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K等核系列离子3d电子轨道波函数性态研究 被引量:1
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作者 李继弘 《宁夏师范学院学报》 2008年第3期25-29,共5页
利用HFR自洽场方法计算了K等核系列离子3d,4d电子径向波函数,分析了沿K等核系列离子3d轨道波函数的塌缩现象,并进一步讨论了轨道波函数的控制塌缩.
关键词 等核 离子 3d波函数
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Ar等核系列离子3d电子轨道波函数性态研究
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作者 李继弘 《周口师范学院学报》 CAS 2008年第2期55-57,79,共4页
利用HFR自洽场方法计算了Ar等核系列离子3d,4d电子径向波函数,分析了沿Ar等核系列离子3d轨道波函数的塌缩现象,并进一步讨论了轨道波函数的控制塌缩.
关键词 等核系列 离子 3d波函数
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论集团公司预算管理和绩效考核构建
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作者 王刚 《中国经贸》 2011年第16期203-203,共1页
本文分别从集团公司的预算管理和绩效考核体系的建立两大块出发,对完善集团公司的预算管理和绩效考核体系提出了一些看法和建议。
关键词 集团公司 预算管理 绩效 等核体系
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四元数体上矩阵的酉性与矩阵分解
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作者 冯良贵 《自然科学进展》 北大核心 2006年第11期1472-1477,共6页
给出了四元数酉阵右特征向量集的一个数值特征,剖析了四元数部分酉阵的本质结构.利用四元数部分酉阵建立了四元数长方阵的等核极分解的存在惟一性定理,进而得到了四元数正规阵的一个新的等价描述,并给出了四元数方阵有惟一极分解的特... 给出了四元数酉阵右特征向量集的一个数值特征,剖析了四元数部分酉阵的本质结构.利用四元数部分酉阵建立了四元数长方阵的等核极分解的存在惟一性定理,进而得到了四元数正规阵的一个新的等价描述,并给出了四元数方阵有惟一极分解的特征刻画.最后,就文中建立的四元数长方阵的等核极分解,给出了实现它的一个具体方法. 展开更多
关键词 四元数酉阵 等核极分解 部分酉阵
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弱幂等Quantale范畴与相关范畴的关系
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作者 刘敏 杜佳慧 《吉林大学学报(理学版)》 CAS 北大核心 2022年第2期277-283,共7页
首先引入弱幂等Quantale及Quantale上弱幂等核映射的概念,给出Quantale的最大弱幂等商的等价刻画;然后证明弱幂等Quantale范畴是Quantale范畴的反射子范畴,幂等Quantale范畴是弱幂等Quantale范畴的反射子范畴;最后得到幂等Quantale范畴... 首先引入弱幂等Quantale及Quantale上弱幂等核映射的概念,给出Quantale的最大弱幂等商的等价刻画;然后证明弱幂等Quantale范畴是Quantale范畴的反射子范畴,幂等Quantale范畴是弱幂等Quantale范畴的反射子范畴;最后得到幂等Quantale范畴是Quantale范畴的反射子范畴. 展开更多
关键词 Quantale商 弱幂等Quantale 弱幂等核映射 范畴
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Directional Breeding of An Oval-ecotype Male Sterile Line of Chinese Cabbage(Brassica campestris L. ssp. pekinensis) 被引量:4
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作者 谭翀 郭瑛琪 岳艳玲 《Agricultural Science & Technology》 CAS 2014年第3期333-337,共5页
[Objective] The study aimed to explore the method for directional breeding of a male-sterile line in oval-ecotype Chinese cabbage. [Method] Based on "Multiple Allele Hypothesis of Genic Male Sterile Chinese Cabbage"... [Objective] The study aimed to explore the method for directional breeding of a male-sterile line in oval-ecotype Chinese cabbage. [Method] Based on "Multiple Allele Hypothesis of Genic Male Sterile Chinese Cabbage", an inbred line '06048' of oval ecotype was used as the receptor, and male fertile plant of 'AB12' was used as the donor line. Crossing, backcross, selfing, testcross and sibling were ap- plied to transfer the multiple alleles under the directional genetic model. [Result] Segregation ratio of every generation was consistent with theoretical value. A new male sterile line with 100% male sterility and '06048' horticultural traits was ob- tained successfully, which accomplished the transfer of male sterile multiple allele and horticultural characters of receptor line at the same time. [Conclusion] The re- search verifies that the model of directional transfer is feasible, provides a theoreti- cal basis for the directional transfer of Chinese cabbage with other horticultural traits whose genotype is msms. The model can also be applied to other Brassica crops, to generate genetic male sterile lines with specific botanical traits and high-quality economic traits. 展开更多
关键词 Chinese cabbage Multiple-allele genic male sterility Directional transfer
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Study on Adsorption of Hg(Ⅱ) by Chinese Walnut(Juglans mandshurica Maxim.) of Biomass Material 被引量:3
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作者 于春光 杜茂安 查金苗 《Agricultural Science & Technology》 CAS 2011年第12期1869-1872,共4页
