The traditional manner to design public transportation system is to sequentially design the transit network and public bicycle network. A new public transportation system design problem that simultaneously considers b...The traditional manner to design public transportation system is to sequentially design the transit network and public bicycle network. A new public transportation system design problem that simultaneously considers both bus network design and public bicycle network design is proposed. The chemical reaction optimization(CRO) is designed to solve the problem. A shortcoming of CRO is that, when the two-molecule collisions take place, the molecules are randomly picked from the container.Hence, we improve CRO by employing different mating strategies. The computational results confirm the benefits of the mating strategies. Numerical experiments are conducted on the Sioux-Falls network. A comparison with the traditional sequential modeling framework indicates that the proposed approach has a better performance and is more robust. The practical applicability of the approach is proved by employing a real size network.展开更多
The Saharan areas and in particular the isolated sites are characterized by low levels of population, in dispersed cores, which suffer from serious problems of water supply for consumption and the irrigation. The phot...The Saharan areas and in particular the isolated sites are characterized by low levels of population, in dispersed cores, which suffer from serious problems of water supply for consumption and the irrigation. The photovoltaic pumping systems （PV） are an ideal solution for these problems. Despite the presence of several photovoltaic pumping systems （PVPS） projects, data acquisition system is still missing in most cases. In this work, the authors will present the PVPS installed at the unit of research in renewable energies in Saharan medium （URERMS, Algeria）. This system will enable us to better analyze the performances ofa photovoltaic pumping system in Saharan medium. The principal elements of the chain of the photovoltaic pumping system are： a photovoltaic generator, the power conditioning system, a motor-driven pump immersed group, an electric wiring, a hydraulic infrastructure which comprises a water tank.展开更多
The development history of the project design of spaceflight launching site in China and the main forms used in these designs were introduced in this thesis, and it was pointed out that the spaceflight launch should n...The development history of the project design of spaceflight launching site in China and the main forms used in these designs were introduced in this thesis, and it was pointed out that the spaceflight launch should not use only one fixed model. According to its nation condition, China should design and build the launching project facility and device which correspond to the technology development of spacecraft and carrier rocket.展开更多
Many websites use verification codes to prevent users from using the machine automatically to register,login,malicious vote or irrigate but it brought great burden to the enterprises involved in internet marketing as ...Many websites use verification codes to prevent users from using the machine automatically to register,login,malicious vote or irrigate but it brought great burden to the enterprises involved in internet marketing as entering the verification code manually.Improving the verification code security system needs the identification method as the corresponding testing system.We propose an anisotropic heat kernel equation group which can generate a heat source scale space during the kernel evolution based on infinite heat source axiom,design a multi-step anisotropic verification code identification algorithm which includes core procedure of building anisotropic heat kernel,settingwave energy information parameters,combing outverification codccharacters and corresponding peripheral procedure of gray scaling,binarizing,denoising,normalizing,segmenting and identifying,give out the detail criterion and parameter set.Actual test show the anisotropic heat kernel identification algorithm can be used on many kinds of verification code including text characters,mathematical,Chinese,voice,3D,programming,video,advertising,it has a higher rate of 25%and 50%than neural network and context matching algorithm separately for Yahoo site,49%and 60%for Captcha site,20%and 52%for Baidu site,60%and 65%for 3DTakers site,40%,and 51%.for MDP site.展开更多
The subway stations of urban community type are not only main nodes in the urban traffic network, but also important places for peopled daily life. Widi the rapid development of urban metro and urban economy, the ...The subway stations of urban community type are not only main nodes in the urban traffic network, but also important places for peopled daily life. Widi the rapid development of urban metro and urban economy, the commercial space near tiie subway station has become the focus of urban planning and urban spatial development, and commercial self-oiganization pkys an important role in the development of commercial operations, commercial structures, and commercial scales, so how to make effective use of the inherent laws of the commercial space evolution, and further guide the rational development of community businesses needs more attention and research. This paper takes Chengdu Wannianchang Subway Station as an example to study the evolution stage, influencing factors, evolution characteristics and laws of the neatby commercial space. Moreover, suggestions were put forward such as coordinating competition and collaboration, optimizing space design and management, co-directional self-organization and heter-oiganizations, in order to provide a new reference for the healthy and ordedy development of the neighboring commefdal space near the subway stations of community type.展开更多
