胶原蛋白与胎脑神经细胞移植入脑缺血大鼠的形态学研究

目的 观察胶原蛋白 (Collagen)与胎脑神经细胞 (FBN)联合培养 2天后 ,神经细胞与胶原蛋白的吸附情况 ;观察胶原蛋白与胎脑神经细胞 (CFBN)移植入脑缺血大鼠后神经细胞的生长情况。方法 取孕 14天的胎脑神经细胞接种在胶原蛋白上 ;制备大脑中动脉闭塞 (MCAO)模型 ;将CFBN植入到脑缺血大鼠中 ,将移植的大鼠分别在 2、6、10、30天进行光镜和电镜观察。结果 CFBN植入 2天后 ,机体产生轻微的免疫反应 ,部分中性粒细胞和淋巴细胞浸润 ,6天后免疫反应消失。HE和尼氏染色可见实验组植入神经细胞生长良好。结论 CFBN在脑缺血大鼠中生长良好 。

体外菲立磁标记恒河猴骨髓基质细胞自体移植入纹状体后磁共振对标记细胞形态学的示踪观察

目的:体外菲立磁标记的骨髓基质细胞经单细胞悬液微移植后,观察将其在恒河猴纹状体的存活、迁移、分化和整合状况,为细胞移植治疗疾病奠定基础。方法:实验于2004-04/2005-05在南方医科大学珠江医院全军神经医学研究所和解放军第四军医大学神经科学研究所完成。纳入健康恒河猴3只,离恒河猴骨髓基质细胞,利用神经干细胞培养基、白血病抑止分因子和碱性成纤维母细胞生长因子进行细胞扩增并诱导成骨髓基质源神经干细胞,再经体外菲立磁和活细胞荧光染料PK67标记后,采用微移植的方法,通过脑立体定位仪上用微玻璃针将干细胞分别植入脑纹状体内。细胞移植后1,4,8周应用核磁共振成像对脑内移植的细胞进行活体示踪,最后利用光镜和电镜观察标记细胞在脑内的形态学情况。结果:①体外菲立磁标记结果:骨髓基质细胞经微移植后可在脑内纹状体区域存活,移植的干细胞可向周围的脑实质内迁移和整合,迁移细胞沿特定的纹状体结构分布,少量细胞可分化成神经元。②核磁共振成像检查结果:发现脑内移植的标记细胞在磁共振上呈明显的低信号改变,未标记细胞侧脑组织无明显的低信号改变,与组织学切片结果基本相一致。结论:髓基质源神经干细胞移植后,可在脑内存活、迁移、分化和整合,骨利用核磁共振成像技术可以对脑内移植后的标记细胞进行初步的活体追踪。

菜苗移植入穴装置机械手的设计与研究

随着无土栽培这项新型现代农业技术的兴起,大规模种植农作物不再是天方夜谭,引入现代农业自动化设备能够大大减少人工工作强度以及提高移植效率,且可减少菜苗移植入穴的时间周期,降低菜苗的损坏率。本文在原先设计菜苗移植入穴装置的基础上,改进其机械爪的控制系统,实现菜苗移植入穴装置各个机构单元之间能够稳定可靠地协调配合运行,运用模糊PID控制实现系统的快速响应以及取得更高的精度和更快的调节时间,所设计的机械爪装置为农业现代化提供了一种全新的思路与方法。

菜苗移植入穴装置设计与研究

无土栽培是现代农业的一项新型的技术,是一项具有科技含量的技术形式;无土栽培适用于大规模种植农作物,在此期间就需要大规模引入现代农业自动化设备,针对无土栽培入穴一环,设计菜苗移植入穴装置整体机构以及尺寸,所设计的菜苗移植入穴能够降低无土栽培中菜苗移栽工作中的人工工作强度,在一定程度上解放劳动力,也为农业现代化提供了一种全新的方法。

胚中脑黑质移植入震颤麻痹模型鼠脑内的实验研究

胚中脑黑质移植入震颤麻痹模型鼠脑内的实验研究朱道立（江苏省南通师专生物系）采用６－羟基多巴胺（６－ＯＨＤＡ）分２点注入ＳＤ鼠右侧中脑黑质内侧端，制成震颤麻痹模型，毁损后６～８周，用胚龄１４～１６天同种胚鼠中脑黑质细胞悬液植入模型鼠尾壳核。实验分实验对...

