Hydrolysis activities of PACC (particle of agarose-Ce4+ complex, newly made through double emulsification) forcompounds containing phosphodiester or peptide bonds were studied. The results showed that PACC could hydro...Hydrolysis activities of PACC (particle of agarose-Ce4+ complex, newly made through double emulsification) forcompounds containing phosphodiester or peptide bonds were studied. The results showed that PACC could hydrolyzeorganophosphorous pesticides not only in water but also in vegetable juice or tea extract. Hydrolysis rates of methamidophos,omethoate and chlorpyrifos in water are 32.39%, 27.12% and 46.62% respectively, those of chlorpyrifos and methami-dophos in mung sprout juice 38.28% and 35.45% respectively, and that of chlorpyrifos in tea extract 59.76%. Hydrolysisrates of BSA (bovine serum albumin) in water and protein in tea extract by PACC increase by 54.30% and 86.46% respec-tively as compared with the control.展开更多
NF-κB signaling controls a large set of physiological processes ranging from inflammatory responses to cell death. Its activation is tightly regulated through controlling the activity and stability of multiple signal...NF-κB signaling controls a large set of physiological processes ranging from inflammatory responses to cell death. Its activation is tightly regulated through controlling the activity and stability of multiple signaling components. Here, we identify that NF-κB activation is suppressed by an F-box protein, S-phase kinase associated protein 2 （SKP2）. SKP2 deficiency enhanced NF-κB activation as well as the production of inflammatory cytokines. In addition, SKP2 potently blocked the NF-κB activation at the κB kinase （IKIO level. Mechanistic study further revealed that SKP2 functions as an adaptor to promote an interaction between active IKKβ and the autophagic cargo receptor p62 to mediate IKKβ degradation via selective autophasy. These findings identify a previously unrecognized role of SKP2 in NF-κB activation by which SKP2 acts as a secondary receptor to assist IKKβ delivery to autophagosomes for degradation in a p62-dependent manner.展开更多
基金supported by the R and D Project Fund of Qingdao(03-2-HH-3)the Natural Science Foundation of Shandong(Z 2004D05)
文摘Hydrolysis activities of PACC (particle of agarose-Ce4+ complex, newly made through double emulsification) forcompounds containing phosphodiester or peptide bonds were studied. The results showed that PACC could hydrolyzeorganophosphorous pesticides not only in water but also in vegetable juice or tea extract. Hydrolysis rates of methamidophos,omethoate and chlorpyrifos in water are 32.39%, 27.12% and 46.62% respectively, those of chlorpyrifos and methami-dophos in mung sprout juice 38.28% and 35.45% respectively, and that of chlorpyrifos in tea extract 59.76%. Hydrolysisrates of BSA (bovine serum albumin) in water and protein in tea extract by PACC increase by 54.30% and 86.46% respec-tively as compared with the control.
基金This work was supported by grants from the National Natural Science Foundation of China （91629101, 31522018, 31601135, 81302197, 81700557, and 31071046）, the National Key Basic Research Program of China （2015CB859800 and 2014CB910800）, and the 6uangdong Innovative Research Team Program （2011Y035）.
文摘NF-κB signaling controls a large set of physiological processes ranging from inflammatory responses to cell death. Its activation is tightly regulated through controlling the activity and stability of multiple signaling components. Here, we identify that NF-κB activation is suppressed by an F-box protein, S-phase kinase associated protein 2 （SKP2）. SKP2 deficiency enhanced NF-κB activation as well as the production of inflammatory cytokines. In addition, SKP2 potently blocked the NF-κB activation at the κB kinase （IKIO level. Mechanistic study further revealed that SKP2 functions as an adaptor to promote an interaction between active IKKβ and the autophagic cargo receptor p62 to mediate IKKβ degradation via selective autophasy. These findings identify a previously unrecognized role of SKP2 in NF-κB activation by which SKP2 acts as a secondary receptor to assist IKKβ delivery to autophagosomes for degradation in a p62-dependent manner.
