接种牛粪菌系对稻秸发酵特性及固液相菌群的影响

为考察接种牛粪菌系对稻秸的发酵特性和固液相菌群的影响,进行了批次试验。结果表明:牛粪菌系接种体系分别在第4天和第38天时出现了两个产甲烷峰,稻秸甲烷产率(以VS计)达269.32 m L/g,比对照体系提高了35%。纤维素酶和木聚糖酶活性分别达18.82、214.55 U/m L,使得干基质量降解率达41.79%。发酵结束后,细菌和甲烷菌群结构变化明显,稻秸固相上瘤胃球菌属(Ruminococcus)和纤维杆菌属(Fibrobacter)等纤维素水解菌相对丰度提高,分别与嗜氢型甲烷短杆菌属(Methanobrevibacter)和甲烷八叠球菌属(Methanosarcina)存在协同代谢,是牛粪菌系接种体系稻秸高效水解产甲烷的关键。和对照体系中存在的互营杆菌属(Syntrophobacter)和消化肠状菌属(Pelotomaculum)不同,接种体系的氨基杆菌属(Aminobacterium)和互营单胞菌属(Syntrophomonas)等互营氧化菌降低了丙酸和丁酸浓度,嗜乙酸产甲烷途径占主导优势,提高了厌氧发酵的效率。

Production of Biotechnology Fulvic Acid from Fermented Corn Straw by Microbial Community LCM9 and Its Application Effect

[Objective] The aim was to evaluate the feasibility of biotechnology fulvic acid produced by microbial community LCM9 by the use of corn straws as substrate and its application effect.[Method]Microbial community was obtained from the nature samples,and corn straws were used as fermentation substrate.The yield of biotechnology fulvic acid and its application effect was evaluated.[Result]The yield of biotechnology fulvic acid was up to 15.7% when the water content was 75.0% and fermented after 7 d using corn straws as the only source of nutrition.After 500 mg/kg of biotechnology fulvic acid was added for 5 d,the root length and bud length could be increased by 59.1% and 97.1%,respectively.[Conclusion]A higher yield of biotechnology fulvic acid could be obtained by the method in this experiment and the product could enhance the growth of wheat seedlings.

Study on Biogas Production Using Anaerobic Fermentation of Rice Straw

[Objective] The aim was to improve the biogas production rate,and improve the use ratio of rice straws to the furthest.[Method] Single-factor method was adopted to analyze the influence of inoculated amount,total solid content and carbon-nitrogen ratio on biogas production in biogas station of Nanjing University of Technology.[Result] It was found that the most optimum condition was：inoculated amount of 34%,total solid content of 6%,and carbon-nitrogen ratio of 25：1.According to the experimental verification,the total biogas yield in experimental group increased by 27.6% over the control group.[Conclusion] The research provided the reference of the further industrialization production of biogas using straw.

Effects of Dilute Acid-intensified Hydrolysis on Fermentative Biohydrogen Production Capacity of Maize Stalk

[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam explosion and dilute acid hydrolysis as experimental materials,we measured and analyzed the effects of different treatments and particle size of maize stalk were analyzed. [Result] The optimal fermentative biohydrogen production was found under following parameters：pretreatment of 0.8% dilute H2SO4 following steam explosion,particle size of maize stalk of 0.425-0.850 mm,liquid-solid ratio [0.8% H2SO4 （M）：stalk （W）] of 10：1. [Conclusion] Post steam explosion,dilute 0.8% dilute H2SO4 intensified hydrolysis on maize stalk could produce fermentative biohydrogen production capacity.

Status Quo of Collection and Utilization of Rice and Wheat Straw in Jiangsu Province and the Countermeasures