美移植流产胎儿视网膜细胞治老人眼疾

美移植流产胎儿视网膜细胞治老人眼疾美国外科专家最近表示，他们已经把流产胎儿的视网膜细胞，成功地移植入１例患有视网膜斑点退化症老妇的眼内。如果移植效果良好，那么这种方法便会成为第一种对视网膜斑点退化症真正有效的疗法。据报道，该名８１岁的老妇，所患的视网...

科技信息两则

1、美国圣迭戈神经科学研究所埃文·巴拉班,将鹌鹑胚胎中的脑细胞取出,然后移植入鸡胚胎中并进行正常孵化,共培育出40只鹌鹑鸡。其外表与普通鸡无异,只是叫声和举动像鹌鹑。

Experience of Cervical Plexus Reinnervation for Patients with Unilateral Recurrent Laryngeal Nerve Invasion or Injury

OBJECTIVE To explore the clinical and therapeutic effects of cervical plexus reinnervation for infiltrated or injured unilateral recurrent laryngeal nerve （URLN）. METHODS Functional neck dissection for removal of differentiated thyroid carcinoma （DTC） in patients was performed, in which cervical plexus reinnervation was adopted for patients with stage I disease and URLN with injury or with tumor invasion. Outcomes of surgery were evaluated by examination under fibrolaryngoscope, and the patients＇ voices were evaluated before and after surgery. RESULTS All cases were followed up for 3 mon-2 years （average 8 mon）. Abductory motion of the vocal cords of 15 patients was completely or partly restored, but 3 patients＇ vocal cords were immovable. The recovery rate of abductory motion of the paralyzed vocal cords was 83.33% （15/18）. The function of phonation in the 16 patients was restored to normal or near normal limits, and their hoarseness was improved significantly. CONCLUSION Cervical plexus-URLN reinnervation should be considered when treating patients with unilateral vocal cord paralysis. Removing the tumor simultaneously with cervical plexus reinnervation during surgery for repair of unilateral recurrent laryngeal nerve injury was convenient and easy to perform with less functional damage compared with other methods of reinnervation. The abductory motion of vocal cord could be satisfactorily restored by this reinnervation. Surgical performance skills and application of neurotrophic drugs were important for the success of the surgery.

Effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmonary adenocarcinoma A549 cells

Objective： To evaluate the effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmo- nary adenocarcinoma A549 cells. Methods： Wound-healing and Transwell invasion assays were performed to study the effect of hypoxia on migration and invasion of A549 cells, and A549 cells were added to a monolayer of human umbilical vein endo-thelial cells （HUVECs） to test the ability to adhere to endothelium. Immunofluorescence assay and luciferase reporter gene assay were also used to evaluate the effect of hypoxia on distribution of E-cadherin, β-catenin, and actin, and hypoxia-inducible factor-1 （HIF-1）-dependent transcription, respectively. Results： Hypoxia facilitated A549 cell migration, invasion, and A549 cell-endothelial cells adhesion, and modulated the distribution of E-cadherin and β-catenin, and actin cytoskeleton rearrange-ment, and up-regulated HIF-l-dependent reporter gene expression in A549 cells. Conclusion： Promotion ofA549 cell migration, invasion, and adhesion on endothelium by hypoxia might be modulated through its up-regulating HIF-l-dependent gene expression, which then induced the redistribution of E-cadherin and β-catenin, and the actin cytoskeletal reorganization.

A wideband frequency-shift keying demodulator for wireless neural stimulation microsystems

This paper presents a wideband frequency-shift keying （FSK） demodulator suitable for a digital data transmission chain of wireless neural stimulation microsystems such as cochlear implants and retinal prostheses. The demodulator circuit derives a constant frequency clock directly from an FSK carrier, and uses this clock to sample the data bits. The circuit occupies 0.03 mm^2 using a 0.6 μm, 2M/2P, standard CMOS process, and consumes 0.25 mW at 5 V. This circuit was experimentally tested at transmission speed of up to 2.5 Mbps while receiving a 5-10 MHz FSK carrier signal in a cochlear implant system.

