The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs...The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.展开更多
There have been a great many of studies on the pointed representations of fi- nite-dimensional sanple Lie algebras.cf.[1][2]etc.In this paper we give a new proof of an impottant Lemma,and from this we derive our main ...There have been a great many of studies on the pointed representations of fi- nite-dimensional sanple Lie algebras.cf.[1][2]etc.In this paper we give a new proof of an impottant Lemma,and from this we derive our main result:Irreducible pointed modules of finite -dimesional simple Lie algebras are all Harish-Chandra modules.展开更多
Abstract： Simple sequence repeat （SSR） analysis was used to study the genotype relation among ten different olives varieties from Al-Zafrania and A1-Mosel station ministry of agriculture/Iraq Shami, Sorani, Manzenl...Abstract： Simple sequence repeat （SSR） analysis was used to study the genotype relation among ten different olives varieties from Al-Zafrania and A1-Mosel station ministry of agriculture/Iraq Shami, Sorani, Manzenllo, Qaysi, Arbqween, Jlot （Labeeb）, Baashiqi, Dahkan, Nepali, Khodeiri, Fifteen SSR loci were studied and produced 239 amplified fragment. Two hundred and thirty seven of these loci （99.16%） were polymorphic over all the genotypes tested. Dendrogram and matrix of similarity were obtained by the Unweighted Pair-Group Method analysis （UPGMA）. Study showed two groups： group A： Nepali, Arbqween, and group B： divided in two sub groups （sub group B 1： Jlot, Dahkan, sub group B2： other cultivar）, while the genotype relation according to phenotype was confused. SSR has a better molecular marker than other molecular technique for detecting genetic relationship among cultivars, and help in known the pedigree of relatives and ancestors.展开更多
The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea E...The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.展开更多
We investigate the low-energy electronic structure ofa Weyl semimetal quantum dot （QD） with a simple model Hamiltonian with only two Weyl points. Distinguished from the semiconductor and topological insulator QDs, t...We investigate the low-energy electronic structure ofa Weyl semimetal quantum dot （QD） with a simple model Hamiltonian with only two Weyl points. Distinguished from the semiconductor and topological insulator QDs, there exist both surface and bulk states near the Fermi level in Weyl semimetal QDs. The surface state, distributed near the side surface of the QD, contributes a circular persistent current, an orbital magnetic moment, and a chiral spin polarization with spin-current locking. There are always surface states even for a strong magnetic field, even though a given surface state gradually evolves into a Landau level with increasing magnetic field. It indicates that these unique properties can be tuned via the QD size. In addition, we show the correspondence to the electronic structures of a three-dimensional Weyl semimetal, such as Wey[ point and Fermi arc. Because a QD has the largest surface-to-volume ratio, it provides a new platform to verify Weyl semimetal by separating and detecting the signals of surface states. Besides, the study of Weyl QDs is also necessary for potential applications in nanoelectronics.展开更多
基金supported by the grants from the Science and Technology Development Program of Shandong Province (2009GG10005013)Doctoral Program of Ministry of Education of China (20090132110018)
文摘The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.
文摘There have been a great many of studies on the pointed representations of fi- nite-dimensional sanple Lie algebras.cf.[1][2]etc.In this paper we give a new proof of an impottant Lemma,and from this we derive our main result:Irreducible pointed modules of finite -dimesional simple Lie algebras are all Harish-Chandra modules.
文摘Abstract： Simple sequence repeat （SSR） analysis was used to study the genotype relation among ten different olives varieties from Al-Zafrania and A1-Mosel station ministry of agriculture/Iraq Shami, Sorani, Manzenllo, Qaysi, Arbqween, Jlot （Labeeb）, Baashiqi, Dahkan, Nepali, Khodeiri, Fifteen SSR loci were studied and produced 239 amplified fragment. Two hundred and thirty seven of these loci （99.16%） were polymorphic over all the genotypes tested. Dendrogram and matrix of similarity were obtained by the Unweighted Pair-Group Method analysis （UPGMA）. Study showed two groups： group A： Nepali, Arbqween, and group B： divided in two sub groups （sub group B 1： Jlot, Dahkan, sub group B2： other cultivar）, while the genotype relation according to phenotype was confused. SSR has a better molecular marker than other molecular technique for detecting genetic relationship among cultivars, and help in known the pedigree of relatives and ancestors.
基金supported by the Zhejiang Provincial Science and Technol-ogy Program (No. 2007C32013)the Zhejiang Provincial Natural Science Foundation (No. Y3090660) of China
文摘The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.
基金supported by the National Natural Science Foundation of China(Grants Nos.11747122,11274364,and 11574007)the National Basic Research Program of China(Grant Nos.2017YFA0303301,and2015CB921102)+1 种基金the Natural Science Foundation of Shandong Province(Grant No.ZR2018PA007)the Doctoral Foundation of University of Jinan(Grant No.160100147)
文摘We investigate the low-energy electronic structure ofa Weyl semimetal quantum dot （QD） with a simple model Hamiltonian with only two Weyl points. Distinguished from the semiconductor and topological insulator QDs, there exist both surface and bulk states near the Fermi level in Weyl semimetal QDs. The surface state, distributed near the side surface of the QD, contributes a circular persistent current, an orbital magnetic moment, and a chiral spin polarization with spin-current locking. There are always surface states even for a strong magnetic field, even though a given surface state gradually evolves into a Landau level with increasing magnetic field. It indicates that these unique properties can be tuned via the QD size. In addition, we show the correspondence to the electronic structures of a three-dimensional Weyl semimetal, such as Wey[ point and Fermi arc. Because a QD has the largest surface-to-volume ratio, it provides a new platform to verify Weyl semimetal by separating and detecting the signals of surface states. Besides, the study of Weyl QDs is also necessary for potential applications in nanoelectronics.