A case study of Jiangsu Province was conducted using questionnaires and field survey to explore the status quo of the collection and utilization of rice and wheat straw of the province. Problems in collecting the straw were analyzed taking into account meteorological data of the rice and wheat harvesting seasons in the region. Results show：（1） Currently, the main handling way of rice and wheat straw was directly returning to field. The peasant households of straw returning from investigated townships A and B respectively occupied 22.01% and 28.75% of investigation households, and both of the two townships had a considerable portion of the straw wasted or improperly disposed. In township B, over 50% of the farmer households surveyed failed to make sure of any of the straw, and as high as over55% of the straw was discarded or burnt.（2） During the rice and wheat harvesting seasons, the ten-day precipitation was among 7.21-87.28 mm, and the ten-day precipitation days were among 1.53-5.00 d. Such weather not only affected timely harvesting of rice and wheat, but also seriously impeded baling, transportation and storage of straw.（3） As the crops must be harvested in a relatively short and concentrated time period, large volumes of straw were turned out within a few days.Moreover, harvesting of the first crop often coincided with sowing, leaving little time available for straw collection.（4） Straw collection was very low in economic benefit.A farmer can only get 60-90 RMB per day from straw collection and transportation,which was much lower than what they can get by working as migrant workers in the city.（5） Machines designed for straw collection were rare and those now used in the operation were low in efficiency and needed to be improved technically,which directly affected the efficiency of straw collection. The above-listed findings indicates that time shortage, negative weather condition, low benefit and low mechanization level were major factors affecting straw collection. To solve the problems,the following proposals were brought forth, that is, improving the economic benefit of straw collection as a driving force, accelerating the research and development of rice and wheat sheaf-binding reaping machine, and setting up a long-term operational mechanism for straw recovery, in the hope that this study may provide some useful ideas to help solve the problems.

坚持抓好“无泄漏”工作

山西运城秸山化肥厂的生产装置均为七十年代中期投产项目。由于设备制造质量差,人员素质低,管理制度不健全,到了八十年代,设备明显老化,技术落后,后因缺乏资金而无法更新,造成设备故障多,跑、冒、滴、漏严重,不但腐蚀了设备,同时污染了环境,严重威胁着人身和设备安全。为彻底改变企业的被动局面,秸山化肥厂(简称秸化)从1987年开始,先后抓了“一类岗位”、“无泄漏区”、

设备管理的激励机制与约束机制

近年来,运城地区秸山化肥厂在设备管理工作中,着力于建立并初步形成了适应本企业实际状况的激励与约束办法,为保持设备完好、促进现场管理、巩固“无泄漏工厂”成果,保障生产正常进行创造了条件。他们的主要做法是:

节能与环保

1.造纸黑液资源化治污技术 造纸废液排放污染是我国一大环境公害,特别是目前中小型造纸厂没有废液处理措施,有些造纸黑液处理技术还不能彻底治理污染。有的治理方法需要很高的投资。该治污技术为秸化工程技术,它是一套完备的生产技术。

Research of Pyrolysis Oil and Catalysts of Garlic Stalks

We undertook a pyrolysis research on garlic stalks to solve its difficulty in use. We found that the inorganic salts in biomass played an important role in catalytic pyrolysis. Moreover, we studied the pyrolysis behavior under catalyst and catalyst-free conditions. The results showed that the weightlessness rate of garlic stalks was the highest at 550 ℃ under catalyst-free condition. The oil production rate increased with the increase of temperature and reached the peak at 550 ℃. Subsequently, as the temperature increased, the secondary cracking was intensified and oil production rate was decreased. The oil production rate increased significantly （almost 15%） under catalysis of KOH. However, the oil production rate was the highest at 500-600℃. What＇s more, we found that KOH played a major role in promoting decomposition of surface skin cells and subcutaneous fibrous layer of garlic stalks.

Allelopathic Potentials of Some Crop Residues on the Germination and Seedling Growth of Chromolaena Odoratum L.

The allelopathic effects of aqueous extracts of rice husk （Orza sativa L.） and sorghum stem （Sorghum bicolor L.） on the germination and seedling growth of Chromolaena odoratum L. were investigated. The extracts of the two crop residues exhibited inhibitory effects on the germination and seedling growth of Chromolaena odoratum L. The degree of retardation demonstrated in both extracts was concentration dependent. However the results obtained revealed that the retardation was more pronounced in the rice husk extract-treated seeds. However statistical analysis （P 〈 0.05% level） revealed that the degrees of inhibition of radicle and plumule obtained from various extracts treated seeds of both extracts were not significantly different when compared to those obtained from the control experiments as well as those obtained from the varying extract concentrations.

Wheat straw pretreatment with KOH for enhancing biomethane production and fertilizer value in anaerobic digestion

Wheat straw biodegradability during anaerobic digestion was improved by treatment with potassium hydroxide （KOH） to decrease digestion time and enhance biomethane production and fertility value. KOH concentrations of 1% （KI）, 3% （[（2）, 6% （K3） and 9% （l（4） were tested for wheat straw pretreatment at ambient temperature with a C：N ratio of 25：1.86% of total solids （TS）, 89% of volatile solids （VS） and 22% of lignocellulose, cellulose and hemi- cellulose （LCH） （22%） were decomposed effectively with the wheat straw pretreated by 6% KOH. Enhanced bio- gas production and cumulative biomethane yield of 258 ml. （g VS）-1 were obtained increased by 45% and 41% respectively, compared with untreated wheat straw. Pretreated wheat straw digestion also yielded a digestate with higher fertilizer values potassium （l 38%）, calcium （22%） and magnesium （16%）. These results show that TS, VS and LCH can be effectively removed from wheat straw pretreated with KOH, improving biodegradability biomethane production and fertilizer value.