Unplanned hospital readmissions after kidney transplantation among patients in Hefei,China:Incidence,causes and risk factors

Objectives:Unplanned readmissions severely affect a patient's physical and mental well-being after kidney transplantation(KT),which is also independently associated with morbidity.A retrospective study was conducted to identify the incidence,causes and risk factors for unplanned readmission after KT among Chinese patients.Methods:Patients who underwent KT were admitted to the organ transplant center of the Affiliated Hospital of University of Science and Technology of China(2017-2018).Medical records for these patients were obtained through the hospital information system(HIS).Results:In 518 patients,the incidence of unplanned readmissions within 30 days(n=9)was 1.74%,and 90 days(n=64)was 12.35%.The one-year unplanned readmission rate was 22.59%(n=122).Overall,122 patients were readmitted because of infection,renal events,metabolic disturbances,surgical complications,etc.Hemodialysis(OR=10.462,95%CI:1.355-80.748),peritoneal dialysis(OR=8.746,95%CI:1.074-71.238)and length of stay(OR=1.023,95%CI:1.006-1.040)were independent risk factors for unplanned readmissions.Conclusion:Unplanned readmission rates increased with time after KT.Certain risk factors related to unplanned readmissions should be deeply excavated.Targeted interventions for controllable factors to alleviate the rate of unplanned readmissions should be identified.

Intervention of Mirtazapine on gemcitabine-induced mild cachexia in nude mice with pancreatic carcinoma xenografts

AIM: To investigate the effect of Mirtazapine on tumor growth, food intake, body weight, and nutritional status in gemcitabine-induced mild cachexia. METHODS: Fourteen mice with subcutaneous xenografts of a pancreatic cancer cell line (SW1990) were randomly divided into Mirtazapine and control groups. Either Mirtazapine (10 mg/kg) or saline solution was orally fed to the mice every day after tumor implantation. A model of mild cachexia was then established in both groups by intraperitoneal injection of Gemcitabine (50 mg/kg) 10 d, 13 d, and 16 d after tumor implanta- tion. Tumor size, food intake, body weight, and nutritional status were measured during the experiment. All mice were sacrificed at day 28. RESULTS: (1) After 7 d of gemcitabine administration, body-weight losses of 5%-7% which suggested mild cachexia were measured; (2) No significant difference in tumor size was detected between the Mirtazapine and control groups (P > 0.05); and (3) During the entire experimental period, food intake and body weight were slightly greater for the Mirtazapine group compared with controls (although these differences were not statistically significant). After 21 d, mice in the Mirtazapine group consumed significantly more food than control mice (3.95 ± 0.14 g vs 3.54 ± 0.10 g, P = 0.004). After 25 d, mice in the Mirtazapine group were also significantly heavier than control mice (17.24 ± 0.53 g vs 18.05 ± 0.68 g, P = 0.014). CONCLUSION: Mild cachexia model was successfully established by gemcitabine in pancreatic tumor-bearing mice. Mirtazapine can improve gemcitabine-induced mild cachexia in pancreatic tumor-bearing mice. It was believed to provide a potential therapeutic perspective for further studies on cachexia.

Research and porting of MiniGUI based on Embedded Linux System

With the development of embedded system, GUI becomes more and more important.As one of the most popular embedded GUI, MiniGUI became to be a key in researching the embedded GUI. This article firstly introduced embedded Linux system, processor ARM ,and S3C2410.Then, it analyses structures and primary predominances of MiniGUI. Finally, the transplantation of MiniGUI1.6.x based on $3C2410 is described in this paper.

Anteroposterior cricoid split interposition costal cartilage grafts for laryngotracheal stenosis in children

Objective: To investigate the effects of anteroposterior cricoid spliting interposition costal grafting for moderate and severe laryngotracheal stenosis in children. Methods: From 1995 to 2005, 87 children (aged 1. 2 to 14 years) with moderate and severe glottic and subglottic stenosis were retrospectively studied. They were operated with cricoid spliting laryngotracheal reconstruction in our hospital. All of 87 patients were tracheostomy-dependent before surgery. Results: Eighty-five patients (95%) were successfully decannnulated and got an effective phonation. The follow-up time was more than 5 years. The effect of operation was satisfactory and the growth and development of children was normal. Conclusion: Anteroposterior cricoid split interposition costal cartilage graft is a safe and effective treatment method for moderate and severe glottic and subglottic stenosis in children.

