Between March and May 2005, bird communities in four sample plots at Baita Airport were studied using strips methods in Hohhot, Inner Mongolia. A total of 59 species belonging to 10 orders and 26 families were recorde...Between March and May 2005, bird communities in four sample plots at Baita Airport were studied using strips methods in Hohhot, Inner Mongolia. A total of 59 species belonging to 10 orders and 26 families were recorded. Based on the principles of avian community ecology, the community parameters were discussed, including the comparative importance value, distribution coefficient, density and probability of interactive encounter. By analyzing these indices, combined with their flight behaviors at the airport and its neighbourhood, we identified bird species that have the potential to threaten flight security at Baita Airport; The results showed that the 23 bird species including magpie and red falcon are the most hazard to flight security and the eight bird species including sparrow Hawk and Greenfinch are the hazard. Furthermore, we assessed the bird species＇ different hazard grades to flight security.展开更多
A real-time monitoring reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the sensitive and specific detection of prototypic,prevalent North American porcine reproductive an...A real-time monitoring reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the sensitive and specific detection of prototypic,prevalent North American porcine reproductive and respiratory syndrome virus (PRRSV) strains.As a higher sensitivity and specificity method than reverse transcription polymerase chain reaction (RT-PCR),the RT-LAMP method only used a turbidimeter,exhibited a detection limit corresponding to a 10-4 dilution of template RNA extracted from 250 μL of 105 of the 50% tissue culture infective dose (TCID50) of PRRSV-containing cells,and no cross-reactivity was observed with other related viruses including porcine circovirus type 2,swine influenza virus,porcine rotavirus and classical swine fever virus.From forty-two field samples,33 samples in the RT-LAMP assay was detected positive,whereas three of which were not detected by RT-PCR.Furthermore,in 33 strains of PRRSV,an identical detection rate was observed with the RT-LAMP assay to what were isolated using porcine alveolar macrophages.These findings demonstrated that the RT-LAMP assay has potential clinical applications for the detection of highly pathogenic PRRSV isolates,especially in developing countries.展开更多
SUPER DOLLFIE——这个身穿蕾丝花边公主裙、卷发碧眼的"超级娃娃"是由日本设计师圆句昭浩开发塑造、VOLKS公司生产的1/3球型关节可动人偶。常见的SD为58cm,迷你SD高43cm,而最新推出的SD13则为60cm。其价格也不划一,正版的娃...SUPER DOLLFIE——这个身穿蕾丝花边公主裙、卷发碧眼的"超级娃娃"是由日本设计师圆句昭浩开发塑造、VOLKS公司生产的1/3球型关节可动人偶。常见的SD为58cm,迷你SD高43cm,而最新推出的SD13则为60cm。其价格也不划一,正版的娃娃通常介乎于人民币4500～8500元左右,迷你的也在2000～4000元之间,真真切切的"贵"族!由于门槛高,所以玩家以童心未泯、又有经济基础的N世代新贵为主。展开更多
文摘Between March and May 2005, bird communities in four sample plots at Baita Airport were studied using strips methods in Hohhot, Inner Mongolia. A total of 59 species belonging to 10 orders and 26 families were recorded. Based on the principles of avian community ecology, the community parameters were discussed, including the comparative importance value, distribution coefficient, density and probability of interactive encounter. By analyzing these indices, combined with their flight behaviors at the airport and its neighbourhood, we identified bird species that have the potential to threaten flight security at Baita Airport; The results showed that the 23 bird species including magpie and red falcon are the most hazard to flight security and the eight bird species including sparrow Hawk and Greenfinch are the hazard. Furthermore, we assessed the bird species＇ different hazard grades to flight security.
文摘A real-time monitoring reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the sensitive and specific detection of prototypic,prevalent North American porcine reproductive and respiratory syndrome virus (PRRSV) strains.As a higher sensitivity and specificity method than reverse transcription polymerase chain reaction (RT-PCR),the RT-LAMP method only used a turbidimeter,exhibited a detection limit corresponding to a 10-4 dilution of template RNA extracted from 250 μL of 105 of the 50% tissue culture infective dose (TCID50) of PRRSV-containing cells,and no cross-reactivity was observed with other related viruses including porcine circovirus type 2,swine influenza virus,porcine rotavirus and classical swine fever virus.From forty-two field samples,33 samples in the RT-LAMP assay was detected positive,whereas three of which were not detected by RT-PCR.Furthermore,in 33 strains of PRRSV,an identical detection rate was observed with the RT-LAMP assay to what were isolated using porcine alveolar macrophages.These findings demonstrated that the RT-LAMP assay has potential clinical applications for the detection of highly pathogenic PRRSV isolates,especially in developing countries.