Effects of Rice Straw Incorporation and Soil Pre－Flooding on the Fate of Applied （^（15）NH_4）_2SO_4－N and Growth of Rice

A pot experiment was conducted to investigate the effects of straw incorporation and soil pre-flooding on the fate of (￣(15)NH_4)_2SO_4-N and the growth of rice. Excessive application of rice straw when incorporated with (￣(15)NH_4)_2SO_4 at the C/N ratio of 40 reduced the loss of (￣(15)NH_4)_2SO_4-N and retarded the growth and development of rice significantly, while no adverse effects were observed on dry weight of panicle and the total recovery of (￣(15)NH_4)_2SO_4-N when rice straw was incorporated with (￣(15)NH_4)_2SO_4 at a C/N ratio less than 25. There were no significant effects of duration of soil pre-flooding within 6 weeks on (￣(15)NH_4)_2SO_4-Nuptake by rice and on rice growth, but, less loss of (￣(15)NH_4)_2SO_4-N was observed in the soil with a longer period of pre-flooding.

Effect of Garlic Straw on Physical and Chemical Characteristics of Continuous Cropping Soil and Root Activity of Tomato in Solar Greenhouse

Taking tomato ＂Kate two＂ as the experimental material, the effects of different dosage of garlic straw on physical and chemical characteristics of continuous cropping soil and root activity of tomato in solar greenhouse were studied. The results showed that after applying with garlic straw, maximal and minimal temperatures in 0-15 cm soil layers were directly proportional to the amount of returned garlic straw, which were 2.39 ℃ and 1℃ higher than that of the control respectively. The unit weight was decreasing upon the amount of applied garlic straws of soils in different depths, but the porosity of soil was increasing. For example, unit weight and porosity changed the most significantly in soils in the depths of 0-10 cm, which went down by 6.71% and 6.18%, respectively. Besides, garlic straw returning to field improved soil pH, reduced soil conductivity and soil acidification and relieved soil secondary salinization, especially in 0-20 cm soil layers. In addition to that, the application of garlic straw increased the root activity of tomato （max 31.45%）, and delayed root system senescence in late stage. Therefore, garlic straw returning could improve the physical and chemical characteristics of soil by succession planting and root activity of tomato in solar greenhouse.

Eco-materials Research — Study on Preparation and Properties of Woodceramics

A new technique has been developed to prepare woodceramics with the raw material of wheat straws.The partial properties of woodceramics such as the remains of carbon, the density, the porosity, the bending strength, and resistivity are tested at the same time. Systematic analyses of the material properties, the forming mechanism, and the regularity of woodceramics are made. The effects of the formula of raw materials, the consistencies of phenol resin, and the temperature, on the technique and the properties of woodceramics are displayed. It is shown that wheat straws can be used to produce woodceramics with this technique. The presented work provides useful ideas for the study of the preparation of woodceramics.

Pretreatment of Corn Stover Using Supercritical CO2 with Water-Ethanol as Co-solvent

Supercritical carbon dioxide,with water-ethanol as co-solvent,was applied to pretreat corn stover to enhance its enzymatic hydrolysis.The efficiency of pretreatment was evaluated by the final reducing sugar yield obtained from the enzymatic hydrolysis of cellulose.Under the operation conditions of pretreatment pressure 15 MPa,temperature 180 ℃ and time 1 h,the optimal sugar yield of 77.8℅ was obtained.Scanning electron microscopy(SEM) and chemical composition analysis were applied to the pretreated corn stover.The results showed that the surface morphology and microscopic structure of pretreated corn stover were greatly changed.After the pretreatment,the contents of hemicellulose and lignin were reduced obviously.Thus more cellulose was exposed,increasing the sugar yield.

Fate and Transformation of Nitrogen from ^(15)N-Labelled Rice Straw After Feeding Goat

Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.

