Objective: To establish a swine model of orthotopic liver transplantation (OLT) which has high standardization, superior reproducibility and stability. Methods: The rate of success, reproducibility and stability were ...Objective: To establish a swine model of orthotopic liver transplantation (OLT) which has high standardization, superior reproducibility and stability. Methods: The rate of success, reproducibility and stability were investigated on the modification of OLTs in closed miniature swine with series of improvements. Results: 20 OLTs were performed on the basis of improvements in experimental animals, surgical procedures and operative monitorings. The mean operation time and anhepatic phase was (181±25.8) and (28.4±3.2) min respectively, which were significantly shorter than those of the previous reports. Liver function of the animals recovered shortly after operation. One-week survival rate was 90%, and 15 animals survived more than 1 month. The incidence of vascular and biliary complications was lower in animals with long-term survival. Conclusion: The improved animal model of OLTs in swine is easy to operate with high standardization and rate of success, superior reproducibility and stability. It is an ideal model for series studies related to liver transplantation in big animals.展开更多
With the development of oil and gas exploration industry, researchers and engineers have realized that the key element controlling the migration of underground oil and gas and other fluid is not the pressure of stratu...With the development of oil and gas exploration industry, researchers and engineers have realized that the key element controlling the migration of underground oil and gas and other fluid is not the pressure of stratum, but the underground fluid potential. Therefore, it is very crucial to study the distribution rule of fluid potential in order to correctly determine the exploration target areas. This paper studies the fluid potential distribution in Tertiary of west Qaidam Basin, puts forward the model of underground oil and gas migration and predicts the areas for further exploration.展开更多
文摘Objective: To establish a swine model of orthotopic liver transplantation (OLT) which has high standardization, superior reproducibility and stability. Methods: The rate of success, reproducibility and stability were investigated on the modification of OLTs in closed miniature swine with series of improvements. Results: 20 OLTs were performed on the basis of improvements in experimental animals, surgical procedures and operative monitorings. The mean operation time and anhepatic phase was (181±25.8) and (28.4±3.2) min respectively, which were significantly shorter than those of the previous reports. Liver function of the animals recovered shortly after operation. One-week survival rate was 90%, and 15 animals survived more than 1 month. The incidence of vascular and biliary complications was lower in animals with long-term survival. Conclusion: The improved animal model of OLTs in swine is easy to operate with high standardization and rate of success, superior reproducibility and stability. It is an ideal model for series studies related to liver transplantation in big animals.
文摘With the development of oil and gas exploration industry, researchers and engineers have realized that the key element controlling the migration of underground oil and gas and other fluid is not the pressure of stratum, but the underground fluid potential. Therefore, it is very crucial to study the distribution rule of fluid potential in order to correctly determine the exploration target areas. This paper studies the fluid potential distribution in Tertiary of west Qaidam Basin, puts forward the model of underground oil and gas migration and predicts the areas for further exploration.
