采用短短芽孢杆菌(BB)、竹浆携带微生物区系(HM)、BB+HM(HBB)3种微生物策略降解竹纤维素纤维(BCF)。通过摇瓶发酵实验探究不同微生物策略的产酶和降解功能。借助黏度法、XRD、FTIR和16S r RNA测序技术分析最佳处理组底物结构和细菌群落...采用短短芽孢杆菌(BB)、竹浆携带微生物区系(HM)、BB+HM(HBB)3种微生物策略降解竹纤维素纤维(BCF)。通过摇瓶发酵实验探究不同微生物策略的产酶和降解功能。借助黏度法、XRD、FTIR和16S r RNA测序技术分析最佳处理组底物结构和细菌群落组成。结果表明:在降解BCF的过程中,HBB表现出最高的主要降解期羧甲基纤维素钠酶活性、漆酶活性和丰富的木聚糖酶活性;也观察到BCF纤维尺寸迅速减小、碳水化合物和木质素连接键(LCC)破坏及去除,结晶区缺陷等结构特征的改变;发酵结束时,HBB降解体系的干物质损失达到77.67%。HBB细菌微生物涵盖了18门,26纲,55目,85科,143属,在属水平上,主要降解期和降解后期菌属物种差异较大。德沃斯氏菌属、苍白杆菌属、Taibaiella、短波单孢菌属在主要降解期更加丰富,而假黄单孢菌属、科恩氏菌属、未分类黄杆菌目在降解后期更高。PCoA分析和Anosim分析也表明,降解过程不同时期样本细菌群落结构具有显著性差异。HBB能加速BCF的降解,其中短芽孢杆菌属、Devosia、Ochrobactrum、Taibaiella、Pseudoxanthomona、固氮螺菌属、纤维单孢菌属是BCF高效降解的优势菌属。展开更多
Two direct dyes were applied to conventional viscose(CV)and bamboo viscose(BV)fibers,which were prepared from bamboo cellulose pulps,and the dyeing kinetics of two fibers were compared.Three kinetic equations,namely C...Two direct dyes were applied to conventional viscose(CV)and bamboo viscose(BV)fibers,which were prepared from bamboo cellulose pulps,and the dyeing kinetics of two fibers were compared.Three kinetic equations,namely Chrastil,Cegarra-Puente,and Vickerstaff,were used to fit the experimental dyeing rate points,showing that the best result was obtained by the Chrastil equation.BV fibers displayed slightly higher dyeing rates and dye adsorption values at initial stages,but a bit lower dye adsorption values at equilibrium than CV fibers.Furthermore,the dyeing of BV fibers exhibited lower activation energies and higher dyeing rate constants than that of CV fibers,and therefore showed slightly lower dependence on temperature.展开更多
文摘采用短短芽孢杆菌(BB)、竹浆携带微生物区系(HM)、BB+HM(HBB)3种微生物策略降解竹纤维素纤维(BCF)。通过摇瓶发酵实验探究不同微生物策略的产酶和降解功能。借助黏度法、XRD、FTIR和16S r RNA测序技术分析最佳处理组底物结构和细菌群落组成。结果表明:在降解BCF的过程中,HBB表现出最高的主要降解期羧甲基纤维素钠酶活性、漆酶活性和丰富的木聚糖酶活性;也观察到BCF纤维尺寸迅速减小、碳水化合物和木质素连接键(LCC)破坏及去除,结晶区缺陷等结构特征的改变;发酵结束时,HBB降解体系的干物质损失达到77.67%。HBB细菌微生物涵盖了18门,26纲,55目,85科,143属,在属水平上,主要降解期和降解后期菌属物种差异较大。德沃斯氏菌属、苍白杆菌属、Taibaiella、短波单孢菌属在主要降解期更加丰富,而假黄单孢菌属、科恩氏菌属、未分类黄杆菌目在降解后期更高。PCoA分析和Anosim分析也表明,降解过程不同时期样本细菌群落结构具有显著性差异。HBB能加速BCF的降解,其中短芽孢杆菌属、Devosia、Ochrobactrum、Taibaiella、Pseudoxanthomona、固氮螺菌属、纤维单孢菌属是BCF高效降解的优势菌属。
基金National Key Technology R&D Program of the Chinese Ministry of Science and Technology,China(No.2007BAE41B04)
文摘Two direct dyes were applied to conventional viscose(CV)and bamboo viscose(BV)fibers,which were prepared from bamboo cellulose pulps,and the dyeing kinetics of two fibers were compared.Three kinetic equations,namely Chrastil,Cegarra-Puente,and Vickerstaff,were used to fit the experimental dyeing rate points,showing that the best result was obtained by the Chrastil equation.BV fibers displayed slightly higher dyeing rates and dye adsorption values at initial stages,but a bit lower dye adsorption values at equilibrium than CV fibers.Furthermore,the dyeing of BV fibers exhibited lower activation energies and higher dyeing rate constants than that of CV fibers,and therefore showed slightly lower dependence on temperature.