[Objective] The aim of the study was to make research on adsorption of Chinese walnut(Juglans mandshurica Maxim.) Shell(CWS) to Hg(Ⅱ) in water.[Method] Shells of Juglans mandshurica Maxim were used as biosorpti... [Objective] The aim of the study was to make research on adsorption of Chinese walnut(Juglans mandshurica Maxim.) Shell(CWS) to Hg(Ⅱ) in water.[Method] Shells of Juglans mandshurica Maxim were used as biosorption to remove Hg(Ⅱ) in water solution to explore the influence to adsorption of Hg(Ⅱ) under different conditions,like pH solution,adsorption time,and Hg(Ⅱ).[Result] The experimental results show that when absorptivity of Hg(Ⅱ) by CWS reached the highest,pH ranged within 5.0-6.0.The adsorptivity decreased as initial Hg(Ⅱ) concentrations increased.Fourier Transform Infrared Spectroscopy(FTIR) spectrum revealed some chemical groups of CWS may affect the adsorption of Hg(Ⅱ),such as hydroxyl groups,methyl groups,aromatic methoxyl groups,unconjugated carbonyl,and typical aromatic ring,etc.Adsorption equation can be concluded considering the biosorption process relationship with Langmuir and Frendrich isotherm.[Conclusion] The study found that CW could be employed as a promising biosorption to remove Hg(Ⅱ) from aqueous environments. 展开更多
关键词 BIOSORPTION Chinese walnut shell Hg(Ⅱ)ions Aqueous solution ISOTHERM
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Progress in Research on Intercellular Movement of Protoplasm in Higher Plants 被引量:3
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作者 张伟成 《Acta Botanica Sinica》 CSCD 2002年第9期1068-1074,共7页
Since nuclear extrusion was rediscovered in young Allium scale by S.H.WU in the years of 1950's systematic investigations on this phenomenon were carried out with various kinds of microscopic techniques a... Since nuclear extrusion was rediscovered in young Allium scale by S.H.WU in the years of 1950's systematic investigations on this phenomenon were carried out with various kinds of microscopic techniques and plant materials to collect more effective evidence to clarify the debate about whether the nuclear extrusion is an artifact or normal event. In the cooperative research of S. H. WU and C. H. LOU the normality of the occurrence of nuclear extrusion either in growing part of plant or in senescent tissue has been confirmed. This event is intimately associated with the physiological state of the tissues/cells and may play an important role in redistribution and reutilization of cell contents. Based on the results obtained a hypothesis of intercellular movement of protoplasm as a means of translocation of organic material in plants was suggested. Chromatin extrusion was also discovered in the pollen mother cells (cytomixis) of certain angiosperms by G. C. ZHENG and his team. Intercellular migration of chromatin appears most frequently at the stage of synizesis. Cytomixis has been studied in relation to variation and evolution. Chromosome aberration has been considered to be closely associated with chromatin extrusion. By vital microscopic observations of the live tissues of garlic (Allium sativum L.) bud and wheat (Triticum aestivum L.) ovule combined with cinemicroscopy and video recording it has been uncovered that, not only the nuclear material but also the cytoplasm could traverse the intercellular channels by vigorous contraction and expansion, and they may simultaneously extrude out of a cell but often asynchronously migrate from one cell to another. The involvement of cytoplasmic constituents in intercellular migration was also detected in pollen mother cells with electron microscopy. Regarding the mechanism of intercellular movement a series of experiments provide convincing evidence showing that this kind of movement is an active metabolic process closely coupled with energy metabolism, and the motive power for driving the extrusion may be supplied by the contractile proteins in protoplasm. 展开更多
关键词 nuclear extrusion PLASMODESMA
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Detection of Listeria monocytogenes in Dairy Food by Loop-mediated Isothermal Amplification(LAMP) 被引量:2
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作者 程潇 汪永信 +4 位作者 刘娟娟 张波 徐晨 贾贞 安虹 《Agricultural Science & Technology》 CAS 2015年第8期1584-1587,1599,共5页
[Objective] This study aimed to detect the reliability of LAMP method for detecting Listeria monocytogenes in dairy food. [Method] Based on the sequence of hlyA gene encoding listeriolysin O in Listeria monocytogenes,... [Objective] This study aimed to detect the reliability of LAMP method for detecting Listeria monocytogenes in dairy food. [Method] Based on the sequence of hlyA gene encoding listeriolysin O in Listeria monocytogenes, the LAMP method was established for detecting Listeria monocytogenes in dairy food. [Result] The es- tablished LAMP rapid detection method has .high specificity and sensitivity, which are equivalent to those of real-time fluorescent quantitative PCR. The detection re- sults of Listeria monocytogenes in dairy food by established LAMP method were completely consistent with those by bacterial isolation method. [Conclusion] The de- tection results of Listeria monocytogenes by LAMP method can be directly identified by naked eye, so the established LAMP method was suitable for the detection of Listeria monocytogenes in dairy food in emergency situations. 展开更多
