从稻田腐烂秸秆中分离到一批纤维素分解菌株。通过滤纸崩解测试、刚果红纤维素平板识别,以及产酶鉴定,筛选得到一株分解纤维素能力较强的真菌。经形态观察和18S r DNA基因片断分析,鉴定该菌株为青霉。对菌株的液态发酵条件进行研究,该...从稻田腐烂秸秆中分离到一批纤维素分解菌株。通过滤纸崩解测试、刚果红纤维素平板识别,以及产酶鉴定,筛选得到一株分解纤维素能力较强的真菌。经形态观察和18S r DNA基因片断分析,鉴定该菌株为青霉。对菌株的液态发酵条件进行研究,该菌株培养基含3%稻草粉、0.25%尿素和无机盐营养液,最佳产酶条件为:自然pH,30℃,130r/min发酵4d。该菌株的CMC酶活和滤纸酶活最高分别达到45.01I U/mL和6.89I U/mL。随后对该菌酶解稻草粉进行研究,糖化率达到40.17%。研究表明,青霉T24-2菌株在秸秆综合利用上具有良好的应用前景。展开更多
The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride ( [ AMIM ] C1) is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice s...The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride ( [ AMIM ] C1) is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice straw and improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows: the dosage of rice straw in [ AMIM ] Cl, crush mesh of rice straw, pretreatment temperature and time. After the pretreatment with a 3 % (the weight ratio of rice straw to ionic liquid) rice straw dosage in [AMIM]Cl at 110 ℃ for 1 h, the yield of reducing sugar of regenerated rice straw by 33 U/mL cellulase hydrolysis was 53.3 %, which was two times higher than that of un-treated rice straw (23.7 % ). More researches regarding straw biorefinery to bacterial cellulose are being performed in the lab and prospective results will be published in near future.展开更多
文摘从稻田腐烂秸秆中分离到一批纤维素分解菌株。通过滤纸崩解测试、刚果红纤维素平板识别,以及产酶鉴定,筛选得到一株分解纤维素能力较强的真菌。经形态观察和18S r DNA基因片断分析,鉴定该菌株为青霉。对菌株的液态发酵条件进行研究,该菌株培养基含3%稻草粉、0.25%尿素和无机盐营养液,最佳产酶条件为:自然pH,30℃,130r/min发酵4d。该菌株的CMC酶活和滤纸酶活最高分别达到45.01I U/mL和6.89I U/mL。随后对该菌酶解稻草粉进行研究,糖化率达到40.17%。研究表明,青霉T24-2菌株在秸秆综合利用上具有良好的应用前景。
基金Science and Technology Commission of Shanghai Municipality (No.11230700600No.08520750200)+2 种基金Shanghai Municipal Education Commission (No.09ZZ68)the "111 " Project(No.B07024)the Fundamental Research Funds for the Central Universities and Key Laboratory of Science &Technology of Eco-Textile (Donghua University),Ministry of Education (No.Eco-op-2009-09)
文摘The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride ( [ AMIM ] C1) is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice straw and improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows: the dosage of rice straw in [ AMIM ] Cl, crush mesh of rice straw, pretreatment temperature and time. After the pretreatment with a 3 % (the weight ratio of rice straw to ionic liquid) rice straw dosage in [AMIM]Cl at 110 ℃ for 1 h, the yield of reducing sugar of regenerated rice straw by 33 U/mL cellulase hydrolysis was 53.3 %, which was two times higher than that of un-treated rice straw (23.7 % ). More researches regarding straw biorefinery to bacterial cellulose are being performed in the lab and prospective results will be published in near future.