The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, tempe...The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were: 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.展开更多
The magnetic poly-（methacrylate-divinyl benzene） microspheres with micron size were synthesized by modified suspension polymerization method. Adsorption of Cr（VI） from aqueous solution by magnetic poly-（MA-DVB） ...The magnetic poly-（methacrylate-divinyl benzene） microspheres with micron size were synthesized by modified suspension polymerization method. Adsorption of Cr（VI） from aqueous solution by magnetic poly-（MA-DVB） microspheres with surface amination was investigated. The adsorption processes were carried out under diversified conditions of pH value, adsorption time and temperature to evaluate the perlbrmance of the magnetic microspheres. The optimum pH value for Cr（VI） adsorption was found as 3. The adsorption capacity increased with adsorption time and attained an optimum at 60 rain. The adsorption processes for magnetic microspheres was endothermic reaction, and the adsorption capacity increased with increasing temperature.展开更多
The distribution effect of electric field on the alignment and attachment of carbon nanotubes （CNTs） were investigated. The experimental results were compared with the simulation results according to three different...The distribution effect of electric field on the alignment and attachment of carbon nanotubes （CNTs） were investigated. The experimental results were compared with the simulation results according to three different shaped electrodes. In previous simulation, the round shaped electrodes were expected to be more effective for aligning and attaching a single CNT between two electrodes than conical or rectangular shaped electrodes. To verify the simulation results, three different shaped electrodes were introduced and a single multi-walled carbon nanotube （MWNT） was attached. The optimal conditions for aligning and attaching MWNTs such as the frequency, applied voltage and concentration of MWNTs solution were investigated. Through repeated experiments, frequency of 100 kHz-10 MHz, applied voltage of 0.3-1.3 V^s/~m, concentration of 5 ktg/mL in MWNTs solution were obtained as a possible condition range to attach MWNTs. Under these conditions, the yield of MWNTs attachment between two electrodes was up to 70%. In previous simulation, furthermore, it was verified that the size of the stable or quasi-stable region made CNTs aligned and attached on different shaped electrodes from the comparison of the experimental and simulation results. Most single MWNT attachment was accomplished on the round shaped electrodes.展开更多
A new fluorescence silver nanosensor assisted by surfactant has been synthesized and applied to ultra trace nickel determination. Operational variables which influence nanomaterial synthesis have been studied and opti...A new fluorescence silver nanosensor assisted by surfactant has been synthesized and applied to ultra trace nickel determination. Operational variables which influence nanomaterial synthesis have been studied and optimized. Synthesis was very fast and simple using non polluting solvents; silver chemical reduction was carried out at room temperature. Spectroscopic studies were carried out in order to assure the uniformed of nanomaterial obtained. Fluorescent signal of silver nanoparticles resulted enhanced in presence of Ni（II）. At optimal experimental conditions, a detection limit of 0.036 pg＇L1 and quantification limit 0.12 pg＇L~ were obtained. The calibration sensitivity was 2 x 1014 L.pg-l.cm1 for the new methodology, with a range of linearity of six orders of magnitude between 0.12 and 2.93 × 10^5 pg L^-1. The tolerance levels for potential interferent ions were studied with good results. The proposed methodology represents a promising approach for Ni（II） traces quantification due to its low operation cost, simplicity of instrumentation, high sampling speed and non-polluting solvents.展开更多
The understanding of enzymatic saccharification of pretreated lignocellulosic material is of great importance. There are several important commercially available enzymes in the market that are used for this purpose. T...The understanding of enzymatic saccharification of pretreated lignocellulosic material is of great importance. There are several important commercially available enzymes in the market that are used for this purpose. The conditions of pH and temperature performance of any particular enzyme are very well defined and it is clearly indicated by its manufacturer and it depends on the type of enzyme or enzymes in the complex pool. It is well know that commercial cellulases work best at pH around 4.8-5.0 and as a consequence this is widely used in the industry and the literature. In this study it was found that optimum pH of cellulases is different than that recommended by its manufacturer at higher solids load saccharification. The optimum pH changes