To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCE...To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCECs) on it (under the effect ofastrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelialelectrical resistance (TEER). Results The plate insert has a stability of at least 15 d underculture condition. TEER increased significantly under co-culture condition from (66.1 +- 13.3)Ωcm^2 to (182.2 +- 6.7) Ωcm^2. Conclusion This micropore membrane culture plate insert can beeasily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable andrecyclable. It follows that the plate insert is a useful tool for co-culture and the relatedresearch fields.展开更多
In tube hydroforming with axial feeding,under the effect of coupled internal pressure and axial stress,wrinkles often occur and affect the forming results.Wrinkling behavior of an AZ31B magnesium alloy tube was experi...In tube hydroforming with axial feeding,under the effect of coupled internal pressure and axial stress,wrinkles often occur and affect the forming results.Wrinkling behavior of an AZ31B magnesium alloy tube was experimentally investigated with different loading paths at different temperatures.Features of wrinkles,including shape,radius and width,were acquired from the experiments,as well as the thickness distribution.Numerical simulations were carried out to reveal the stress state during warm hydroforming,and then the strain history of material at the top and bottom of the wrinkles were analyzed according to the stress tracks and yielding ellipse.Finally,effects of loading paths on expansion ratio limit of warm hydroforming were analyzed.It is verified that at a certain temperature,expansion ratio limit can be increased obviously by applying a proper loading path and realizing enough axial feeding.展开更多
An important consideration when using hot-dip galvanized tubular structures is the uncertainty of the joint behaviour due to the possible reduction in the global joint resistance produced by the vent holes required fo...An important consideration when using hot-dip galvanized tubular structures is the uncertainty of the joint behaviour due to the possible reduction in the global joint resistance produced by the vent holes required for the galvanizing process. This paper assesses the effect on the joint strength of the angle between the brace members and the chord in a K- or N-joints made with rectangular hollow sections. The study is focused on the case when those brace members include characteristic holes required for the hot-dip galvanizing process. To accomplish the objective of the proposed work, some tests on full-scale K- and N-joints, including angles of 35°, 45°, 55° and 90°, were carried out. The experimental work was complemented by a validated numerical simulation in order to give some design recommendations and to extend the research to other joint configurations.展开更多
The venom apparatus in the large carpenter bees, Xylocopafenestrata （Fabricius） （Hymenoptera： Apidae） has been studied It consists of typical parts, i.e., the venom sac, free filament, Dufour gland, sting shaft a...The venom apparatus in the large carpenter bees, Xylocopafenestrata （Fabricius） （Hymenoptera： Apidae） has been studied It consists of typical parts, i.e., the venom sac, free filament, Dufour gland, sting shaft and accessory sclerites. It is highly sclerotized and concealed by the 7th abdominal tergite and sternite. The venom sac serves as a reservoir for the venom which synthesized by the convoluted gland present in it, which is remarkably uniform to that of ants. The median duct extends from lower portion of venom sac, which measures 2.5 ±0.17 mm （mean ＋ SD） （n = 20） in length. The Dufour gland is a tube-like structure which is inserted in the sting-bulb. The triangular plate is attached at terminal portion of rami, which is in turn attached with lancets. The gonostyli are paired long and tubular structure with 5.29 ± 0.23 mm （n = 20） in length. The sting length is 5.29 ±0.23 mm （n = 20）. The fulcral arm is well-developed with an inverse Y-shaped structure present beneath the sting-bulb. Barbs on the sting shaft are weakly developed in X fenestrata but well developed in ants. The venom apparatus ofX. fenestrata when compared is similar to the bees but differs from that of the wasps and ants.展开更多
基金NationalMedicine 863Project (No .2 0 0 2AA2Z3 43C)
文摘To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCECs) on it (under the effect ofastrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelialelectrical resistance (TEER). Results The plate insert has a stability of at least 15 d underculture condition. TEER increased significantly under co-culture condition from (66.1 +- 13.3)Ωcm^2 to (182.2 +- 6.7) Ωcm^2. Conclusion This micropore membrane culture plate insert can beeasily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable andrecyclable. It follows that the plate insert is a useful tool for co-culture and the relatedresearch fields.
基金Project(NCET-07-0237)supported by the Program for New Century Excellent Talents in University,China
文摘In tube hydroforming with axial feeding,under the effect of coupled internal pressure and axial stress,wrinkles often occur and affect the forming results.Wrinkling behavior of an AZ31B magnesium alloy tube was experimentally investigated with different loading paths at different temperatures.Features of wrinkles,including shape,radius and width,were acquired from the experiments,as well as the thickness distribution.Numerical simulations were carried out to reveal the stress state during warm hydroforming,and then the strain history of material at the top and bottom of the wrinkles were analyzed according to the stress tracks and yielding ellipse.Finally,effects of loading paths on expansion ratio limit of warm hydroforming were analyzed.It is verified that at a certain temperature,expansion ratio limit can be increased obviously by applying a proper loading path and realizing enough axial feeding.
文摘An important consideration when using hot-dip galvanized tubular structures is the uncertainty of the joint behaviour due to the possible reduction in the global joint resistance produced by the vent holes required for the galvanizing process. This paper assesses the effect on the joint strength of the angle between the brace members and the chord in a K- or N-joints made with rectangular hollow sections. The study is focused on the case when those brace members include characteristic holes required for the hot-dip galvanizing process. To accomplish the objective of the proposed work, some tests on full-scale K- and N-joints, including angles of 35°, 45°, 55° and 90°, were carried out. The experimental work was complemented by a validated numerical simulation in order to give some design recommendations and to extend the research to other joint configurations.
文摘The venom apparatus in the large carpenter bees, Xylocopafenestrata （Fabricius） （Hymenoptera： Apidae） has been studied It consists of typical parts, i.e., the venom sac, free filament, Dufour gland, sting shaft and accessory sclerites. It is highly sclerotized and concealed by the 7th abdominal tergite and sternite. The venom sac serves as a reservoir for the venom which synthesized by the convoluted gland present in it, which is remarkably uniform to that of ants. The median duct extends from lower portion of venom sac, which measures 2.5 ±0.17 mm （mean ＋ SD） （n = 20） in length. The Dufour gland is a tube-like structure which is inserted in the sting-bulb. The triangular plate is attached at terminal portion of rami, which is in turn attached with lancets. The gonostyli are paired long and tubular structure with 5.29 ± 0.23 mm （n = 20） in length. The sting length is 5.29 ±0.23 mm （n = 20）. The fulcral arm is well-developed with an inverse Y-shaped structure present beneath the sting-bulb. Barbs on the sting shaft are weakly developed in X fenestrata but well developed in ants. The venom apparatus ofX. fenestrata when compared is similar to the bees but differs from that of the wasps and ants.
