 20 healthy volunteers, and then utilized in vitro to biotransform aesculin under anaerobic conditions. At 0, 2, 4, 8, 12, 16, 24, 48 and 72 h postincubation, 10 mL of culture medium was collected. Metabolites of aesculin were extracted 3 × from rat urine with methanol and analyzed by HPLC. For in vivo metabolite analysis, aesculetin (100 mg/kg) was administered to rats via stomach gavage, rat urine was collected from 6 to 48 h post-administration, and metabolite analysis was performed by LC/ESI-MS and MS/MS in the positive and negative modes.RESULTS: Human gut bacteria could completely convert aesculin into aesculetin in vitro. The biotransformation process occurred from 8 to 24 h post-incubation, with its highest activity was seen from 8 to 12 h. The in vitro process was much slower than the in vivo process. In contrast to the in vitro model, six aesculetin metabolites were identified in rat urine, including 6-hydroxy-7-glucocoumarin(M1), 6-hydroxy-7-sulf-coumarin (M2), 6, 7-digluco-coumarin (M3), 6-glc-7-gluco-coumarin (M4), 6-O-methyl-7-gluco-coumarin (MS) and 6-O-methyl-7- sulf-coumarin (M6). Of which, M2 and M6 were novel metabolites.CONCLUSION: Aesculin can be transferred into aesculetin by human gut bacteria and is further modified by the host in vivo. The diverse metabolites of aesculin may explain its pleiotropic pharmaceutical effects.