早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exp...早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选获得的核酸适配体,能够高特异性、高亲和力地与多种靶标(包括小分子、蛋白质、细胞、组织、微生物等)结合,为肝癌的特异识别、早期诊断、病理分型提供了有效方法。本研究主要阐述以肝癌细胞系、肝癌血清、临床肝癌组织为靶标的核酸适配体的筛选研究进展。展开更多
Aerobic glycolysis,also known as the Warburg effect,is a hallmark of cancer and essential for metabolism in malignancies,but its regulation and modulation in cancer cells remain poorly understood.Here,using large-scal...Aerobic glycolysis,also known as the Warburg effect,is a hallmark of cancer and essential for metabolism in malignancies,but its regulation and modulation in cancer cells remain poorly understood.Here,using large-scale functional screening,we identified a tumor-associated and broadly expressed oncogenic long noncoding RNA LINC00973.Notably,knocking down LINC00973 significantly inhibits the proliferation of multiple types of cancer cells and reduces tumor growth in vivo.Mechanistically,LINC00973 directly binds to lactate dehydrogenase A(LDHA),an essential glycolytic enzyme,and enhances its enzymatic activity,thereby promoting glycolysis.Clinically,high expression of LINC00973 is significantly associated with poor prognosis in many types of human cancers.This work demonstrates that LINC00973 modulates cancer-specific regulation of the Warburg effect,and may represent a potential target for broad-acting anti-cancer therapies.展开更多
文摘早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选获得的核酸适配体,能够高特异性、高亲和力地与多种靶标(包括小分子、蛋白质、细胞、组织、微生物等)结合,为肝癌的特异识别、早期诊断、病理分型提供了有效方法。本研究主要阐述以肝癌细胞系、肝癌血清、临床肝癌组织为靶标的核酸适配体的筛选研究进展。
基金This work was supported by the National Natural Science Foundation of China(31371314 and 81673460)Sichuan Youth Science and Technology Innovation Research Team of Experimental Formulology(2020JDTD0022).
文摘Aerobic glycolysis,also known as the Warburg effect,is a hallmark of cancer and essential for metabolism in malignancies,but its regulation and modulation in cancer cells remain poorly understood.Here,using large-scale functional screening,we identified a tumor-associated and broadly expressed oncogenic long noncoding RNA LINC00973.Notably,knocking down LINC00973 significantly inhibits the proliferation of multiple types of cancer cells and reduces tumor growth in vivo.Mechanistically,LINC00973 directly binds to lactate dehydrogenase A(LDHA),an essential glycolytic enzyme,and enhances its enzymatic activity,thereby promoting glycolysis.Clinically,high expression of LINC00973 is significantly associated with poor prognosis in many types of human cancers.This work demonstrates that LINC00973 modulates cancer-specific regulation of the Warburg effect,and may represent a potential target for broad-acting anti-cancer therapies.
