果蝇样品经固相萃取后,采用高效液相色谱-串联质谱法进行样品分析,检测条件用Endeavorsil^(TM)C_(18)色谱柱(100 mm×2.1 mm,1.8μm),以0.1%甲酸水-乙腈为流动相,流速为0.3 m L/min,柱温40℃;采用电喷雾离子源负离子多重反应监测模...果蝇样品经固相萃取后,采用高效液相色谱-串联质谱法进行样品分析,检测条件用Endeavorsil^(TM)C_(18)色谱柱(100 mm×2.1 mm,1.8μm),以0.1%甲酸水-乙腈为流动相,流速为0.3 m L/min,柱温40℃;采用电喷雾离子源负离子多重反应监测模式,内标标准工作曲线定量,建立了果蝇组织中15种多不饱和脂肪酸代谢产物——类二十烷酸的测定方法。结果显示,在2.5~200 ng/m L范围内呈良好的线性关系(r=0.991),回收率范围在89.3%~108.9%,日内精密度相对标准偏差(relative standard deviation,RSD)在1.2%~12.4%,日间精密度RSD在1.0%~15.0%,检出限0.1~2.6 ng/g,定量限0.3~8.7 ng/g。所建方法简便、准确、选择性高,为研究模式生物果蝇对多不饱和脂肪酸的代谢特性提供了科学依据。展开更多
Surfactant enhanced remediation is thought to be an effective method for the remediation of soils polluted with hydrophoblc organic compounds. Desorption of polycyclic aromatic hydrocarbons （PAHs） from an abandoned ...Surfactant enhanced remediation is thought to be an effective method for the remediation of soils polluted with hydrophoblc organic compounds. Desorption of polycyclic aromatic hydrocarbons （PAHs） from an abandoned manufactured gas plant （MGP） soil was evaluated using four eluting agents including Triton X-100 （TX100）, sodium dodecylbenzene sulfonate （SDBS）, rhamnolipid water solution （RWS） and rhamnolipid fermentation broth （RFB）. The weight solubilization ratios for acenaphthene and fluorene were in the order of TX100 〉 SDBS 〉 RWS 〉 RFB. The Sm value, which indicates the maximum amounts of surfactants adsorbed in the soil, was in the order of RWS 〉 RFB 〉 SDBS 〉 TX100. By using 8 g L-1 of TX100, SDBS and RWS and 100% of RFB, the T-PAHs removal for the MGP soil contaminated with 207.86 mg T-PAHs kg-1 dry soil was 48.0%, 45.7%, 1.9%, and 8.6%, respectively, while that decreased to 41.6%, 37%, 0.38%, and 1.3% for the soil contaminated with 3494.78 mg T-PAHs kg-1 dry soil. Only 8 g L-1 TX100 could remove all types of the 16 PAHs partly in the MGP soil, and the removal efficiencies of different PAHs ranged from 13% to 77.8%. The results of this study herein provide valuable information for the selection of TX100 surfactant for remediating PAH-contaminated soils in MGP.展开更多
基金supported by the National Natural Science Foundation of China(No.91939302,82070305)the Natural Science Foundation of Tianjin,China(No.19JCQNJC10100)。
基金supported by the National Natural Science Foundation of China(No.81730014,81800251)Tianjin Municipal Science and Technology Project,China(No.20JCQNJC00540)。
基金Supported by the National High Technology Research and Development Program(863 Program)of China(No.2012AA06A201)the Cooperation Program of the Beijing Branch of Chinese Academy of Sciences and the Beijing Academy of Science and Technology of China(No.PXM2010-178203-096006)
文摘Surfactant enhanced remediation is thought to be an effective method for the remediation of soils polluted with hydrophoblc organic compounds. Desorption of polycyclic aromatic hydrocarbons （PAHs） from an abandoned manufactured gas plant （MGP） soil was evaluated using four eluting agents including Triton X-100 （TX100）, sodium dodecylbenzene sulfonate （SDBS）, rhamnolipid water solution （RWS） and rhamnolipid fermentation broth （RFB）. The weight solubilization ratios for acenaphthene and fluorene were in the order of TX100 〉 SDBS 〉 RWS 〉 RFB. The Sm value, which indicates the maximum amounts of surfactants adsorbed in the soil, was in the order of RWS 〉 RFB 〉 SDBS 〉 TX100. By using 8 g L-1 of TX100, SDBS and RWS and 100% of RFB, the T-PAHs removal for the MGP soil contaminated with 207.86 mg T-PAHs kg-1 dry soil was 48.0%, 45.7%, 1.9%, and 8.6%, respectively, while that decreased to 41.6%, 37%, 0.38%, and 1.3% for the soil contaminated with 3494.78 mg T-PAHs kg-1 dry soil. Only 8 g L-1 TX100 could remove all types of the 16 PAHs partly in the MGP soil, and the removal efficiencies of different PAHs ranged from 13% to 77.8%. The results of this study herein provide valuable information for the selection of TX100 surfactant for remediating PAH-contaminated soils in MGP.
