VacA was histochemically stained in biopsy specimen and was intracellularly an d mainly located in fundic gland area. It is recognized gastric atrophy was obse rved in the H. pylori-positive patients with intracellula...VacA was histochemically stained in biopsy specimen and was intracellularly an d mainly located in fundic gland area. It is recognized gastric atrophy was obse rved in the H. pylori-positive patients with intracellular VacA compared with o thers.The aim of study is to understand the relationship between intracellular V acA and the progression of gastric atrophy that is associated with gastric cance r. Biopsy specimens and sera were obtained from 364 people in their 50s and 60s without gastric cancer diagnosed at first endoscopy undergoing diagnostic endosc opy, for H. pylori infection, histology, and the histochemical status of intrace llular VacA using anti-VacA Ab during the follow-up period (mean, 7.3 years). Three hundred eleven of 364 enrolled patients were H. pylori positive and 53 pat ients were H. pylori negative at first endoscopy. VacA was intracellularly stain ed with vacuolation and cell de struction in the fundic gland in 98 of 311 H. pylori-positive patients and no t stained in another 213 H. pylori-positive patients plus 53 H.pylori-negative patients at first endoscopy. Gastric atrophy has significantly progressed in th e H. pylori-positive patints with intracellular VacA with gastric ulcers compar ed with the others and six gastric cancers have developed in this group during t he follow-up period (mean, 7.3 years). Intracellular VacA is a valuable marker to predict whether Helicobacter pylori induces progressive atrophic gastritis th at is associated with the development of gastric cancer.展开更多
目的:观察黄芪含药血清能否有效抑制体外幽门螺杆菌(hel i cobact er pyl ori,Hp)空泡毒素(VacA)的活性,从而探讨其抗菌机制。方法:将HP菌株培养上清液制成粗制VacA蛋白,经倍比稀释后(1∶32～1∶1),选择1∶2滴度与不同浓度黄芪含药血清...目的:观察黄芪含药血清能否有效抑制体外幽门螺杆菌(hel i cobact er pyl ori,Hp)空泡毒素(VacA)的活性,从而探讨其抗菌机制。方法:将HP菌株培养上清液制成粗制VacA蛋白,经倍比稀释后(1∶32～1∶1),选择1∶2滴度与不同浓度黄芪含药血清共同作用于胃癌SGC-7901细胞,观察胃癌细胞形态变化,采用中性红摄入法(NRU)评价空泡活性。结果:黄芪含药血清对VacA活性有显著抑制作用,含药血清小剂量组NRU检测其空泡活性A550=0.228±0.029,显微镜下计数空泡形成率为78.75%±8.539%,与阴性对照组比较差异有显著性(P<0.05),随血清含药浓度的增加A550和空泡形成率逐渐降低;含药血清大剂量组A550=0.127±0.047,空泡形成率为32.25%±10.21%,与阴性对照组比较差异有极显著性(P<0.01),与阳性对照奥美拉唑组比较差异无显著性(P>0.05);含药血清大剂量组与中剂量组A550比较,差异无显著性(P>0.05),与小剂量组比较差异有显著性(P<0.05),可见黄芪含药血清对空泡毒素的抑制作用具有浓度依赖性。结论:黄芪对HP VacA活性具有明显的抑制作用,且该抑制作用具有一定的浓度依赖性。展开更多
目的 :研究幽门螺杆菌空泡毒素作为单一毒力决定簇对真核细胞的作用。方法 :用 PCR扩增vac A基因片段 ,克隆入真核表达载体 p EGFP- N 1,构建重组质粒 p EGFP- vac A,转染 He L a细胞 ,通过相差显微镜和电子显微镜观察细胞形态与结构的...目的 :研究幽门螺杆菌空泡毒素作为单一毒力决定簇对真核细胞的作用。方法 :用 PCR扩增vac A基因片段 ,克隆入真核表达载体 p EGFP- N 1,构建重组质粒 p EGFP- vac A,转染 He L a细胞 ,通过相差显微镜和电子显微镜观察细胞形态与结构的变化。结果 :重组质粒转染 He L a细胞 2 4 h,10 %～ 2 0 %细胞的胞质内出现明显空泡 ,其中少数细胞发生凋亡改变。结论 :成功构建了用于真核表达的重组 vac A质粒 ,转染真核细胞后 ,观察 Vac A作用导致的细胞形态结构的变化 ,为研究 Vac展开更多
文摘VacA was histochemically stained in biopsy specimen and was intracellularly an d mainly located in fundic gland area. It is recognized gastric atrophy was obse rved in the H. pylori-positive patients with intracellular VacA compared with o thers.The aim of study is to understand the relationship between intracellular V acA and the progression of gastric atrophy that is associated with gastric cance r. Biopsy specimens and sera were obtained from 364 people in their 50s and 60s without gastric cancer diagnosed at first endoscopy undergoing diagnostic endosc opy, for H. pylori infection, histology, and the histochemical status of intrace llular VacA using anti-VacA Ab during the follow-up period (mean, 7.3 years). Three hundred eleven of 364 enrolled patients were H. pylori positive and 53 pat ients were H. pylori negative at first endoscopy. VacA was intracellularly stain ed with vacuolation and cell de struction in the fundic gland in 98 of 311 H. pylori-positive patients and no t stained in another 213 H. pylori-positive patients plus 53 H.pylori-negative patients at first endoscopy. Gastric atrophy has significantly progressed in th e H. pylori-positive patints with intracellular VacA with gastric ulcers compar ed with the others and six gastric cancers have developed in this group during t he follow-up period (mean, 7.3 years). Intracellular VacA is a valuable marker to predict whether Helicobacter pylori induces progressive atrophic gastritis th at is associated with the development of gastric cancer.
文摘目的:观察黄芪含药血清能否有效抑制体外幽门螺杆菌(hel i cobact er pyl ori,Hp)空泡毒素(VacA)的活性,从而探讨其抗菌机制。方法:将HP菌株培养上清液制成粗制VacA蛋白,经倍比稀释后(1∶32～1∶1),选择1∶2滴度与不同浓度黄芪含药血清共同作用于胃癌SGC-7901细胞,观察胃癌细胞形态变化,采用中性红摄入法(NRU)评价空泡活性。结果:黄芪含药血清对VacA活性有显著抑制作用,含药血清小剂量组NRU检测其空泡活性A550=0.228±0.029,显微镜下计数空泡形成率为78.75%±8.539%,与阴性对照组比较差异有显著性(P<0.05),随血清含药浓度的增加A550和空泡形成率逐渐降低;含药血清大剂量组A550=0.127±0.047,空泡形成率为32.25%±10.21%,与阴性对照组比较差异有极显著性(P<0.01),与阳性对照奥美拉唑组比较差异无显著性(P>0.05);含药血清大剂量组与中剂量组A550比较,差异无显著性(P>0.05),与小剂量组比较差异有显著性(P<0.05),可见黄芪含药血清对空泡毒素的抑制作用具有浓度依赖性。结论:黄芪对HP VacA活性具有明显的抑制作用,且该抑制作用具有一定的浓度依赖性。