Sustainable synthesis of 5-hydroxymethylfurfural from waste cotton stalk catalyzed by solid superacid-SO4^2-/ZrO2

A sustainable process was explored for the preparation of 5-hydroxymethylfurfural(HMF) by catalytic degradation of the waste cotton stalk. Solid super-acid(SO_4^(2-)/ZrO_2) was used as an efficient catalyst for the degradation of cotton stalk. Both decomposition experiments and kinetic study were conducted for the exploration of degradation condition and kinetics mechanism. The optimized experimental conditions are reaction temperature 503 K, reaction time 75 min and dosage of catalyst 30%(mass fraction) based on the decomposition experiments, under which a maximum yield of 27.2% for HMF could be achieved. Kinetic study was then carried out in the presence of SO_4^(2-)/ZrO_2. The theoretical results indicate that the activation energies for reducing sugar and HMF with catalyst are 96.71 k J/mol, 84.21 kJ/mol in the presence of SO_4^(2-)/ZrO_2, and they are 105.96 k J/mol and 119.37 k J/mol in the absence of SO_4^(2-)/ZrO_2.

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Effect of commercial cellulose enzymes was investigated by batch enzymatic hydrolysis at 15.0% （w/v） solid. It was found that the best commercial cellulose enzyme was Cellic（R） CTec comparing to Accellerase 1000TM and Accelerase 1500TM. The Cellic（R） CTec gave the highest reducing sugar concentration and rice straw conversion. Moreover, when the hydrolysate obtained from hydrolysis using Cellic（R） CTec was fermented by Saccharomyces cerevisiae TISTR 5596, it would give the highest ethanol. In this study, the Cellic（R） CTec was used for fed-batch prehydrolysis prior to ethanol production by simultaneous saccharification and fermentation （SSF） way at 20% （w/v） solid loading. It could produce 35.76 g/L or 4.6% （v/v） of ethanol concentration and 83.67 L/ton dry matter （DM） of yield.

Production Benefits of Double-Cropping Rice Under Optimized Application of Nitrogen and Potassium Fertilizers Combined with Chinese Milk Vetch and Straw Co-Returning to Fields

In order to explore the technology and effects of reducing nitrogen and potassium fertilizer applications in double-cropping rice,a field plot experiment was conducted to study the effects of optimized application of nitrogen and potassium fertilizers combined with returning Chinese milk vetch and straw to fields on yield,fertilizer utilization efficiency,net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci),chlorophyll content(SPAD value)and soil physical and chemical properties in late rice harvest period.The results showed that the optimized application of nitrogen and potassium fertilizers combined with the integrated technology of Chinese milk vetch and straw co-returning to the field could enhance the photosynthetic efficiency of double-cropping rice,increase rice yield,and enhance soil biological activity,especially T4 treatment involving the returning of Chinese milk vetch and straw to the field instead of 30%nitrogen fertilizer achieved the highest rice yield,fertilizer use efficiency,net photosynthetic rate and soil biological activity.Compared with the conventional fertilization treatment T2,the total rice yield of T4 treatment increased by 4.1%,among which the early rice and late rice increased by 6.3%and 2.4%,respectively;Pn,Gs and SPAD values of flag leaves at full heading stage significantly increased,and the contents of soil active organic carbon,alkali hydrolyzed nitrogen,available phosphorus and readily available potassium significantly increased.

Enhancing Biogas Production from Anaerobically Digested Wheat Straw Through Ammonia Pretreatment

Aqueous ammonia was used to pretreat wheat straw to improve biodegradability and provide nitrogen source for enhancing biogas production. Three doses of ammonia(2%, 4%, and 6%, dry matter) and three moisture contents(30%, 60%, and 80%, dry matter) were applied to pretreat wheat straw for 7 days. The pretreated wheat straws were anaerobically digested at three loading rates(50, 65, and 80 g·L-1) to produce biogas. The results indicated that the wheat straw pretreated with 80% moisture content and 4% ammonia achieved the highest methane yield of 199.7 ml·g-1(based on per unit volatile solids loaded), with shorter digestion time(T80) of 25 days at the loading rate of 65 g·L-1compared to untreated one. The main chemical compositions of wheat straw were also analyzed. The cellulose and hemicellulose contents were decomposed by 2%-20% and 26%-42%, respectively,while the lignin content was hardly removed, cold-water and hot-water extracts were increased by 4%-44%, and12%-52%, respectively, for the ammonia-pretreated wheat straws at different moisture contents. The appropriate C/N ratio and decomposition of original chemical compositions into relatively readily biodegradable substances will improve the biodegradability and biogas yield.