Retrospective analysis of exercise capacity in patients with coronary artery disease after percutaneous coronary intervention or coronary artery bypass graft

Objectives:To describe the current state of exercise capacity as well as to identify its predictors in patients with coronary artery disease (CAD) following percutaneous coronary intervention (PCI) or coronary artery bypass graft (CABG) in the mainland of China.Methods:A retrospective study design was employed.We evaluated 230 CAD patients following PCI or CABG in a cardiac rehabilitation center from January 2019 to October 2019.The patients were referred to undergo incremental cardiopulmonary exercise testing with a cycle ergometer.The Zung Self-Rating Anxiety Scale and the Zung Self-Rating Depression Scale were used to evaluate patients' mental health.Statistical analysis was performed using the chi-square test,Fisher's exact test,t-test,Mann-Whitney U test,and binary logistic regression.Results:Among the 230 patients,223 patients demonstrated reduced exercise capacity.Resutlts of the logistic regression analysis showed that anxiety (OR =1.13,95% CI 1.01-1.32,P =0.029) was an independent risk factor for reduced exercise capacity in patients following the PCI or CABG.Conclusions:Exercise capacity of Chinese CAD patients after PCI or CABG was relatively poor.Alleviating symptoms of anxiety and making exercise prescriptions according to the results of the cardiopulmonary exercise test should be considered during the intervention to improve CAD patients' exercise capacity.

Metabolic imaging for guidance of curative treatment of isolated pelvic implantation metastasis after resection of spontaneously ruptured hepatocellular carcinoma: A case report

Spontaneous rupture of hepatocellular carcinoma(HCC) is a life-threatening complication and its prognosis is significantly poor because of the high recurrence rate after initial hepatectomy. Resection of isolated extrahepatic metastasis of HCC has been advocated to obtain a possibility of long-term survival. However, it is a challenge for clinicians to detect implantation metastasis of spontaneously ruptured HCC. Accurate re-staging plays the most important role in making a decision on isolated metastasis resection. 18F-fluorodeoxyglucose(18F-FDG) positron emission tomography/computed tomography(PET/CT) is useful in detecting intraabdominal implantation metastasis from a variety of malignancies and shows superior accuracy to conventional imaging modalities in determining the location of metastasis. We present one patient with a new isolated pelvic implantation metastasis detected by 18F-FDG PET/CT and pathologically confirmed by PET/CT-guided percutaneous biopsy, who had a history of resection of spontaneously ruptured HCC two years ago. The patient's condition was stable at the 6-mo follow-up after resection of the isolated pelvic metastasis.

国产环孢素A眼液治疗大鼠角膜移植术后免疫排斥反应的研究

目的 观察环孢素A(cyclosporine,CsA)滴眼液对鼠角膜移植术后免疫排斥反应的治疗效果。方法 随机对纯系大鼠LOU 6 5只为受体及F344纯系大鼠 33只为供体建立的角膜移植术后免疫排斥反应动物模型分为 6组 ,各组分别于手术后滴用 0 5 %CsA、1 0 %CsA、2 0 %CsA、糖皮质激素、糖皮质激素和 1 0 %CsA及CsA基质滴眼液 ,观察角膜移植术后植片排斥反应指数 (rejectionindex,RI) ,植片平均存活时间 (meanssurvivaltime ,MST) ,并对角膜植片进行病理学检查及免疫病理学分析。结果 0 5 %CsA、1 0 %CsA、2 0 %CsA、糖皮质激素及混合组的植片MST均较对照组延长 1 6 3～ 8 46d ,其中 2 0 %CsA组、糖皮质激素组及混合组的植片MST延长与其他组比较差异有显著性 (t≥ 2 2 8,P <0 0 1) ,混合组为 (15 71± 5 0 6 )d。对照组的角膜植片排斥RI较其他组高 ,其中 2 0 %CsA组与糖皮质激素组的角膜植片排斥RI一致 ,分别为 5 34± 1 92和 5 18± 1 6 9;混合组治疗效果最好 ,RI为4 31± 1 5 0 ,而CsA滴眼液治疗后大鼠血清中的浓度介于 2 1 6～ 6 2 2mg/L。病理学检查 :各治疗组角膜植片淋巴细胞浸润减轻 ,新生血管减少 ;免疫病理显示 ,除 0 5 %CsA组外 ,其余各组的淋巴细胞相关抗原 1(LFA 1,CD+11a)、ICAM

