遗传密码的进化稳定性

本文评述了遗传密码的进化有关的物理问题。主要讨论了 :1 )从进化稳定性的观点研究遗传密码的简并规则 ,证明了现有密码表中多重态的简并规则是突变危险性极小的 ;2 )从进化稳定性的观点研究了遗传密码表中氨基酸和终止密码子的排布 ,导出了总体突变危险性极小的表 ,并证明了标准密码表是在某些约束 (这些约束反映了密码形成时期的初始条件 )下总体突变危险性极小的表 ;3)密码表中 2 0种氨基酸和终止密码简并度的分布 。

Application of Abnormal Touchdown PCR with High Degeneracy Primer in Amplification of Large-Family Genes

[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional touchdown PCR and the optimized abnormal touchdown PCR were respectively carried out to amplify the genomic DNA of Cyprinus carpio.[Result] Only one evident electrophoretic band and a few Sox genes were obtained by using normal PCR;no obvious electrophoretic band but dispersive product was obtained by normal touchdown PCR;ideal result was obtained by the abnormal touchdown PCR that three evident electrophoretic bands and much more Sox genes were amplified.[Conclusion] The research provided theoretical basis for the optimization and selection of PCR amplification conditions of the large-family genes.

遗传密码的逻辑

遗传密码是自然界的一项伟大创造。理论物理学教授罗辽复引进密码字典突变危险性的概念,成功地导出密码的简并规则、简并多重态的分布及排列规则,受到国际同行关注。