关键词 Listeria monocytogenes hlyA gene Loop-mediated isothermal amplifica-tion
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Microsatellites underestimate genetic divergence in the Green-backed Tit (Parus monticolus) 被引量:2
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作者 王文娟 戴传银 +3 位作者 Bailey D.MCKAY 赵娜 李寿先 雷富民 《Chinese Birds》 CSCD 2013年第2期144-154,共11页
Although a growing number of both sequence-based and microsatellite nuclear loci have been used to infer genetic structures, their relative efficiencies remain poorly understood. In our study, we used the Green-backed... Although a growing number of both sequence-based and microsatellite nuclear loci have been used to infer genetic structures, their relative efficiencies remain poorly understood. In our study, we used the Green-backed Tit (Parus monticolus) to explore the resolving ability of these two types of markers. The south-western and central mitochondrial DNA (mtDNA) phylogroups were divergent to some extent in sequence-based nuclear data, while mixed together in microsatellites data. The F ST values among clades were about four times lower in microsatellite loci than those in sequence-based nuclear loci. We are of the opinion that size homoplasy may have contributed to the inability of microsatellites to uncover differentiation. Our results suggest that sequence-based nuclear loci outperformed microsatellite loci in detecting population structures, especially those focused on populations with large effective population sizes. There was no significant correlation between F ST values and allelic size variability, which suggested that the efficiency of microsatellite loci in detecting genetic structure may be independent of their polymorphism. F ST is better than R ST in detecting intraspecific divergence due to the high variance of R ST . In agreement with sequence-based nuclear loci, microsatellite loci did resolve the genetic distinctness of the Taiwan Residents phylogroup. The genetic differentiation between the Taiwan Residents and continental clades may involve allopatric divergence without gene flow. 展开更多
关键词 genetic structure MICROSATELLITE Parus monticolus sequence-based nuclear data size homoplasy
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PLASMA RESISTIN LEVELS AND SINGLE-NUCLEOTIDE POLYMORPHISMS IN RESISTIN GENE 5 FLANKING REGION IN PATIENTS WITH STROKE 被引量:3
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作者 Xing-jian Lin Ying-dong Zhang Qing-shan Guan Qing Di Jing-ping Shi Wei-guo Liu 《Chinese Medical Sciences Journal》 CAS CSCD 2007年第1期27-32,共6页
Objective To analyze the role of resistin in insulin resistance (IR) through investigating the variation of plasma resistin levels and single-nucleotide polymorphisms (SNPs) in resistin gene 5’ flanking region in str... Objective To analyze the role of resistin in insulin resistance (IR) through investigating the variation of plasma resistin levels and single-nucleotide polymorphisms (SNPs) in resistin gene 5’ flanking region in stroke patients.Methods In 103 atherothrombotic cerebral infarction (ACI) patients, 85 lacunar infarction (LI) patients, 70 intracerebral hemorrhage (ICH) patients, and 86 healthy controls, plasma resistin and insulin levels were measured by ELISA , SNPs in resistin gene 5’ flanking region were detected by PCR and direct DNA sequencing. The subjects’ body height and weight, the body mass index, quantitative insulin sensitivity check index (QUICKI), blood pressure, and the concentration of fasting plasma glucose, triglyceride, total cholesterol, creatinine, low-density lipoprotein, and high-density lipoprotein were also determined. Results QUICKI was significantly lower in the ACI and ICH patients (0.316±0.037 and 0.309±0.032, respectively) than that in the controls (0.342±0.043, P<0.001), while plasma resistin level was significantly higher in the ACI and ICH patients (6.36±3.79 and 7.15±4.27 ng/mL, respectively) than that in the controls (5.28±2.56 ng/mL, P<0.05), but such difference was not observed in the LI patients compared with controls. There was a statistically negative correlation between plasma resistin level with QUICKI (r=-0.228, P<0.001). The distributions of allele and genotype frequencies of resistin gene -420C>G and -537A>C SNPs were not significantly different among the different groups, and those SNPs were not correlated with other clinical and biochemical parameters.Conclusions Plasma resistin is associated with stroke by participating in the development of IR. The SNPs in resistin gene 5’ flanking region has no impact on the plasma resistin level. 展开更多