The objective of Performance-Based Earthquake Engineering （PBEE） is the analysis of performance objectives with a specified annual probability of exceedance. Increasingly undesirable performance is caused by increas...The objective of Performance-Based Earthquake Engineering （PBEE） is the analysis of performance objectives with a specified annual probability of exceedance. Increasingly undesirable performance is caused by increasing levels of strong ground motion having decreasing annual probabilities of exceedance. The development of this methodology includes three steps： （1） evaluation of the distribution of ground motion at a site; （2） evaluation of the distribution of system response; （3） evaluation of the probability of exceeding decision variables within a given time period, given appropriate damage measures. The work has taken a systematic approach to determine the impact of increasing levels of detail in site characterization on the accuracy of ground motion and site effects predictions. Complementary studies have investigated the use of the following models for evaluating site effects： （1） amplification factors defined on the basis of generalized site categories, （2） one-dimensional ground response analysis, and （3） two-dimensional ground response analysis for surface topography on ground motion. The paper provides a brief synthesis of ground motion and site effects analysis procedures within a Performance-Based Design framework. It focuses about the influence on the evaluation of site effects in some active regions by different shear waves velocity measurements Down Hole （D-H）, Cross Hole （C-H）, Seismic Dilatometer Marchetti Test （SDMT） and by different variation of shear modulus and damping ratio with strain level and depth from different laboratory dynamic tests for soil characterization： Resonant Column Test （RCT）, Cyclic Loading Torsional Shear Test （CLTST）.展开更多
through the teaching design of online course quality and application practice, the instructional design process based on B/S learning are analyzed, and discusses several key problems in the design and teaching. The pr...through the teaching design of online course quality and application practice, the instructional design process based on B/S learning are analyzed, and discusses several key problems in the design and teaching. The proposed resource-based learning teaching design network course should adapt to the application mode of network teaching and traditional classroom combination; will provide teaching demonstration, teaching content self testing evaluation for students, and to provide jobs for teachers management, online Q ＆ A, BBS, database and knowledge construction and other functions, greatly improving the efficiency of teaching ＂excellent course＂ .展开更多
Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method ...Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and then the amino acid sequence was reversely translated into DNA sequence with degenerate codons, resulting in a large number of sequences with silent mutations, which contained various restriction endonu-clease (RE) sites. Certain mutated sequence with an appropriate RE site was selected as the target DNA sequence for designing a pair of mutation primers to amplify the full-length plasmid via inverse PCR. The amplified product was 5′-phosphorylated, cir-cularized, and transformed into an Escherichia coli host. The transformants were screened by digesting with the designed RE. This protocol uses only one pair of primers and only one PCR is conducted, without the need for hybridization with hazardous isotope for mutant screening or subcloning step.展开更多
基金Projects(71301115,71271150,71101102)supported by the National Natural Science Foundation of ChinaProject(20130032120009)supported by Specialized Research Fund for the Doctoral Program of Higher Education of China
文摘The traditional manner to design public transportation system is to sequentially design the transit network and public bicycle network. A new public transportation system design problem that simultaneously considers both bus network design and public bicycle network design is proposed. The chemical reaction optimization(CRO) is designed to solve the problem. A shortcoming of CRO is that, when the two-molecule collisions take place, the molecules are randomly picked from the container.Hence, we improve CRO by employing different mating strategies. The computational results confirm the benefits of the mating strategies. Numerical experiments are conducted on the Sioux-Falls network. A comparison with the traditional sequential modeling framework indicates that the proposed approach has a better performance and is more robust. The practical applicability of the approach is proved by employing a real size network.
文摘The Saharan areas and in particular the isolated sites are characterized by low levels of population, in dispersed cores, which suffer from serious problems of water supply for consumption and the irrigation. The photovoltaic pumping systems （PV） are an ideal solution for these problems. Despite the presence of several photovoltaic pumping systems （PVPS） projects, data acquisition system is still missing in most cases. In this work, the authors will present the PVPS installed at the unit of research in renewable energies in Saharan medium （URERMS, Algeria）. This system will enable us to better analyze the performances ofa photovoltaic pumping system in Saharan medium. The principal elements of the chain of the photovoltaic pumping system are： a photovoltaic generator, the power conditioning system, a motor-driven pump immersed group, an electric wiring, a hydraulic infrastructure which comprises a water tank.
文摘The development history of the project design of spaceflight launching site in China and the main forms used in these designs were introduced in this thesis, and it was pointed out that the spaceflight launch should not use only one fixed model. According to its nation condition, China should design and build the launching project facility and device which correspond to the technology development of spacecraft and carrier rocket.
