The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make_up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, includi...The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make_up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor_FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber_FISH (EDF_FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3) In situ PCR_based FISH; (4) Bacterial (or yeast) artificial chromosome_FISH (BAC_FISH or YAC_FISH); (5) Tyramide signal amplification_FISH (TSA_FISH); (6) Polypeptide nucleic acid_FISH (PNA_FISH) and (7) padlock_FISH.展开更多
针对眼斑拟石首鱼Sciaenops ocellatus染色体标记匮乏的问题,利用荧光原位杂交(FISH)定位了眼斑拟石首鱼的18S r DNA、5S r DNA和端粒序列。结果显示,眼斑拟石首鱼的核型公式为2n=48t;仅有1对18S r DNA位点,位于第1对染色体的次缢痕部位...针对眼斑拟石首鱼Sciaenops ocellatus染色体标记匮乏的问题,利用荧光原位杂交(FISH)定位了眼斑拟石首鱼的18S r DNA、5S r DNA和端粒序列。结果显示,眼斑拟石首鱼的核型公式为2n=48t;仅有1对18S r DNA位点,位于第1对染色体的次缢痕部位;有2对5S r DNA位点,FISH信号强度不等,强信号位于第8对染色体的近着丝粒端,弱信号位于第3对染色体的臂间。端粒FISH信号出现于所有染色体的两端,但表现出染色体两端信号不平衡的特点,着丝粒端FISH信号明显强于远端信号。这一特点为判定染色体的方向提供了便利。结合其他石首鱼的核型数据可以推断,2n=48t的核型及单对近着丝粒分布的18S r DNA位点是石首鱼的共同祖征;在石首鱼进化过程中,曾发生活跃但不影响宏观核型的小规模重排。研究结果丰富了眼斑拟石首鱼染色体的辨识标记,并为研究石首鱼染色体进化提供了基础数据。展开更多
文摘The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make_up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor_FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber_FISH (EDF_FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3) In situ PCR_based FISH; (4) Bacterial (or yeast) artificial chromosome_FISH (BAC_FISH or YAC_FISH); (5) Tyramide signal amplification_FISH (TSA_FISH); (6) Polypeptide nucleic acid_FISH (PNA_FISH) and (7) padlock_FISH.
文摘针对眼斑拟石首鱼Sciaenops ocellatus染色体标记匮乏的问题,利用荧光原位杂交(FISH)定位了眼斑拟石首鱼的18S r DNA、5S r DNA和端粒序列。结果显示,眼斑拟石首鱼的核型公式为2n=48t;仅有1对18S r DNA位点,位于第1对染色体的次缢痕部位;有2对5S r DNA位点,FISH信号强度不等,强信号位于第8对染色体的近着丝粒端,弱信号位于第3对染色体的臂间。端粒FISH信号出现于所有染色体的两端,但表现出染色体两端信号不平衡的特点,着丝粒端FISH信号明显强于远端信号。这一特点为判定染色体的方向提供了便利。结合其他石首鱼的核型数据可以推断,2n=48t的核型及单对近着丝粒分布的18S r DNA位点是石首鱼的共同祖征;在石首鱼进化过程中,曾发生活跃但不影响宏观核型的小规模重排。研究结果丰富了眼斑拟石首鱼染色体的辨识标记,并为研究石首鱼染色体进化提供了基础数据。