关键词 RESISTIN insulin resistance single-nucleotide polymorphisms STROKE
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One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA 被引量:9
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作者 Xue-en FANG Jian LI Qin CHEN 《Virologica Sinica》 SCIE CAS CSCD 2008年第3期167-172,共6页
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of fo... Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article. 展开更多
关键词 Nucleic acid amplification Loop-mediated isothermal amplification (LAMP) APPLICATION
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Preliminary Study on the Characteristics of Isotactic Polypropylene with Nucleating Agent Swollen by Supercritical Carbon Dioxide 被引量:1
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作者 李斌 曹贵平 +4 位作者 刘颋 刘涛 赵玲 袁渭康 胡国华 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2005年第5期673-677,共5页
Impregnation of isotactic polypropylene (iPP) with nucleating agent (NA21) using supercritical carbon dioxide as the swelling agent at different temperature and pressure and its non-isothermal crystallization kinetics... Impregnation of isotactic polypropylene (iPP) with nucleating agent (NA21) using supercritical carbon dioxide as the swelling agent at different temperature and pressure and its non-isothermal crystallization kinetics were investigated. The results showed that NA21 was dispersed at a nanometer-scale in the PP matrix, resulting in the formation of different typesof crystal phases of iPP and the enhancement of its mechanical properties. 展开更多
关键词 supercritical carbon dioxide POLYPROPYLENE nucleating agent mechanical performance
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Influence of a nucleotide oligomerization domain 1 (NOD1) polymorphism and NOD2 mutant alleles on Crohn's disease phenotype 被引量:2
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作者 Elisabet Cantó Elena Ricart +6 位作者 David Busquets David Monfort Esther García-Planella Dolors González Joaquim Balanzó José L Rodríguez-Sánchez Sílvia Vidal 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第41期5446-5453,共8页
AIM: To examine genetic variation of nucleotide oligomerization domain 1 (NOD/) and NOD2, their respective influences on Crohn's disease phenotype and gene-gene interactions. METHODS: (ND1+32656*1) NOD1 polym... AIM: To examine genetic variation of nucleotide oligomerization domain 1 (NOD/) and NOD2, their respective influences on Crohn's disease phenotype and gene-gene interactions. METHODS: (ND1+32656*1) NOD1 polymorphism and SNPS, SNP12 and SNP13 of NOD2 were analyzed in 97 patients and 50 controls. NOD2 variants were determined by reaction restriction fragment length polymorphism analysis. NOD1 genotyping and NOD2 variant confirmation were performed by specific amplification and sequencing. RESULTS: The distribution of NOD1 polymorphism in patients was different from controls (P = 0.045) and not altered by existence of NOD2 mutations. In this cohort, 30.92% patients and 6% controls carried at least one NOD2 variant (P 〈 0.001) with R702W being the most frequent variant. Presence of at least one NOD2 mutation was inversely associated with colon involvement (9.09% with colon vs 36.4% with ileal or ileocolonic involvement, P = 0.04) and indicative of risk of penetrating disease (52.63% with penetrating vs 25.64% with non-penetrating or stricturing behavior, P = 0.02). L1007finsC and double NOD2 mutation conferred the highest risk for severity of disease (26.3% with penetrating disease vs 3.8% with non-penetrating or stricturing behavior presented L1007finsC, P = 0.01 and 21.0% with penetrating disease vs 2.5% with non-penentrating or stricturing behavior carried double NOD2 mutation, P = 0.007). Exclusion of patients with NOD2 mutations from phenotype/NODl-genotype analysis revealed higher prevalence of *1*1 genotype in groups of younger age at onset and colonic location.CONCLUSION: This study suggests population differences in the inheritance of risk NOD1 polymorphism and NOD2 mutations. Although no interaction between NOD1-NOD2 was noticed, a relationship between disease location and Nod-like receptor molecules was established. 展开更多
