Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enz...Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub strates.The biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.展开更多
The insecticidal protein from Pseudomonas pseudoaligenes was and exotoxin which had toxicity on locusts.In order to elucidate its molecular properties an amino acid sequence,the insecticidal protein was purified from ...The insecticidal protein from Pseudomonas pseudoaligenes was and exotoxin which had toxicity on locusts.In order to elucidate its molecular properties an amino acid sequence,the insecticidal protein was purified from the culture supernatant by ultrafiltration,ion\|exchange chromatography and gel filtration,and showed a single band on SDS\|PAGE.Analysis of the purified insecticidal protein dentified N\|terminal sequence of ten amino acid residues.Its polyclonal antibody was also obtained by immunizing rabbit with the insecticidal protein recovered form SDS\|PAGE gel.The antibody titer determined by ELISA method was 1∶12800,indicating that it had high reactivity.Western blot analysis revealed that the antibody was spectific to 26kD insecticidal protein,and did not cross\|react with other proteins produced by the bacterium,suggesting that a specific antibody with high titer was obtained and could be used for further investigations of the gene cloning and expression of insecticidal protein.展开更多
文摘Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub strates.The biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.
文摘The insecticidal protein from Pseudomonas pseudoaligenes was and exotoxin which had toxicity on locusts.In order to elucidate its molecular properties an amino acid sequence,the insecticidal protein was purified from the culture supernatant by ultrafiltration,ion\|exchange chromatography and gel filtration,and showed a single band on SDS\|PAGE.Analysis of the purified insecticidal protein dentified N\|terminal sequence of ten amino acid residues.Its polyclonal antibody was also obtained by immunizing rabbit with the insecticidal protein recovered form SDS\|PAGE gel.The antibody titer determined by ELISA method was 1∶12800,indicating that it had high reactivity.Western blot analysis revealed that the antibody was spectific to 26kD insecticidal protein,and did not cross\|react with other proteins produced by the bacterium,suggesting that a specific antibody with high titer was obtained and could be used for further investigations of the gene cloning and expression of insecticidal protein.