depending on the consistency or solids loads of the matrix where the enzyme is acting upon. Steam exploded corn stover was tested with cellulases and xylanases at different pH, consistencies and ionic strength. Results showed that the optimum pH at lower consistency （1% w/w） is the same as the one recommended by the manufacturer and in the literature; however at higher consistency the value obtained was higher （pH 5.5 to pH 6.5） instead ofpH 4.8. The difference could represent up to 30-50% higher yields and hence of great importance for the economics of second generation fuel production. An explanation of this behavior could be associated with the Donnan effect theory. This effect indicates that the presence of charged groups in the fiber matrix creates a pH gradient within the slurry. If the charged groups are negatively charged this would create a local or internal pH lower than the surrounding liquid pH. This could explain why by reducing the concentration of H^＋ higher enzymatic conversion yields were observed.展开更多
Attempts had been made to synthesize Al2O3-2SiO2 nanopowders by sol-gel method with tetraethoxysilane（TEOS） and aluminum nitrate（ANN） as the starting materials.DTS,TEM,SEM and BET were employed to study the effect...Attempts had been made to synthesize Al2O3-2SiO2 nanopowders by sol-gel method with tetraethoxysilane（TEOS） and aluminum nitrate（ANN） as the starting materials.DTS,TEM,SEM and BET were employed to study the effects of process parameters on the size,specific surface area and structure（morphology） of powders.The alkali-activation reactivity of the powders was tested for manufacturing geopolymers and their hydrothermal reactions were performed for fabricating zeolites.The results show that the optimum process parameters and drying method for preparing Al2O3-2SiO2 nanopowders are as follows：the molar ratio of water and ethanol to TEOS are 0：1 and 12：1 respectively at synthetic temperature of 50 ℃ and the drying method is azeotropic distillation with microwave drying.The average particle diameters of the powders were about 70 nm and the largest BET specific surface area was up to 669 m^2·g^-1.The compressive strength of the geopolymer and the calcium exchange capacity（by CaCO3） of NaA zeolite prepared with the powders reached to 29 MPa and 366 m^2·g^-1 respectively.展开更多
基金Supported by the National High Technology Research and Development Program of China (2006AA020101, 2007AA10Z360,2009AA03Z232)Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (2008BA163B07)
文摘The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were: 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.
基金Supported by the National Basic Research Program of China (2007CB613507)
文摘The magnetic poly-（methacrylate-divinyl benzene） microspheres with micron size were synthesized by modified suspension polymerization method. Adsorption of Cr（VI） from aqueous solution by magnetic poly-（MA-DVB） microspheres with surface amination was investigated. The adsorption processes were carried out under diversified conditions of pH value, adsorption time and temperature to evaluate the perlbrmance of the magnetic microspheres. The optimum pH value for Cr（VI） adsorption was found as 3. The adsorption capacity increased with adsorption time and attained an optimum at 60 rain. The adsorption processes for magnetic microspheres was endothermic reaction, and the adsorption capacity increased with increasing temperature.
基金Project supported by Pusan National University Research Grant, KoreaProject(2010-0008-276) supported by NCRC (National Core Research Center) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology
文摘The distribution effect of electric field on the alignment and attachment of carbon nanotubes （CNTs） were investigated. The experimental results were compared with the simulation results according to three different shaped electrodes. In previous simulation, the round shaped electrodes were expected to be more effective for aligning and attaching a single CNT between two electrodes than conical or rectangular shaped electrodes. To verify the simulation results, three different shaped electrodes were introduced and a single multi-walled carbon nanotube （MWNT） was attached. The optimal conditions for aligning and attaching MWNTs such as the frequency, applied voltage and concentration of MWNTs solution were investigated. Through repeated experiments, frequency of 100 kHz-10 MHz, applied voltage of 0.3-1.3 V^s/~m, concentration of 5 ktg/mL in MWNTs solution were obtained as a possible condition range to attach MWNTs. Under these conditions, the yield of MWNTs attachment between two electrodes was up to 70%. In previous simulation, furthermore, it was verified that the size of the stable or quasi-stable region made CNTs aligned and attached on different shaped electrodes from the comparison of the experimental and simulation results. Most single MWNT attachment was accomplished on the round shaped electrodes.
