To investigate the effect of orthotopic liver transplantation on fulm inant hepatitis B and the preventive efficiency of lamivudine on recurrence of h epatitis B in China. Patients and methods. Ten patients with fulmi...To investigate the effect of orthotopic liver transplantation on fulm inant hepatitis B and the preventive efficiency of lamivudine on recurrence of h epatitis B in China. Patients and methods. Ten patients with fulminant hepatitis B received orthotopi c liver transplantation under veno venous bypass. All patients had preoperative ly serious jaundice, ascites and coagulopathy, and of whom 7 with encephalopathy , 2 with acute renal failure, and 1 with gastro hemorrhage. Rusult. Seven of the 10 patients have survived for 3～18 months, but 3 died of m ulti organ failure or recurrence of fulminant hepatitis B. Seven survivors took lamivudine and 6 of them have survived for 3～18 months without the signs of re currence of hepatitis B. Conclusion. Orthotopic liver transplantation is an effective therapy for fulmina nt hepatitis B, and lamivudine may prevent recurrence of hepatitis B after trans plantation.展开更多
Objective To investigate the anti-tumor effects in vitro and in vivo distribution of the human/murine chimeric antibody (D2C). Methods The CD71 positive target cells (K562, GEM and SMMC7721) and the effector cells, fr...Objective To investigate the anti-tumor effects in vitro and in vivo distribution of the human/murine chimeric antibody (D2C). Methods The CD71 positive target cells (K562, GEM and SMMC7721) and the effector cells, freshly isolated human PBMC, with the ratio of target cells to effector cells 1:50, were incubated in various dilutions of D2C antibody ( Ab) . Antibody dependent cytotoxicity (AD-CC) was tested by using an LDH-release assay. Instead of effector cells, complement was added to the target cells (GEM, SMMC-7721) with various dilutions of D2C Ab. A method of counting death cells was used in complement dependent cytotoxicity (CDC) assay. Tumor localization and distribution of the chimeric antibody (D2C) were observed by labeling the chimeric Ab with radioiodine(131I) and injecting it into nude mice (Balb/c nu/nu) transplanted with human hepatocellular carcinoma cells (SMMC-7721).Results A significant ADCC was observed with the increased concentration of the D2C Ab. Cytolysis of CD71-positive target cells by the D2C Ab was found in the presence of fresh rabbit complement. Labeled D2C administered by intraperitoneal as well as tumor regional injection, was visualized by SPECT. The distribution of D2C Ab in murine organs and tissues showed that non-specific binding was lower following tumor regional administration than when the antibody was administered by an intraperitoneal injection. The human/murine chimeric antibody (D2C) has in vitro anti-tumor effects and can exert its effects in specific tumor localization. Its distribution and local effects in vivo can be detected by radioimmunoimaging.Conclusion CD71 human/murine chimeric antibody showed marked killing of tumor cells in vitro, and specific recognition and high affinity binding to tumor tissue in vivo展开更多
This article looks at how population movements are addressed by the Sendai Framework for Disaster Risk Reduction 2015–2030(SFDRR), and highlights some of the potential implications of the SFDRR on disaster risk reduc...This article looks at how population movements are addressed by the Sendai Framework for Disaster Risk Reduction 2015–2030(SFDRR), and highlights some of the potential implications of the SFDRR on disaster risk reduction(DRR) and mobility management work. The article looks at the operational implications of the SFDRR text and covers issues of including migrants in DRR work;informing urban development about current and future mobility trends; managing relocations, evacuations, and displacement to prevent future risks and reduce existing ones; and preparing for and managing disaster-induced population movements to reduce the direct and indirect impacts of natural hazards. Overall, the references to human mobility within the SFDRR show an evolution in the way the issue is considered within global policy dialogues. Both the potential of population movements to produce risk and their role in strengthening the resilience of people and communities are now clearly recognized. This is an evolution of previously prevailing views of mobility as the consequence of disasters or as a driver of risk. While some implications of the DRR-mobility nexus might still be missing from DRR policy, population movements are now recognized as a key global risk dynamic.展开更多
文摘To investigate the effect of orthotopic liver transplantation on fulm inant hepatitis B and the preventive efficiency of lamivudine on recurrence of h epatitis B in China. Patients and methods. Ten patients with fulminant hepatitis B received orthotopi c liver transplantation under veno venous bypass. All patients had preoperative ly serious jaundice, ascites and coagulopathy, and of whom 7 with encephalopathy , 2 with acute renal failure, and 1 with gastro hemorrhage. Rusult. Seven of the 10 patients have survived for 3～18 months, but 3 died of m ulti organ failure or recurrence of fulminant hepatitis B. Seven survivors took lamivudine and 6 of them have survived for 3～18 months without the signs of re currence of hepatitis B. Conclusion. Orthotopic liver transplantation is an effective therapy for fulmina nt hepatitis B, and lamivudine may prevent recurrence of hepatitis B after trans plantation.
基金National Sciences Foundation of China(No.39970693)
文摘Objective To investigate the anti-tumor effects in vitro and in vivo distribution of the human/murine chimeric antibody (D2C). Methods The CD71 positive target cells (K562, GEM and SMMC7721) and the effector cells, freshly isolated human PBMC, with the ratio of target cells to effector cells 1:50, were incubated in various dilutions of D2C antibody ( Ab) . Antibody dependent cytotoxicity (AD-CC) was tested by using an LDH-release assay. Instead of effector cells, complement was added to the target cells (GEM, SMMC-7721) with various dilutions of D2C Ab. A method of counting death cells was used in complement dependent cytotoxicity (CDC) assay. Tumor localization and distribution of the chimeric antibody (D2C) were observed by labeling the chimeric Ab with radioiodine(131I) and injecting it into nude mice (Balb/c nu/nu) transplanted with human hepatocellular carcinoma cells (SMMC-7721).Results A significant ADCC was observed with the increased concentration of the D2C Ab. Cytolysis of CD71-positive target cells by the D2C Ab was found in the presence of fresh rabbit complement. Labeled D2C administered by intraperitoneal as well as tumor regional injection, was visualized by SPECT. The distribution of D2C Ab in murine organs and tissues showed that non-specific binding was lower following tumor regional administration than when the antibody was administered by an intraperitoneal injection. The human/murine chimeric antibody (D2C) has in vitro anti-tumor effects and can exert its effects in specific tumor localization. Its distribution and local effects in vivo can be detected by radioimmunoimaging.Conclusion CD71 human/murine chimeric antibody showed marked killing of tumor cells in vitro, and specific recognition and high affinity binding to tumor tissue in vivo
文摘This article looks at how population movements are addressed by the Sendai Framework for Disaster Risk Reduction 2015–2030(SFDRR), and highlights some of the potential implications of the SFDRR on disaster risk reduction(DRR) and mobility management work. The article looks at the operational implications of the SFDRR text and covers issues of including migrants in DRR work;informing urban development about current and future mobility trends; managing relocations, evacuations, and displacement to prevent future risks and reduce existing ones; and preparing for and managing disaster-induced population movements to reduce the direct and indirect impacts of natural hazards. Overall, the references to human mobility within the SFDRR show an evolution in the way the issue is considered within global policy dialogues. Both the potential of population movements to produce risk and their role in strengthening the resilience of people and communities are now clearly recognized. This is an evolution of previously prevailing views of mobility as the consequence of disasters or as a driver of risk. While some implications of the DRR-mobility nexus might still be missing from DRR policy, population movements are now recognized as a key global risk dynamic.
