Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded conc...Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPl staining. Band expressions of Fas, Fas ligand, poly （ADP-ribose） polyrnerase （PARP） and D-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed. Results Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner. Conclusion Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.展开更多
A novel d!sphase supplying supported liquid membrane （DSSLM）, containing supplying feed phase andsupplying stripping phase tor transport behavior ot NI（Ⅱ）, have been studied. The supplying supported feed phase in...A novel d!sphase supplying supported liquid membrane （DSSLM）, containing supplying feed phase andsupplying stripping phase tor transport behavior ot NI（Ⅱ）, have been studied. The supplying supported feed phase included feed solution and di（2-ethyhexyl） phosphoric acid （HDEHP） as the carrier in kerosene, and supplying stripping phase included HDEHP as the cartier in kerosene and HC1 as the stripping agent. The effects of volume ratio of membrane solution to feed solution （O/F）, pH, initial concentration of Ni（Ⅱ） and ionic strength in the feedsolution, volume ratio of membrane solution to stripping solution （O/S）, concentration of H2SO4 solution, HDEHP concentration in the supplying stripping phase on transport of Ni（/I）, the advantages of DSSLM compared to the traditional supported liquid membrane （SLM）, the system stability, the reuse of membrane solution and the reten- tion of membrane phase were studied. Experimental results indicated that the optimum transpgrt of Ni（Ⅱ） was oh-tained when H2SO4 concentration was 2.00 mol＇L-＇, HDEHP concentration was 0.120 mol·L-1, and O/S was 4· 1 in the supplying stripping phase, O/F was 1 ： 10 and pH was 5.20 in the supplying feed phase. The ionic strength in supplying feed phase had no obvious effect on transport of Ni（Ⅱ）. When initial Ni（Ⅱ） concentration was 2.00x 10-4 mol/L, the transport percentage of Ni（Ⅱ） was up to 93.1% in 250 min. The kinetic equation was deduced in terms of the law of mass diffusion and the interface chemistry.展开更多
基金Supported by the Beijing Natural Science Foundation(7102128)
文摘Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPl staining. Band expressions of Fas, Fas ligand, poly （ADP-ribose） polyrnerase （PARP） and D-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed. Results Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner. Conclusion Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.
基金Supported by the Action Plan for the Development of Western China of the Chinese Academy of Sciences(KZCX2-XB2-13)the Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-YW-N-003)Research Fund for Excellent Doctoral Thesis of Xi'an University of Tehcnology(602-210805 and 602-210804)
文摘A novel d!sphase supplying supported liquid membrane （DSSLM）, containing supplying feed phase andsupplying stripping phase tor transport behavior ot NI（Ⅱ）, have been studied. The supplying supported feed phase included feed solution and di（2-ethyhexyl） phosphoric acid （HDEHP） as the carrier in kerosene, and supplying stripping phase included HDEHP as the cartier in kerosene and HC1 as the stripping agent. The effects of volume ratio of membrane solution to feed solution （O/F）, pH, initial concentration of Ni（Ⅱ） and ionic strength in the feedsolution, volume ratio of membrane solution to stripping solution （O/S）, concentration of H2SO4 solution, HDEHP concentration in the supplying stripping phase on transport of Ni（/I）, the advantages of DSSLM compared to the traditional supported liquid membrane （SLM）, the system stability, the reuse of membrane solution and the reten- tion of membrane phase were studied. Experimental results indicated that the optimum transpgrt of Ni（Ⅱ） was oh-tained when H2SO4 concentration was 2.00 mol＇L-＇, HDEHP concentration was 0.120 mol·L-1, and O/S was 4· 1 in the supplying stripping phase, O/F was 1 ： 10 and pH was 5.20 in the supplying feed phase. The ionic strength in supplying feed phase had no obvious effect on transport of Ni（Ⅱ）. When initial Ni（Ⅱ） concentration was 2.00x 10-4 mol/L, the transport percentage of Ni（Ⅱ） was up to 93.1% in 250 min. The kinetic equation was deduced in terms of the law of mass diffusion and the interface chemistry.
