The breeding biology of the Spot-billed Pelican(Pelecanus philippensis)was investigated from September 2007 to September 2010 in Karaivetti Lake,Tamil Nadu,India.The Spot-billed Pelican breeds between November and Apr...The breeding biology of the Spot-billed Pelican(Pelecanus philippensis)was investigated from September 2007 to September 2010 in Karaivetti Lake,Tamil Nadu,India.The Spot-billed Pelican breeds between November and April at the Karaivetti Lake.It also breeds roughly during the same period at other breeding sites in Tamil Nadu,except for the Vedanthangal and Karikili bird sanctuaries,where pelicans arrives much earlier and their breeding activities start and end to some extent ahead of the other sites,i.e.,from September to March.In essence,the breeding season starts after the onset of the northeast monsoon in Tamil Nadu.A few days after their arrival,pelicans perform courtship display,form pairs,start nest construction and lay eggs.Courtship display is not as attractive and impressive as in other waterbirds.The clutch size varies from two to three eggs and the incubation period from 25 to 36 days.The fledging period varies from a minimum of 90 to a maximum of 102 days.The nest success rate of the Spot-billed Pelican at Karaivetti was 90.28% during the study period.展开更多
[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize a...[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.展开更多
基金India for funding the studythe Tamil Nadu Forest Department for granting me the required permission
文摘The breeding biology of the Spot-billed Pelican(Pelecanus philippensis)was investigated from September 2007 to September 2010 in Karaivetti Lake,Tamil Nadu,India.The Spot-billed Pelican breeds between November and April at the Karaivetti Lake.It also breeds roughly during the same period at other breeding sites in Tamil Nadu,except for the Vedanthangal and Karikili bird sanctuaries,where pelicans arrives much earlier and their breeding activities start and end to some extent ahead of the other sites,i.e.,from September to March.In essence,the breeding season starts after the onset of the northeast monsoon in Tamil Nadu.A few days after their arrival,pelicans perform courtship display,form pairs,start nest construction and lay eggs.Courtship display is not as attractive and impressive as in other waterbirds.The clutch size varies from two to three eggs and the incubation period from 25 to 36 days.The fledging period varies from a minimum of 90 to a maximum of 102 days.The nest success rate of the Spot-billed Pelican at Karaivetti was 90.28% during the study period.
基金Supported by Major Projects for Cultivation of New Varieties ofGenetically Modified Organisms (2009ZX08010-011B)
文摘[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.
