期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
稻瘟病菌单克隆抗体的研制及其对稻瘟病菌附着胞形成的影响 被引量:19
1
作者 吴建祥 林福呈 +2 位作者 李德葆 陈正贤 娄沂春 《微生物学报》 CAS CSCD 北大核心 2000年第6期638-645,共8页
稻温病菌的分生孢子、芽管、附着胞的混合物作为抗原免疫BALB/c小鼠 ,取免疫小鼠的脾细胞与SP2 /0骨髓瘤细胞在 50 %PEG下融合成杂交瘤细胞 ,用间接ELISA筛选阳性孔 ,获 1 1株单克隆抗体。间接免疫荧光试验表明其中 4株单克隆抗体 2B4、... 稻温病菌的分生孢子、芽管、附着胞的混合物作为抗原免疫BALB/c小鼠 ,取免疫小鼠的脾细胞与SP2 /0骨髓瘤细胞在 50 %PEG下融合成杂交瘤细胞 ,用间接ELISA筛选阳性孔 ,获 1 1株单克隆抗体。间接免疫荧光试验表明其中 4株单克隆抗体 2B4、4A1 、1D1 和 2H4分别与孢子、芽管或附着胞有特异性结合 ;Westernblotting分析发现 2B4、4A1 、1D1 单克隆抗体分别与孢子、芽管表面的提取物有不同的结合带 ;此四株单克隆抗体均干扰稻温病菌附着胞形成 ,并抑制稻温病菌在叶表的致病性。 展开更多
关键词 稻瘟病菌附着胞 单克隆抗体 间接免疫荧光试验
下载PDF
Antifungal Effect of Streptomyces 702 Antifungal Monomer Component DZP8 on Rhizoctonia solani and Magnaporthe grisea 被引量:1
2
作者 芦慧 涂晓嵘 +2 位作者 徐佳 冯华根 涂国全 《Agricultural Science & Technology》 CAS 2012年第11期2370-2374,2378,共6页
The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia ... The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia solani and on the mycelium growth, conidial formation, germination, appressorium formation of Magnaporthe grisea. The results showed that the antifungal monomer component DZP8 has strong antifungal effect on both the R. solani and M. grisea. The EC50 and EC90 of DZP8 were 1.81 and 3.35 μg/ml on Ft. solani respectively, and 37.01 and 136.21 μg/ml on M. grisea respectively. Under the treatment of 48.01 μg/ml DZP8, the sclerotium formation rate of R. solani was just 39.21%, the formation time delayed by 216 h and the dry weight decreased by 81.37% in comparison the con- trol; and 33.51 μg/ml DZP8 significantly inhibited the sclerotium germination. In the presence of 160.08 μg/ml DZP8, the sporulation of M. grisea was just 9.29% of control sample; 20.14 μg/ml DZP8 inhibited the conidial germination suppression rate by 95.16%, and the appressorium formation by 100%. 展开更多
关键词 Streptomyces 702 Monomer component DZP8 Rhizoctonia solani Mag-naporthe grisea Antifungal effect
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部