Two variants of diet composition were prepared to evaluate the susceptibility of ECBs to CrylAb toxin as follows： 1） 38-0600 Stonefly Heliothis Diet mixed with purified CrylAb protein and 2） 38-0600 Stonefly Heliot...Two variants of diet composition were prepared to evaluate the susceptibility of ECBs to CrylAb toxin as follows： 1） 38-0600 Stonefly Heliothis Diet mixed with purified CrylAb protein and 2） 38-0600 Stonefly Heliothis Diet mixed with lyophylized leaves of Bt maize MON 810-YieldGard. A method of sample preparation and extraction of Bt toxin for reproducible ELISA quantification were optimized. The qualitative DAS-ELISA kit from Agdia was optimized for use in quantitative analysis of Cry lAb toxin. The mortality of ECB larvae from the laboratory strain on the diet with CrylAb toxin did not differ significantly from the mortality on the diet with Bt maize leaves with the same rate of Cry lAb toxin. Similarly, the mortality of the ECB larvae from the field population on the diet with Bt maize leaves did not differ significantly from the mortality of ECB larvae from the laboratory strain on the same type of diet. Therefore, the incorporation of Bt maize leaves into the diet did not influence the efficacy of CrylAb toxin against ECBs. Using this method, a susceptibility of one field population of ECBs from the Czech Republic to CrylAb toxin was determined （LC50 of 2.16 μg of Cry 1Ab g^-1 of diet）.展开更多
A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Glirici...A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Gliricidia （Gliricidia sepium） leaf or rice bran on feed intake, feed digestibility and liveweight gain of Kacang goat given Mulato （Brachiaria mulato） grass. Twenty four male Kacang goat, approximately 13.4±0.6 kg in initial weight and 10 months of age, were housed in individual metabolic pen and allocated to one of three dietary treatments. The experimental design was a completely randomized block design, with three treatments and eight replicates （goats）. The treatments were Mulato grass ad libitum （M）, Mulato ＋ Gliricidia （1% weight/d on dry matter basis） （MG）, and Mulato ＋ rice bran （1% weight/d on dry matter basis） （MR）. The experiment was run for eight weeks period （two weeks for adaptation and and six weeks for measurement period, respectively）. Parameters measured were liveweight gain （LWG）, dry matter intake （DMI） and dry matter digestibility （DMD）. The result showed that dietary treatment significantly （P 〈 0.05） affected the LWG, DMI and DMD of Kacang goat. Goat given Mulato grass had the lowest LWG, DMI and DMD with value 58 g/d, 2.77% weight/d and 57.61%, respectively. Goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran had a significant higher LWG （73 g/d and 76 g/d, respectively）, DMI （3.25% weight/d and 3.65% weight/d, respectively） and DMD （62.28% and 63.19%, respectively）. However, no significant difference （P 〉 0.05） between goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran. In conclusion, this study demonstrated that protein or energy contents of the diet influenced growth, feed intake and digestibility of Kacang goat.展开更多
文摘Two variants of diet composition were prepared to evaluate the susceptibility of ECBs to CrylAb toxin as follows： 1） 38-0600 Stonefly Heliothis Diet mixed with purified CrylAb protein and 2） 38-0600 Stonefly Heliothis Diet mixed with lyophylized leaves of Bt maize MON 810-YieldGard. A method of sample preparation and extraction of Bt toxin for reproducible ELISA quantification were optimized. The qualitative DAS-ELISA kit from Agdia was optimized for use in quantitative analysis of Cry lAb toxin. The mortality of ECB larvae from the laboratory strain on the diet with CrylAb toxin did not differ significantly from the mortality on the diet with Bt maize leaves with the same rate of Cry lAb toxin. Similarly, the mortality of the ECB larvae from the field population on the diet with Bt maize leaves did not differ significantly from the mortality of ECB larvae from the laboratory strain on the same type of diet. Therefore, the incorporation of Bt maize leaves into the diet did not influence the efficacy of CrylAb toxin against ECBs. Using this method, a susceptibility of one field population of ECBs from the Czech Republic to CrylAb toxin was determined （LC50 of 2.16 μg of Cry 1Ab g^-1 of diet）.
文摘A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Gliricidia （Gliricidia sepium） leaf or rice bran on feed intake, feed digestibility and liveweight gain of Kacang goat given Mulato （Brachiaria mulato） grass. Twenty four male Kacang goat, approximately 13.4±0.6 kg in initial weight and 10 months of age, were housed in individual metabolic pen and allocated to one of three dietary treatments. The experimental design was a completely randomized block design, with three treatments and eight replicates （goats）. The treatments were Mulato grass ad libitum （M）, Mulato ＋ Gliricidia （1% weight/d on dry matter basis） （MG）, and Mulato ＋ rice bran （1% weight/d on dry matter basis） （MR）. The experiment was run for eight weeks period （two weeks for adaptation and and six weeks for measurement period, respectively）. Parameters measured were liveweight gain （LWG）, dry matter intake （DMI） and dry matter digestibility （DMD）. The result showed that dietary treatment significantly （P 〈 0.05） affected the LWG, DMI and DMD of Kacang goat. Goat given Mulato grass had the lowest LWG, DMI and DMD with value 58 g/d, 2.77% weight/d and 57.61%, respectively. Goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran had a significant higher LWG （73 g/d and 76 g/d, respectively）, DMI （3.25% weight/d and 3.65% weight/d, respectively） and DMD （62.28% and 63.19%, respectively）. However, no significant difference （P 〉 0.05） between goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran. In conclusion, this study demonstrated that protein or energy contents of the diet influenced growth, feed intake and digestibility of Kacang goat.
