Objective:To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutin...Objective:To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains of Leptospira interrongans belonging to 15 serogroups and 2 strains of Leptospira biflexa belonging to 2 serogroups.The outer envelopes (OEs) of L.interrogans serogroup Icterohaemorrhagiae serovar lai strain lai (56601) with strong virulence and serogroup Pomona serovar pomona strain Luo (56608) with low virulence,and L.biflexa serogroup Semaranga serovar patoc strain Patoc I without virulence were prepared by using the method reported in Auran et al.(1972).OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively. Results:All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256-1:512. A similar SDS-PAGE pattern of the OMPs from the three strains of leptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 KDa.A positive protein fragment with approximately 32 KDa confirmed by Western blot,was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains of leptospira.Conclusion: There are genus-specific antigens on the surface of L.interrogans and L.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens of leptospira.展开更多
To obtain an accurate and robust soft sensor model in dealing with the increasingly complex industrial modeling data,an effective heterogeneous ensemble of extreme learning machines(HEELM)is proposed.Specifically,the ...To obtain an accurate and robust soft sensor model in dealing with the increasingly complex industrial modeling data,an effective heterogeneous ensemble of extreme learning machines(HEELM)is proposed.Specifically,the kernel extreme learning machine(KELM)and four common extreme learning machine(ELM)models that have different internal activations are contained in the HEELM for enriching the diversity of sub-models.The number of hidden layer nodes of the extreme learning machine is determined by the trial and error method,and the optimal parameters of the kernel extreme learning machine model are determined by cross validation.Moreover,to obtain the best output of the ensemble model,least squares regression is applied to aggregate the outputs of all individual models.Two complex data sets of practical industrial processes are used to test the HEELM performance.The simulation results show that the HEELM has a high prediction accuracy.Compared with the individual ELM models,bagging ELM ensemble model,BP and SVM models,the prediction accuracy of the HEELM model is improved by 4.5%to 8.7%,and the HEELM model can obtain better generalization capability.展开更多
This paper presents an analysis of the impact of wind on the transport of the Changiiang River Diluted Water （CRDW） in August by using the salinity data col- lected on two zonal sections near Cheju-do. Based on the ...This paper presents an analysis of the impact of wind on the transport of the Changiiang River Diluted Water （CRDW） in August by using the salinity data col- lected on two zonal sections near Cheju-do. Based on the climatological mean conditions and four extreme events, the analysis indicates that wind-induced Ekman transport plays an important role in the extension of the CRDW. The strong northeastward Ekman transport induced by southeasterly wind in 1996, 2003, 2004, and 2006 pushes the core of the CRDW to the sea adjacent to Cheju-do. A comparison of the wind variation before observation among these four extreme events indicates that the expan- sion pattern of the CRDW is primarily changed by synop- tic variation with tirnescales of days to weeks, such as during a typhoon. The weak eastward extension of the CRDW in 2004, accompanied with a relatively strong southerly wind, implies that the oceanographic state （e.g., the depth of halocline） may strongly affect the impact of wind on the extension of the CRDW.展开更多
Climate change will impact agriculture and food production around the world due to the effects of elevated CO2 in the atmosphere, higher temperature, altered precipitation and transpiration regimes, increased frequenc...Climate change will impact agriculture and food production around the world due to the effects of elevated CO2 in the atmosphere, higher temperature, altered precipitation and transpiration regimes, increased frequency of extreme events, and modified weed, pest and pathogen pressure. Data of this study were collected from primary and secondary sources. Primary sources of data were collected using two sets of structured questionnaires. A total number of three hundred （300） questionnaires were administered on categories of respondents in this study. Results of this study revealed that food crops are the major crops cultivated in the study area, and the effects of climate change on agricultural production results in soil loss, plant nutrient loss, textural change, increase in pest and diseases and poor yield germination etc.. This study therefore recommended that cover trees should be planted, which will provide shade and reduce heat, due to climate change and the preservation of underground water.展开更多
文摘Objective:To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains of Leptospira interrongans belonging to 15 serogroups and 2 strains of Leptospira biflexa belonging to 2 serogroups.The outer envelopes (OEs) of L.interrogans serogroup Icterohaemorrhagiae serovar lai strain lai (56601) with strong virulence and serogroup Pomona serovar pomona strain Luo (56608) with low virulence,and L.biflexa serogroup Semaranga serovar patoc strain Patoc I without virulence were prepared by using the method reported in Auran et al.(1972).OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively. Results:All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256-1:512. A similar SDS-PAGE pattern of the OMPs from the three strains of leptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 KDa.A positive protein fragment with approximately 32 KDa confirmed by Western blot,was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains of leptospira.Conclusion: There are genus-specific antigens on the surface of L.interrogans and L.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens of leptospira.
基金The National Natural Science Foundation of China(No.71471060)the Natural Science Foundation of Hebei Province(No.E2018502111)Fundamental Research Funds for the Central Universities(No.2019QN134).
文摘To obtain an accurate and robust soft sensor model in dealing with the increasingly complex industrial modeling data,an effective heterogeneous ensemble of extreme learning machines(HEELM)is proposed.Specifically,the kernel extreme learning machine(KELM)and four common extreme learning machine(ELM)models that have different internal activations are contained in the HEELM for enriching the diversity of sub-models.The number of hidden layer nodes of the extreme learning machine is determined by the trial and error method,and the optimal parameters of the kernel extreme learning machine model are determined by cross validation.Moreover,to obtain the best output of the ensemble model,least squares regression is applied to aggregate the outputs of all individual models.Two complex data sets of practical industrial processes are used to test the HEELM performance.The simulation results show that the HEELM has a high prediction accuracy.Compared with the individual ELM models,bagging ELM ensemble model,BP and SVM models,the prediction accuracy of the HEELM model is improved by 4.5%to 8.7%,and the HEELM model can obtain better generalization capability.
基金supported by the National Natural Science Foundation of China (40906014 and 40976015)the Marine Science Foundation of State Oceanic Administration of China for the Youth (2010218)
文摘This paper presents an analysis of the impact of wind on the transport of the Changiiang River Diluted Water （CRDW） in August by using the salinity data col- lected on two zonal sections near Cheju-do. Based on the climatological mean conditions and four extreme events, the analysis indicates that wind-induced Ekman transport plays an important role in the extension of the CRDW. The strong northeastward Ekman transport induced by southeasterly wind in 1996, 2003, 2004, and 2006 pushes the core of the CRDW to the sea adjacent to Cheju-do. A comparison of the wind variation before observation among these four extreme events indicates that the expan- sion pattern of the CRDW is primarily changed by synop- tic variation with tirnescales of days to weeks, such as during a typhoon. The weak eastward extension of the CRDW in 2004, accompanied with a relatively strong southerly wind, implies that the oceanographic state （e.g., the depth of halocline） may strongly affect the impact of wind on the extension of the CRDW.
文摘Climate change will impact agriculture and food production around the world due to the effects of elevated CO2 in the atmosphere, higher temperature, altered precipitation and transpiration regimes, increased frequency of extreme events, and modified weed, pest and pathogen pressure. Data of this study were collected from primary and secondary sources. Primary sources of data were collected using two sets of structured questionnaires. A total number of three hundred （300） questionnaires were administered on categories of respondents in this study. Results of this study revealed that food crops are the major crops cultivated in the study area, and the effects of climate change on agricultural production results in soil loss, plant nutrient loss, textural change, increase in pest and diseases and poor yield germination etc.. This study therefore recommended that cover trees should be planted, which will provide shade and reduce heat, due to climate change and the preservation of underground water.