文摘A new fluorescence silver nanosensor assisted by surfactant has been synthesized and applied to ultra trace nickel determination. Operational variables which influence nanomaterial synthesis have been studied and optimized. Synthesis was very fast and simple using non polluting solvents; silver chemical reduction was carried out at room temperature. Spectroscopic studies were carried out in order to assure the uniformed of nanomaterial obtained. Fluorescent signal of silver nanoparticles resulted enhanced in presence of Ni（II）. At optimal experimental conditions, a detection limit of 0.036 pg＇L1 and quantification limit 0.12 pg＇L~ were obtained. The calibration sensitivity was 2 x 1014 L.pg-l.cm1 for the new methodology, with a range of linearity of six orders of magnitude between 0.12 and 2.93 × 10^5 pg L^-1. The tolerance levels for potential interferent ions were studied with good results. The proposed methodology represents a promising approach for Ni（II） traces quantification due to its low operation cost, simplicity of instrumentation, high sampling speed and non-polluting solvents.
文摘The understanding of enzymatic saccharification of pretreated lignocellulosic material is of great importance. There are several important commercially available enzymes in the market that are used for this purpose. The conditions of pH and temperature performance of any particular enzyme are very well defined and it is clearly indicated by its manufacturer and it depends on the type of enzyme or enzymes in the complex pool. It is well know that commercial cellulases work best at pH around 4.8-5.0 and as a consequence this is widely used in the industry and the literature. In this study it was found that optimum pH of cellulases is different than that recommended by its manufacturer at higher solids load saccharification. The optimum pH changes depending on the consistency or solids loads of the matrix where the enzyme is acting upon. Steam exploded corn stover was tested with cellulases and xylanases at different pH, consistencies and ionic strength. Results showed that the optimum pH at lower consistency （1% w/w） is the same as the one recommended by the manufacturer and in the literature; however at higher consistency the value obtained was higher （pH 5.5 to pH 6.5） instead ofpH 4.8. The difference could represent up to 30-50% higher yields and hence of great importance for the economics of second generation fuel production. An explanation of this behavior could be associated with the Donnan effect theory. This effect indicates that the presence of charged groups in the fiber matrix creates a pH gradient within the slurry. If the charged groups are negatively charged this would create a local or internal pH lower than the surrounding liquid pH. This could explain why by reducing the concentration of H^＋ higher enzymatic conversion yields were observed.
基金Supported by the National Natural Science Foundation of China (50962002,50602006)Opening Funds of State Key Laboratory of Chemical Resource Engineering of Beijing University of Chemical Technology (201008)
文摘Attempts had been made to synthesize Al2O3-2SiO2 nanopowders by sol-gel method with tetraethoxysilane（TEOS） and aluminum nitrate（ANN） as the starting materials.DTS,TEM,SEM and BET were employed to study the effects of process parameters on the size,specific surface area and structure（morphology） of powders.The alkali-activation reactivity of the powders was tested for manufacturing geopolymers and their hydrothermal reactions were performed for fabricating zeolites.The results show that the optimum process parameters and drying method for preparing Al2O3-2SiO2 nanopowders are as follows：the molar ratio of water and ethanol to TEOS are 0：1 and 12：1 respectively at synthetic temperature of 50 ℃ and the drying method is azeotropic distillation with microwave drying.The average particle diameters of the powders were about 70 nm and the largest BET specific surface area was up to 669 m^2·g^-1.The compressive strength of the geopolymer and the calcium exchange capacity（by CaCO3） of NaA zeolite prepared with the powders reached to 29 MPa and 366 m^2·g^-1 respectively.