雷公藤多甙及环孢素A在大鼠心脏移植中的协同作用

目的 探讨心脏移植术后单用雷公藤多甙 (TWHF)及与环孢素A(CsA)合用对大鼠心脏移植物生存期的影响。方法 供体为雄性PVG(RT1 C)大鼠 ,受体为雄性Lewis(LEW ;RT1 1)大鼠。采用改良的Ono和Lindsey方法实施腹部异位心脏移植。根据术后处理不同将实验动物分为 4组 :第 1组 (5只 ) :对照组 (非治疗组 ) ;第 2组 (7只 ) :TWHF 60mg·kg-1·d-1;第 3组 (6只 ) :CsA 1 5mg·kg-1·d-1;第 4组 (5只 ) :TWHF 40mg·kg-1·d-1+CsA 1mg·kg-1·d-1。用吐温 80及蒸馏水将TWHF配制成 1 %悬液 ;CsA使用前以注射用水配成 5g/L。术后当天即给药 ,TWHF为灌服 ,CsA为肌肉注射。直至发生排斥反应或最多给药 1 4d。每天通过触摸监测移植心脏的功能。结果 4组移植大鼠心脏平均存活 8.8、1 2 .0、1 7.0、3 4 .6d。结论 心脏移植术后给予雷公藤多甙及CsA联合治疗 。

磁共振成像在内耳畸形构成及术前评估中的应用

目的探讨磁共振成像(MRI)在内耳畸形诊断及分型中的应用价值,为感音神经性聋(sensorineural hearing loss,SNHL)患者提供人工耳蜗植入术(CI)前的可靠影像学资料。方法回顾性分析2014年8月~2017年12月间于我院就诊的314例(628耳)SNHL患者MRI水成像图像资料,采用Sennaroglu分类方法对内耳畸形患者进行诊断及分类,并分析SNHL患者的治疗情况。结果MRI对内耳畸形的检出率较高,内耳畸形中单纯前庭导水管畸形比率最高,半规管畸形比率次之,耳蜗畸形比率居第三位,内耳畸形患者的性别构成没有差异(P>0.05)。行CI的患者占所有就诊人数的47.77%。结论MRI作为一种无创检查技术可为患者内耳有无畸形及畸形的类型提供可靠的诊断信息,并为患者人工耳蜗植入术提供重要的影像资料。

肱骨远端骨不连围手术期的护理

目的:探索肱骨远端骨缺损与骨不连的显微外科修复后的护理方法。方法:采用解剖钢板固定为支架,取吻合血管的腓骨置入式移植方法,修复肱骨远端骨缺损与骨不连12例。术前加强心理护理与营养调理,术后着重进行患肢伤口周围、受区与供区的护理、出院前功能锻炼的指导等。结果:经围手术期的护理与术后功能锻炼的指导,无并发症发生,经3月～24个月随访,移植腓骨全部成活,退行变的肘关节面骨质恢复正常,肘关节仲屈,前臂旋转功能获得恢复,肢体的短缩获得纠正,持重也获得令人满意的效果。结论:本组邻近肘关节的肱骨远端骨不连均行外科手术2次～5次,硬化的骨质已累及肘关节的冠状窝,采用一般的吻合血管腓骨移植,无法获得固定,也可能进一步累及关节功能,而在解剖钢板固定支持下,吻合血管腓骨移植段可在相对稳定环境下获得愈合;该法护理简便,痛苦少,病程短,病人易于接受。