关键词 Crohn's disease Nucleotide oligomerization domain 1 Nucleotide oligomerization domain 2
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Multidrug resistance protein 3 R652G may reduce susceptibility to idiopathic infant cholestasis 被引量:3
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作者 xiu-Qi Chen Lin-Lin Wang Qing-Wen Shan Qing Tang Shu-Jun Lian 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第46期5855-5858,共4页
AIM:To evaluate the role of genetic factors in the pathogenesis of idiopathic infant cholestasis.METHODS:We performed a case-control study,in-cluding 78 infants with idiopathic infant cholestasis and 113 healthy infan... AIM:To evaluate the role of genetic factors in the pathogenesis of idiopathic infant cholestasis.METHODS:We performed a case-control study,in-cluding 78 infants with idiopathic infant cholestasis and 113 healthy infants as controls.Genomic DNA was extracted from peripheral venous blood leukocytes us-ing phenol chloroform methodology.Polymerase chain reaction was used to amplify the multidrug resistance protein 3(MDR3)R652G fragment,and products were sequenced using the ABI 3100 Sequencer.RESULTS:The R652G single nucleotide polymorphism(SNP)was significantly more frequent in healthy infants(allele frequency 8.0%)than in patients(allele frequency 2.60%)(P < 0.05),odds ratio,0.29;95% confidence interval,0.12-0.84.The conjugated bilirubin in patients with the AG genotype was significantly lower than in those with the AA genotype(44.70 ± 6.15 μmol/L vs 95.52 ± 5.93 μmol/L,P < 0.05).CONCLUSION:MDR3 R652G is negatively correlated with idiopathic infant cholestasis.Children with the R652G SNP in Guangxi of China may have reduced susceptibility to infant intrahepatic cholestasis. 展开更多
关键词 Multidrug resistance protein 3 Singlenucleotide polymorphisms R652G INFANT CHOLESTASIS
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Oscillator Strengths for 2s~2-2p~2P Transitions of the Lithium IsoelectronicSequence from NaIX to CaXVIII
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作者 CHENChao WANGZhi-Wen 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第2期305-308,共4页
The nonrelativistic dipole-length, -velocity and -acceleration absorptionoscillator strengths for the 1s~22s-1s~22p transitions of the lithium isoelectronic sequence from Z= 11 to 20 are calculated by using the energi... The nonrelativistic dipole-length, -velocity and -acceleration absorptionoscillator strengths for the 1s~22s-1s~22p transitions of the lithium isoelectronic sequence from Z= 11 to 20 are calculated by using the energies and the multiconfiguration interaction wavefunctions obtained from a full core plus correlation (FCPC) method. In most cases, the agreementbetween the oscillator strengths values from the length and velocity formula is up to four or fivedigit. Our results are aiso in good agreement with previous theoretical data available in theliterature. 展开更多
关键词 oscillator strengths lithium isoelectronic sequence full core pluscorrelation method
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Probability of Deuteron-Plasmon Fusion at Room Temperature within Micro-cracks of Crystalline Lattices with Deuterium Loading
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作者 Fulvio Frisone 《Journal of Energy and Power Engineering》 2017年第7期446-449,共4页
This communication seeks to demonstrate that, at room temperature, the deformation of the crystalline lattice can influence the process of interaction of deuterons introduced within it. Calculations of this probabilit... This communication seeks to demonstrate that, at room temperature, the deformation of the crystalline lattice can influence the process of interaction of deuterons introduced within it. Calculations of this probability, in fact, showed an increase of at least 2-3 orders of magnitude with respect to the probability of fusion on the surface of the lattice. These phenomena open the way to the theoretical hypothesis of a kind of chain reaction, as a result of the deuterium loading and catalysed by micro-cracks formed in the structure by micro-explosions, can favour the process. 展开更多
关键词 Deuteron-plasmon MICRO-CRACKS lattice defects.