基金The national natural science foundation(61273290,61373147)Xiamen Scientific Plan Project(2014S0048,3502Z20123037)+1 种基金Fujian Scientific Plan Project(2013HZ0004-1)FuJian provincial education office A-class project(-JA13238)
文摘Many websites use verification codes to prevent users from using the machine automatically to register,login,malicious vote or irrigate but it brought great burden to the enterprises involved in internet marketing as entering the verification code manually.Improving the verification code security system needs the identification method as the corresponding testing system.We propose an anisotropic heat kernel equation group which can generate a heat source scale space during the kernel evolution based on infinite heat source axiom,design a multi-step anisotropic verification code identification algorithm which includes core procedure of building anisotropic heat kernel,settingwave energy information parameters,combing outverification codccharacters and corresponding peripheral procedure of gray scaling,binarizing,denoising,normalizing,segmenting and identifying,give out the detail criterion and parameter set.Actual test show the anisotropic heat kernel identification algorithm can be used on many kinds of verification code including text characters,mathematical,Chinese,voice,3D,programming,video,advertising,it has a higher rate of 25%and 50%than neural network and context matching algorithm separately for Yahoo site,49%and 60%for Captcha site,20%and 52%for Baidu site,60%and 65%for 3DTakers site,40%,and 51%.for MDP site.
基金Sponsored by Technology Research Program of Sichuan Province of China(2016JY0111)
文摘The subway stations of urban community type are not only main nodes in the urban traffic network, but also important places for peopled daily life. Widi the rapid development of urban metro and urban economy, the commercial space near tiie subway station has become the focus of urban planning and urban spatial development, and commercial self-oiganization pkys an important role in the development of commercial operations, commercial structures, and commercial scales, so how to make effective use of the inherent laws of the commercial space evolution, and further guide the rational development of community businesses needs more attention and research. This paper takes Chengdu Wannianchang Subway Station as an example to study the evolution stage, influencing factors, evolution characteristics and laws of the neatby commercial space. Moreover, suggestions were put forward such as coordinating competition and collaboration, optimizing space design and management, co-directional self-organization and heter-oiganizations, in order to provide a new reference for the healthy and ordedy development of the neighboring commefdal space near the subway stations of community type.
文摘The objective of Performance-Based Earthquake Engineering （PBEE） is the analysis of performance objectives with a specified annual probability of exceedance. Increasingly undesirable performance is caused by increasing levels of strong ground motion having decreasing annual probabilities of exceedance. The development of this methodology includes three steps： （1） evaluation of the distribution of ground motion at a site; （2） evaluation of the distribution of system response; （3） evaluation of the probability of exceeding decision variables within a given time period, given appropriate damage measures. The work has taken a systematic approach to determine the impact of increasing levels of detail in site characterization on the accuracy of ground motion and site effects predictions. Complementary studies have investigated the use of the following models for evaluating site effects： （1） amplification factors defined on the basis of generalized site categories, （2） one-dimensional ground response analysis, and （3） two-dimensional ground response analysis for surface topography on ground motion. The paper provides a brief synthesis of ground motion and site effects analysis procedures within a Performance-Based Design framework. It focuses about the influence on the evaluation of site effects in some active regions by different shear waves velocity measurements Down Hole （D-H）, Cross Hole （C-H）, Seismic Dilatometer Marchetti Test （SDMT） and by different variation of shear modulus and damping ratio with strain level and depth from different laboratory dynamic tests for soil characterization： Resonant Column Test （RCT）, Cyclic Loading Torsional Shear Test （CLTST）.
文摘through the teaching design of online course quality and application practice, the instructional design process based on B/S learning are analyzed, and discusses several key problems in the design and teaching. The proposed resource-based learning teaching design network course should adapt to the application mode of network teaching and traditional classroom combination; will provide teaching demonstration, teaching content self testing evaluation for students, and to provide jobs for teachers management, online Q ＆ A, BBS, database and knowledge construction and other functions, greatly improving the efficiency of teaching ＂excellent course＂ .
基金Project supported by the Hi-Tech Research and Development (863) Program of China (No. 2007AA02Z151)the National Natural Science Foundation of China (No. 30872223)
文摘Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and then the amino acid sequence was reversely translated into DNA sequence with degenerate codons, resulting in a large number of sequences with silent mutations, which contained various restriction endonu-clease (RE) sites. Certain mutated sequence with an appropriate RE site was selected as the target DNA sequence for designing a pair of mutation primers to amplify the full-length plasmid via inverse PCR. The amplified product was 5′-phosphorylated, cir-cularized, and transformed into an Escherichia coli host. The transformants were screened by digesting with the designed RE. This protocol uses only one pair of primers and only one PCR is conducted, without the need for hybridization with hazardous isotope for mutant screening or subcloning step.
