A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRN...A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios： Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows： KB culture medium （peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL）; temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% （v/v）.展开更多
This study was conducted to screen the commercial herbicides for algae control in the aquarium. Three herbicides of ametryn, atrazine and metribuzine were tested at concentrations ranging from 0.625 to 10.00 ppm. It i...This study was conducted to screen the commercial herbicides for algae control in the aquarium. Three herbicides of ametryn, atrazine and metribuzine were tested at concentrations ranging from 0.625 to 10.00 ppm. It is found that ametryn was the most effective herbicide that inhibited algal growth. Ametryn concentration that caused 50% inhibition on algae growth after exposure for 21 days was 0.335 ppm. Survival rate of oruamental fish, Harlequin rasbora （Trigonostigma heteromorpha） and growth rate of aquatic plant （Elodea canadensis） exposed to 10 ppm ametryn were compared with those exposed to algaecide. The result showed that there was no significant difference in survival rate of Harlequin rasbora when exposed to ametryn, algaecide and dechlorinized tap water （control）. However, growth rate of E. canadensis was lower after exposure of ametryn compared to those exposed to algaecide and tap water. These results suggest that ametryn has potential to be used as an algal inhibitor in aquarium.展开更多
In order to develop a model for screening the agonists of human β2-adrenoceptor from Chinese medicinal herbs extracts, we used a cell-based functional assay based on a common G protein-coupled receptor （GPCR） regul...In order to develop a model for screening the agonists of human β2-adrenoceptor from Chinese medicinal herbs extracts, we used a cell-based functional assay based on a common G protein-coupled receptor （GPCR） regulation mechanism and destabilized enhanced green fluorescent protein （d2EGFP） reporter gene technique. The positive cell clone was confirmed by real-time polymerase chain reaction （PCR） and imaging analysis. To assess the value of this model, we screened over 2000 high performance liquid chromatography （HPLC）-fractionated samples from the ethanol extracts of Chinese medicinal herbs. Six fractions （isolated from Panax japonicus, Veratrum nigrum, Phellodendron amurense, Fructus Aurantii lmmaturus, Chaenomeles speciosa, and Dictamnus dasycarpus） showed significant effects on active reporter gene expression, three of which （isolated from Phellodendron amurense, Fructus Aurantii lmmaturus, and Chaenomeles speciosa） were selected for further concentration response analysis and the half maximal effective concentration （EC1/2 max） values were 4.2, 2.7, and 4.8 μg/ml, respectively. Therefore, this reporter gene assay was suitable for screening β2-adrenoceptor agonists. The results suggest that the six herbal extracts are the possible agonists of β2-adrenoceptor.展开更多
基金supported by the Scientific Research Foundation of Xiamen Marine Research and Development Institute (No. K10102 (1))
文摘A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios： Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows： KB culture medium （peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL）; temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% （v/v）.
文摘This study was conducted to screen the commercial herbicides for algae control in the aquarium. Three herbicides of ametryn, atrazine and metribuzine were tested at concentrations ranging from 0.625 to 10.00 ppm. It is found that ametryn was the most effective herbicide that inhibited algal growth. Ametryn concentration that caused 50% inhibition on algae growth after exposure for 21 days was 0.335 ppm. Survival rate of oruamental fish, Harlequin rasbora （Trigonostigma heteromorpha） and growth rate of aquatic plant （Elodea canadensis） exposed to 10 ppm ametryn were compared with those exposed to algaecide. The result showed that there was no significant difference in survival rate of Harlequin rasbora when exposed to ametryn, algaecide and dechlorinized tap water （control）. However, growth rate of E. canadensis was lower after exposure of ametryn compared to those exposed to algaecide and tap water. These results suggest that ametryn has potential to be used as an algal inhibitor in aquarium.
基金Project (No. 30873103) supported by the National Natural Science Foundation of China
文摘In order to develop a model for screening the agonists of human β2-adrenoceptor from Chinese medicinal herbs extracts, we used a cell-based functional assay based on a common G protein-coupled receptor （GPCR） regulation mechanism and destabilized enhanced green fluorescent protein （d2EGFP） reporter gene technique. The positive cell clone was confirmed by real-time polymerase chain reaction （PCR） and imaging analysis. To assess the value of this model, we screened over 2000 high performance liquid chromatography （HPLC）-fractionated samples from the ethanol extracts of Chinese medicinal herbs. Six fractions （isolated from Panax japonicus, Veratrum nigrum, Phellodendron amurense, Fructus Aurantii lmmaturus, Chaenomeles speciosa, and Dictamnus dasycarpus） showed significant effects on active reporter gene expression, three of which （isolated from Phellodendron amurense, Fructus Aurantii lmmaturus, and Chaenomeles speciosa） were selected for further concentration response analysis and the half maximal effective concentration （EC1/2 max） values were 4.2, 2.7, and 4.8 μg/ml, respectively. Therefore, this reporter gene assay was suitable for screening β2-adrenoceptor agonists. The results suggest that the six herbal extracts are the possible agonists of β2-adrenoceptor.