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Effect of a Novel Nucleating Agent on Isothermal Crystallization of Poly(L-lactic acid) 被引量:7
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作者 WEN Liang XIN Zhong 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第6期899-904,共6页
The effect of a novel active nucleating agent(TBC8-eb) on the isothermal crystallization of poly(L-lactic acid) (PLLA) was studied by differential scanning calorimetry(DSC) and Fourier transform infrared spectroscopy(... The effect of a novel active nucleating agent(TBC8-eb) on the isothermal crystallization of poly(L-lactic acid) (PLLA) was studied by differential scanning calorimetry(DSC) and Fourier transform infrared spectroscopy(FTIR) . The analysis on kinetics demonstrates that TBC8-eb can not only accelerate the crystallization rate but also transform most of the original spherulite crystals of PLLA into sheaf-like crystals. Furthermore,the free energy of folding(σe) of PLLA and PLLA with TBC8-eb is 0.15 and 0.06 J·m-2,respectively,which suggests that the addition of TBC8-eb favors the regular folding of molecule chains in the crystallization of PLLA,improv-ing its crystallization rate. The FTIR results show that TBC8-eb can accelerate the conformational ordering of PLLA in the isothermal crystallization. The conformational ordering of PLLA nucleated with TBC8-eb begins with the interchain interaction of CH3,and then a short helix emerges where a couple of CH3 groups interact. 展开更多
关键词 CRYSTALLIZATION poly(L-lactic acid) nucleating agent
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Pellet Injection from High Field Side in ITER
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作者 DENG Baiquan PENG Lilin 《Southwestern Institute of Physics Annual Report》 2004年第1期88-92,共5页
Core fueling is plasmas to reach enhanced confinement regime and elevate output fusion power. However it is not easy to do so. Making use of the 2-D Kuteev lentil model, including kinetic effects, we find that existin... Core fueling is plasmas to reach enhanced confinement regime and elevate output fusion power. However it is not easy to do so. Making use of the 2-D Kuteev lentil model, including kinetic effects, we find that existing pellet injection techniques will not meet core-fueling requirements for ITER-FEAT. A pressureas high as 254 MPa should be applied to a pellet accelerator 200 cm-long single-stage pneumatic gun, in order to accelerate a pellet of radius rpo = 0. 5 cm to a velocity of νp0, 24 × 10^5cm·s^-1 penetrating 100 cm into the ITER plasma core. 展开更多
关键词 Pellet injection High field side ITER
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Genome-wide survey and analysis of microsatellites in the Pacific oyster genome: abundance, distribution, and potential for marker development
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作者 王家丰 亓海刚 +1 位作者 李莉 张国范 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第1期8-21,共14页
Microsatellites are a ubiquitous component of the eukaryote genome and constitute one of the most popular sources of molecular markers for genetic studies. However, no data are currently available regarding microsatel... Microsatellites are a ubiquitous component of the eukaryote genome and constitute one of the most popular sources of molecular markers for genetic studies. However, no data are currently available regarding microsatellites across the entire genome in oysters, despite their importance to the aquaculture industry. We present the fi rst genome-wide investigation of microsatellites in the Pacifi c oyster Crassostrea gigas by analysis of the complete genome, resequencing, and expression data. The Pacifi c oyster genome is rich in microsatellites. A total of 604 653 repeats were identifi ed, in average of one locus per 815 base pairs(bp). A total of 12 836 genes had coding repeats, and 7 332 were expressed normally, including genes with a wide range of molecular functions. Compared with 20 different species of animals, microsatellites in the oyster genome typically exhibited 1) an intermediate overall frequency; 2) relatively uniform contents of(A)n and(C)n repeats and abundant long(C)n repeats(≥24 bp); 3) large average length of(AG)n repeats; and 4) scarcity of trinucleotide repeats. The microsatellite-fl anking regions exhibited a high degree of polymorphism with a heterozygosity rate of around 2.0%, but there was no correlation between heterozygosity and microsatellite abundance. A total of 19 462 polymorphic microsatellites were discovered, and dinucleotide repeats were the most active, with over 26% of loci found to harbor allelic variations. In all, 7 451 loci with high potential for marker development were identifi ed. Better knowledge of the microsatellites in the oyster genome will provide information for the future design of a wide range of molecular markers and contribute to further advancements in the fi eld of oyster genetics, particularly for molecular-based selection and breeding. 展开更多
关键词 microsatellites GENOME Pacific oyster Crassostrea gigas
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