AIM:To clarify the protective effect of exogenous adenosine triphosphate(ATP)on hypothermically preserved rat livers. METHODS:Establishment of continuous hypothermic machine perfusion model,detection of nucleotides in...AIM:To clarify the protective effect of exogenous adenosine triphosphate(ATP)on hypothermically preserved rat livers. METHODS:Establishment of continuous hypothermic machine perfusion model,detection of nucleotides in hepatocytes with HPLC,measurement of activities of LDH and AST in the perfusate,observation of histopathological changes in different experiment groups,and autoradiography were carried out to reveal the underlying mechanism of the protective effect of ATP. RESULTS:The intracellular levels of ATP and EC decreased rapidly after hypothermic preservation in control group,while a higher ATP and EC level,and a slower decreasing rate were observed when ATP-MgCl_2 was added to the perfusate (P<0.01).As compared with the control group,the activities of LDH and AST in the ATP-MgCl_2 group were lower(P<0.05). Furthermore,more severe hepatocyte damage and neutrophil infiltration were observed in the control group.Radioactive [α-^(32)P]ATP entered the hypothermically preserved rat hepatocytes. CONCLUSION:Exogenous ATP has a protective effect on rat livers during hypothermical preservation.However,Mg^(2+) is indispensable,addition of ATP alone produces no protective effect.The underlying mechanism may be that exogenous ATP enters the hypothermically preserved rat liver cells.展开更多
AIM： To investigate the relationship between donor liver cold preservation, lung surfactant （LS） changes and acute lung injury （ALI） after liver transplantation. METHODS： Liver transplantation models were estab-...AIM： To investigate the relationship between donor liver cold preservation, lung surfactant （LS） changes and acute lung injury （ALI） after liver transplantation. METHODS： Liver transplantation models were estab- lished using male Wistar rats. Donor livers were pre- served in University of Wisconsin solution at 4 ℃ for different lengths of time. The effect of ammonium pyr- rolidinedithiocarbamate （PDTC） on ALI was also detect- ed. All samples were harvested after 3 h reperfusion.The severity of AU was evaluated by lung weight/body weight ratio, lung histopathological score, serum nitric oxide （NO） and endothelin （ET）-I levels, lung tumor necrosis factor （TNF）-α and interleukin （IL）-1β levels. Lung surfactants （LSs） were determined by micellar electrokinetic capillary chromatography. RESULTS： With extended donor liver cold preservation time （CPT）, lung histopathological scores, serum ET-i levels, lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group （3.16± 0.28 vs 1.12 ± 0.21, P 〈 0.001; 343.59±53.97 vs 141.53± 48.48, P 〈 0.001; 0.00687 ±0.00037 vs 0.00557 ±0.00056, P 〈 0.001; 17.5 ± 3.0 vs 1,3 ± 0.3, P 〈 0.001; 10.8± 2.3 vs 1.8 ± 0.4, P 〈 0.001）, but serum NO levels decreased remarkably （74.67 ± 10.01 vs 24.97 ± 3.18, P 〈 0.001）. The expression of lung phos- phatidylcholine （PC）, phosphatidylethanolamine （PE）, phosphatidylinositol （PI） and phosphatidylserine （PS） increased when CPT was 〈 120 rain, and decreased when CPT was 〉 180 min （PC： 1318.89 ± 54.79 vs 1011.18± 59.99, P 〈 0.001; PE： 1504.45± 119.96 vs 1340.80±76.39, P = 0.0019; PI： 201.23 ± 34.82 vs 185.88 ± 17.04, P = 0.2265; PS： 300.43±32.95 vs 286.55± 55.55, P = 0.5054）. All these ALI-associated indexes could be partially reversed by PDTC treatment.展开更多
文摘AIM:To clarify the protective effect of exogenous adenosine triphosphate(ATP)on hypothermically preserved rat livers. METHODS:Establishment of continuous hypothermic machine perfusion model,detection of nucleotides in hepatocytes with HPLC,measurement of activities of LDH and AST in the perfusate,observation of histopathological changes in different experiment groups,and autoradiography were carried out to reveal the underlying mechanism of the protective effect of ATP. RESULTS:The intracellular levels of ATP and EC decreased rapidly after hypothermic preservation in control group,while a higher ATP and EC level,and a slower decreasing rate were observed when ATP-MgCl_2 was added to the perfusate (P<0.01).As compared with the control group,the activities of LDH and AST in the ATP-MgCl_2 group were lower(P<0.05). Furthermore,more severe hepatocyte damage and neutrophil infiltration were observed in the control group.Radioactive [α-^(32)P]ATP entered the hypothermically preserved rat hepatocytes. CONCLUSION:Exogenous ATP has a protective effect on rat livers during hypothermical preservation.However,Mg^(2+) is indispensable,addition of ATP alone produces no protective effect.The underlying mechanism may be that exogenous ATP enters the hypothermically preserved rat liver cells.
基金Supported by Grants from the National Natural Science Foundation of China,No.30200269Science and technology projects in Shannxi Province,No.2006k12-G3(6)Science and technology projects in Xi’an city,No.SF1025(3)
文摘AIM： To investigate the relationship between donor liver cold preservation, lung surfactant （LS） changes and acute lung injury （ALI） after liver transplantation. METHODS： Liver transplantation models were estab- lished using male Wistar rats. Donor livers were pre- served in University of Wisconsin solution at 4 ℃ for different lengths of time. The effect of ammonium pyr- rolidinedithiocarbamate （PDTC） on ALI was also detect- ed. All samples were harvested after 3 h reperfusion.The severity of AU was evaluated by lung weight/body weight ratio, lung histopathological score, serum nitric oxide （NO） and endothelin （ET）-I levels, lung tumor necrosis factor （TNF）-α and interleukin （IL）-1β levels. Lung surfactants （LSs） were determined by micellar electrokinetic capillary chromatography. RESULTS： With extended donor liver cold preservation time （CPT）, lung histopathological scores, serum ET-i levels, lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group （3.16± 0.28 vs 1.12 ± 0.21, P 〈 0.001; 343.59±53.97 vs 141.53± 48.48, P 〈 0.001; 0.00687 ±0.00037 vs 0.00557 ±0.00056, P 〈 0.001; 17.5 ± 3.0 vs 1,3 ± 0.3, P 〈 0.001; 10.8± 2.3 vs 1.8 ± 0.4, P 〈 0.001）, but serum NO levels decreased remarkably （74.67 ± 10.01 vs 24.97 ± 3.18, P 〈 0.001）. The expression of lung phos- phatidylcholine （PC）, phosphatidylethanolamine （PE）, phosphatidylinositol （PI） and phosphatidylserine （PS） increased when CPT was 〈 120 rain, and decreased when CPT was 〉 180 min （PC： 1318.89 ± 54.79 vs 1011.18± 59.99, P 〈 0.001; PE： 1504.45± 119.96 vs 1340.80±76.39, P = 0.0019; PI： 201.23 ± 34.82 vs 185.88 ± 17.04, P = 0.2265; PS： 300.43±32.95 vs 286.55± 55.55, P = 0.5054）. All these ALI-associated indexes could be partially reversed by PDTC treatment.
