This study on the temporal and spatial variability of the viscosity and some chemical parameters in the sea surface microlayer (SML), the relationship between the viscosity and chemical parameters, and the influence o...This study on the temporal and spatial variability of the viscosity and some chemical parameters in the sea surface microlayer (SML), the relationship between the viscosity and chemical parameters, and the influence of the viscosity on the mass transfer coefficient ( K ) in the flux of materials through the air sea interface revealed that: The values of viscosity and some chemical parameters in the SML are higher than those in the sub surface layer (SSL), and at daytime are higher than those at night. The viscosity has positive corelation with chemical oxygen demand (COD), dissolved organic carbon (DOC) and salinity. The "SML effect" on K need not be considered because the SML effect on materials concentration is so small.展开更多
The fourth-order B spline wavelet scaling functions are used to solve the two-dimensional unsteady diffusion equation. The calculations from a case history indicate that the method provides high accuracy and the compu...The fourth-order B spline wavelet scaling functions are used to solve the two-dimensional unsteady diffusion equation. The calculations from a case history indicate that the method provides high accuracy and the computational efficiency is enhanced due to the small matrix derived from this method.The respective features of 3-spline wavelet scaling functions,4-spline wavelet scaling functions and quasi-wavelet used to solve the two-dimensional unsteady diffusion equation are compared. The proposed method has potential applications in many fields including marine science.展开更多
Two-dimensional electrophoresis(2-DE) of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kD...Two-dimensional electrophoresis(2-DE) of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa-31 kDa and 43 kDa-66 kDa, and more proteins were detected between 14kDa and 31 kDa. With the improved protein preparation, the two-dimensional electrophoresis patterns indicated that the relative molecular weights of the proteins were between 14kDa and 100kDa, and most of them ranged from 14 kDa to 31 kDa. This was consistent with the result of the SDS-PAGE analysis. The isoelectric points were found to lie between 3.0 and 8.0, and most of them were in the range of 3.0-6.0. Better separation effect was acquired with pre-prepared immobilized gradient (IPG) strip (pH 3-5.6), and about 320 protein spots could be visualized on the 2-DE map by staining. Within pH 3-l0 and pH 3-5.6 strips, the protein samples of Alexandrium sp. LC3 could be separated well.展开更多
文摘This study on the temporal and spatial variability of the viscosity and some chemical parameters in the sea surface microlayer (SML), the relationship between the viscosity and chemical parameters, and the influence of the viscosity on the mass transfer coefficient ( K ) in the flux of materials through the air sea interface revealed that: The values of viscosity and some chemical parameters in the SML are higher than those in the sub surface layer (SSL), and at daytime are higher than those at night. The viscosity has positive corelation with chemical oxygen demand (COD), dissolved organic carbon (DOC) and salinity. The "SML effect" on K need not be considered because the SML effect on materials concentration is so small.
文摘The fourth-order B spline wavelet scaling functions are used to solve the two-dimensional unsteady diffusion equation. The calculations from a case history indicate that the method provides high accuracy and the computational efficiency is enhanced due to the small matrix derived from this method.The respective features of 3-spline wavelet scaling functions,4-spline wavelet scaling functions and quasi-wavelet used to solve the two-dimensional unsteady diffusion equation are compared. The proposed method has potential applications in many fields including marine science.
基金This research was financially supported by National Natural Science Foundation of China (40206022).
文摘Two-dimensional electrophoresis(2-DE) of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa-31 kDa and 43 kDa-66 kDa, and more proteins were detected between 14kDa and 31 kDa. With the improved protein preparation, the two-dimensional electrophoresis patterns indicated that the relative molecular weights of the proteins were between 14kDa and 100kDa, and most of them ranged from 14 kDa to 31 kDa. This was consistent with the result of the SDS-PAGE analysis. The isoelectric points were found to lie between 3.0 and 8.0, and most of them were in the range of 3.0-6.0. Better separation effect was acquired with pre-prepared immobilized gradient (IPG) strip (pH 3-5.6), and about 320 protein spots could be visualized on the 2-DE map by staining. Within pH 3-l0 and pH 3-5.6 strips, the protein samples of Alexandrium sp. LC3 could be separated well.