苣荬菜人工栽培技术

苣荬菜又称苦菜、甜苣、曲麻菜等,为菊科多年生草本植物,是我国食用历史悠久的一种野菜。随着“野菜热”的兴起,加上苣荬菜营养丰富,具有良好的保健价值,需求量逐渐增大,早春每公斤可买到12元左右,经济效益显著。苣荬菜可移根栽培,亦可直播栽培。

Study on Multiplication, Rooting and Transplanting of Tissue Culture Plantlets of Rhododendron chrysanthum Pall

[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pall tissue culture plantlets were explored. [Result] The medium composed of modified MS, iBA （3 mg/L） and ZT （1.5 mg/L） was the optimum medium for subculture mul- tiplication of Rh. chrysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium （150 mg/L）. [Conclusion] For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS （1/4） and IBA （5.0 mg/L）, and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of tufty soil, humus soil and perlite （2：1：1） with a survival rate of 95.66%.

Research on Plantlet Rooting of Lagerstroemia indica

Tissue culture plantlets of Lagerstroemia indica were used to study the effects of different auxins on in-vitro rooting, and the effects of various media in transplanting of rooted plantlets and ex-vitro rooting. For in-vitro rooting of test-tube plantiets of Lagerstroemia indica, 0.5 mg/L IBA is a suitable growth regulator; in the case of transplanting of rooted plantlets, the medium ratio of peat soil to perlite at 7：3 is better; and as to ex-vitro rooting of Lagerstroemia indica plantlets, the medium ratio of peat soil to perlite at 3：7 is more suitable.

Study on Tissue Culture and Rapid Propagation of Lycium ruthenicum Murr.

[Objective] This study aimed to establish a tissue culture and rapid propagation system for Lycium ruthenicum Murr.[Method] Using tender stems of L.ruthenicum as explants,MS as basic culture medium,the effects of different factors on primary culture,subculture and rooting of L.ruthenicum plantlets were investigated.[Result] The most appropriate medium for primary culture of L.ruthenicum was MS ＋ ZT 0.2 mg/L ＋ IBA 0.01 mg/L,in which axillary buds grew well and were rarely vitrified with the germination rate of 88.73%.In addition,ZT exerted significantly better effects on subculture and proliferation of L.ruthenicum plantlets than 6-BA.The most appropriate medium for subculture and proliferation of L.ruthenicum plantlets was MS ＋ ZT 0.15 mg/L ＋ IBA 0.01 mg/L,in which L.ruthenicum plantlets grew rapidly and robustly without vitrification,and the proliferation multiple reached 5.83 times.The most appropriate medium for rooting of L.ruthenicum plantlets was MS ＋ IBA 1.0 mg/L,in which the rooting rate reached 100%.The most appropriate substrate for transplanting and hardening of L.ruthenicum plantlets was humus soil：perlite = 1：1,in which L.ruthenicum plantlets grew well with the survival rate of 92.37%.[Conclusion] This study provided theoretical basis for largescale production and popularization of L.ruthenicum.